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131.
Climate change is an urgent environmental issue with wide-ranging impacts on ecosystems and society. Microbes are instrumental in maintaining the balance between carbon (C) accumulation and loss in the biosphere, actively regulating greenhouse gas fluxes from vast reservoirs of organic C stored in soils, sediments and oceans. Heterotrophic microbes exhibit varying capacities to access, degrade and metabolise organic C—leading to variations in remineralisation and turnover rates. The present challenge lies in effectively translating this accumulated knowledge into strategies that effectively steer the fate of organic C towards prolonged sequestration. In this article, we discuss three ecological scenarios that offer potential avenues for shaping C turnover rates in the environment. Specifically, we explore the promotion of slow-cycling microbial byproducts, the facilitation of higher carbon use efficiency, and the influence of biotic interactions. The ability to harness and control these processes relies on the integration of ecological principles and management practices, combined with advances in economically viable technologies to effectively manage microbial systems in the environment. 相似文献
132.
Marina Di Domenico Daniela Pozzi Sara Palchetti Luca Digiacomo Rosamaria Iorio Carlo Astarita Alfonso Fiorelli Matteo Pierdiluca Mario Santini Marcella Barbarino Antonio Giordano Angelina Di Carlo Luigi Frati Morteza Mahmoudi Giulio Caracciolo 《Journal of cellular physiology》2019,234(6):9378-9386
Lung cancer (LC) is the most common type of cancer and the second cause of death worldwide in men and women after cardiovascular diseases. Non-small-cell lung cancer (NSCLC) is the most frequent type of LC occurring in 85% of cases. Developing new methods for early detection of NSCLC could substantially increase the chances of survival and, therefore, is an urgent task for current research. Nowadays, explosion in nanotechnology offers unprecedented opportunities for therapeutics and diagnosis applications. In this context, exploiting the bio-nano-interactions between nanoparticles (NPs) and biological fluids is an emerging field of research. Upon contact with biofluids, NPs are covered by a biomolecular coating referred to as “biomolecular corona” (BC). In this study, we exploited BC for discriminating between NSCLC patients and healthy volunteers. Blood samples from 10 NSCLC patients and 5 subjects without malignancy were allowed to interact with negatively charged lipid NPs, leading to the formation of a BC at the NP surface. After isolation, BCs were characterized by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE). We found that the BCs of NSCLC patients was significantly different from that of healthy individuals. Statistical analysis of SDS-PAGE results allowed discriminating between NSCLC cancer patients and healthy subjects with 80% specificity, 80% sensitivity and a total discriminate correctness rate of 80%. While the results of the present investigation cannot be conclusive due to the small size of the data set, we have shown that exploitation of the BC is a promising approach for the early diagnosis of NSCLC. 相似文献
133.
Atilla Arslan Emine Arslan Ahmad Mahmoudi Anna Bannikova 《Zoology in the Middle East.》2017,63(1):1-7
The most genetically divergent populations of the European snow vole Chionomys nivalis occupy the extreme eastern range of the Black Sea and the Caspian Sea. It was recently suggested that subspecies C. n. spitzenbergerae from the Central Taurus Mountains (Turkey) represents a highly divergent lineage of C. nivalis from the Alada? Range which induced us to address its status by examining topotype specimens. Two females karyotyped displayed 54 mitotic chromosomes of 25 acrocentric autosomal pairs. One of the smallest autosomal pairs was heteromorphic in both specimens, consisting of a submetacentric and an acrocentric chromosomes (NFa = 53). Cytochrome b sequences however unambiguously clustered both individuals with reference sequences from C. nivalis from Turkey. We conclude that a deviant haplotype from the Alada? Range does not represent C. n. spitzenbergerae. Its taxonomic identity was not resolved in our study. Further attention should be devoted to snow voles from Central Anatolia and Western Iran, which are characterized by cranial peculiarities. 相似文献
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136.
Mohsen Nikseresht Maryam Shahverdi Mehdi Dehghani Hassan Abidi Reza Mahmoudi Ghasem Ghalamfarsa Leila Manzouri Saeid Ghavami 《Journal of cellular biochemistry》2019,120(2):1924-1931
Introduction : Autophagy is a mechanism that is involved in the regulation of cellular life, apoptosis, and stemness while its intervening genes play important functions in various cancers including lung cancer. ATG5 is one of the key genes for the regulation of the autophagy pathway. In this study, our team has investigated the potential relationship between ATG5 gene polymorphism rs2245214 with non–small cell lung cancer (NSCLC) in a subpopulation of patients from southern Iran. In this study, 34 patients with NSCLC (20 males and 14 females [mean age: 12.86 ± 60.47 years]) and 50 healthy subjects (30 males and 20 females [mean age: 13.09 ± 56.62 years]) were studied in terms of the genotype of the ATG5 gene. We used restriction fragment length polymorphism and analyzed the results using SPSS software (v.23). The results revealed that subjects harboring the guanine/cytosine (GC) genotype of the rs2245214 ATG5 gene polymorphism had suffered less from NSCLC, whereas the prevalence of the C-allele of this polymorphism was significantly higher in patients with NSCLC ( P < 0.05). On the basis of the results of logistic regression, the presence of this C-allele may predict the risk of lung cancer ( P value = 0.011; OR, 3.52; 95% CI, 1.33-9.26). This study concludes that the C-allele of the rs2245214 ATG5 gene polymorphism is associated with increased susceptibility to NSCLC, whereas the GC genotype of this polymorphism is associated with decreased risk and might therefore have a protective role in the development of NSCLC. 相似文献
137.
F. Boufahja A. Hedfi J. Amorri P. Aïssa E. Mahmoudi H. Beyrem 《Journal of experimental marine biology and ecology》2011,399(2):142-150
Since 1981, many numerical indices have been developed for meiobenthic nematodes but later discarded. To overcome problems and drawbacks in this type of indices, the “relative volume of the pharyngeal lumen (RVPL)” was first introduced in biological assessments in 2006 and it focused on the assumption that pollution is commonly expressed in terms of reduced food intake. This index, evaluated by using a morphometric approach, expresses the sucking potential of the pharynx. A series of microcosm experiments was performed in order to test the “relative volume of the pharyngeal lumen (RVPL)” as a biomonitoring index in the cases of 8 free-living nematode species (the deposit-feeders, Daptonema normandicum, Desmodora longiseta, and Leptonemella aphanothecae; the epistrate feeder, Xyala striata; the facultative predators, Oncholaimus campylocercoides and Oncholaimellus mediterraneus; and the predators Bathylaimus capacosus and Mesacanthion hirsutum). Each species was exposed to various concentrations of toxicants (nickel, copper, chromium and Diesel Fuel) used separately or in combinations. Our results showed that RVPL increased with increasing amount of toxicants, visibly caused by a systematic decrease in body volume with toxicants. Responses of all examined species suggested an additive toxicological action. Predators (M. hirsutum and B. capacosus) showed the highest potential of adaptation because of their higher means of RVPL. Observations during this experiment highlight the importance of pharyngeal pumping and cuticular structure to tolerating elevated catabolic water loss under stress conditions. Thus the ability to osmoregulate is an important factor in overcoming food sedimentary toxicants. Based on all these findings, RVPL was found to be an accurate and precise index of monitoring the effect of metals and Diesel Fuel in marine ecosystems. 相似文献
138.
Enzymatic degradation of N-acyl homoserine lactone (NAHL) was found to interfere with the quorum sensing (QS) system and related functions in several soil bacteria. In this research, the NAHL lactonase gene aiiA was amplified using aiiA-7F/aiiA7R PCR primers from the quorum sensing inhibitor rhizobacterium Bacillus sp. strain DMS133, and cloned. The plasmid pME7075, carrying the DMS133 aiiA gene under the constitutive lac promoter, was introduced into the plant pathogen Pectobacterium carotovorum EMPCC, creating strain EMPCC/aiiA. Heterologous expression of the DMS133 aiiA gene in EMPCC severely reduced the accumulation of the NAHL throughout growth, and completely prevented pigmentation of the CV026 bioreporter strain. Virulence analysis revealed that the P. carotovorum strain EMPCC/aiiA expressing AiiA lactonase had drastically reduced tissue maceration activity compared with the wild type EMPCC strain. These results provide evidence that AiiA plays an important role in the quorum quenching ability of Bacillus sp. DMS133 whose AHL degradation capacity was investigated previously. In addition, the communication signal-inactivation approach represents a promising strategy for the prevention of diseases in which virulence is regulated by QS signal molecules. 相似文献
139.
Shaida Mahmoudi Mohamad Javad Chaichi Mojtaba Shamsipur Ome Leila Nazari Abdolraouf Samadi-Maybodi 《Luminescence》2023,38(10):1711-1719
Atropine (AT) is an anticholinergic drug. AT is abundantly in Datura plant seeds. Fe3O4@Zn/Mg MOF (Fe3O4@MOF) composite was synthesized. The compound had a high peroxidase-like activity in a chemiluminescence (CL) reaction. Addition of AT quenched CL. The linear range and limit of detection were 5–600 μg L−1 and 2 × 10−2 μg L−1. This method is fast, reversible, and selective, without biodegradability effects, high accuracy, and precision for measuring AT in the Datura plant. 相似文献
140.
Individual and combined effects of lead and zinc on a free-living marine nematode community: Results from microcosm experiments 总被引:2,自引:0,他引:2
E. Mahmoudi N. Essid A. Hedfi P. Vitiello 《Journal of experimental marine biology and ecology》2007,343(2):217-226
A microcosm experiment was carried out to study the influence of lead and zinc, individually and in mixtures, on a free-living nematode community of a Tunisian lagoon. Three levels (low, medium and high) of each heavy metal were used, separately and in combinations, for sediment contamination and effects were examined after two months. Results from the multiple comparison tests showed significant differences between nematode assemblages from undisturbed controls and those from lead and/or zinc treatments. Most univariate measures, including diversity and species richness, decreased significantly in the treated microcosms. Results from multivariate analyses of the species abundance data revealed significant effects of both metals, separately or in mixtures, at all the doses tested on nematode assemblages. All treatments were significantly different from the control and from each other. Both univariate and multivariate analyses of the data showed that the differential response occurred in all treatments but the communities from microcosms contaminated with lead and zinc separately were much more strongly affected. This result is suggestive of antagonistic interactions between Pb and Zn. The responses of nematode species to the lead and zinc treatments were varied: Calomicrolaimus honestus was eliminated at all lead doses tested and seemed to be intolerant species to Pb contamination whereas Oncholaimus campylocercoides increased significantly at low and medium lead contamination, was eliminated in Pb-highly amended sediment treatments and increased at all zinc doses used. This species could be categorized as “opportunistic” at low and medium lead doses and “zinc-resistant” species. Hypodontolaimus colesi was eliminated in all replicates treated with Zn even at low concentration and appeared to be a very sensitive species to zinc contamination. 相似文献