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121.
Siva Chennareddy Toby Cicak Tejinder Mall Katherine Effinger Nagesh Sardesai Dayakar Pareddy Rodrigo Sarria 《Plant Cell, Tissue and Organ Culture》2018,135(1):23-35
An improved direct transformation method via particle bombardment of split-immature zygotic embryo explants with intact embryonic axis is reported. This method involves abiotic stress (cold treatment and plasmolysis) treatments of explants prepared from immature embryos of 8–10 mm size for improved somatic embryogeneis. Transgenic events were produced using optimized bombardment conditions and selection with hygromycin or glufosinate. Transgenic somatic embryos developed within as little as 4 weeks after bombardment of explants. Transgenic plants were regenerated 4–5 months after bombardment and the entire process from bombardment to T1 seed production took 7–9 months. Plants regenerated from the system were fertile and showed more than 90% heritability of the transgene to the next generation. Transformation frequencies of 5.4 and 2.7% (based on the number of bombarded split-immature embryo explants) were observed with hygromycin and glufosinate selection, respectively. 相似文献
122.
Expression of fragile sites in childhood acute lymphoblastic leukemia patients and normal controls 总被引:1,自引:0,他引:1
Summary A high concordance has been reported between fragile sites and breakpoints involved in chromosomal rearrangements in cancer. A prospective study on the role of fragile sites in the etiology of childhood acute lymphocytic leukemia (ALL), with appropriate comparisons to results obtained from normal controls, analyzed fluorodeoxyuridine-, aphidicolin-, and caffeine-induced fragile sites in the peripheral blood of seven ALL patients (three with cytogenetically normal karyotype and four with pseudodiploid karyotype) and eight normal controls. While extensive variations in the number and distribution of fragile sites was observed within each group, there was no significant difference in the mean total fragile sites and mean fragile sites per cell between the two groups (P>0.05) in all three treatments. Similarly, within the ALL patients, the two karyotypic groups did not exhibit any significant difference in fragility (P>0.05). 相似文献
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Yepuri Vijay Jalali Saakshi Kancharla Nagesh Reddy V. B. Arockiasamy S. 《Molecular biology reports》2020,47(7):5091-5099
Molecular Biology Reports - Jatropha curcas is a potential biodiesel crop and a highly adaptable species to various agro-climatic conditions. In this study, we have utilized transposable... 相似文献
126.
Plasma gonadotropin, prolactin levels and hypothalamic tyrosine hydroxylase activity were evaluated at 0900, 1200 and 1700
h during diestrus, proestrus and estrus, ovariectomized and after systemic administration of reserpine or α-methyl p-tyrosine,
which interfere with catecholamine biosynthesis, in rats. Gonadotropin and prolactin levels showed peak values during the
afternoon of proestrus, while hypothalamic tyrosine hydroxylase activity was markedly lowered at 1200 on proestrus. Gonadotropin
levels were slightly lowered whereas prolactin concentrations and hypothalamic tyrosine hydroxylase activity were significantly
increased by reserpine. Depletion of hypothalamic dopamine by reserpine apparently resulted in significant elevation of prolactin
levels which inturn induce tyrosine hydroxylase. Gonadotropin levels and hypothalamic tyrosine hydroxylase activity were significantly
suppressed after the administration of α-methyl p-tyrosine. Prolactin levels, however, were elevated significantly. These
results indicate that catecholamines are involved in the control of gonadotropin and prolactin release during estrous cycle
and inhibition of catecholamines biosynthesis by α-methyl p-tyrosine could result in suppression of gonadotropin levels, whereas
removal of tonic inhibition of hypothalamic dopamine by α-methyl-p-tyrosine elevate prolactin levels. 相似文献