全文获取类型
收费全文 | 526篇 |
免费 | 45篇 |
出版年
2023年 | 2篇 |
2022年 | 3篇 |
2021年 | 16篇 |
2020年 | 8篇 |
2019年 | 8篇 |
2018年 | 17篇 |
2017年 | 12篇 |
2016年 | 8篇 |
2015年 | 23篇 |
2014年 | 27篇 |
2013年 | 40篇 |
2012年 | 41篇 |
2011年 | 37篇 |
2010年 | 27篇 |
2009年 | 20篇 |
2008年 | 27篇 |
2007年 | 28篇 |
2006年 | 29篇 |
2005年 | 21篇 |
2004年 | 25篇 |
2003年 | 14篇 |
2002年 | 18篇 |
2001年 | 14篇 |
2000年 | 13篇 |
1999年 | 10篇 |
1998年 | 2篇 |
1996年 | 4篇 |
1995年 | 2篇 |
1993年 | 5篇 |
1992年 | 5篇 |
1991年 | 4篇 |
1990年 | 3篇 |
1989年 | 4篇 |
1988年 | 6篇 |
1987年 | 5篇 |
1984年 | 3篇 |
1982年 | 3篇 |
1980年 | 6篇 |
1979年 | 6篇 |
1977年 | 3篇 |
1976年 | 1篇 |
1974年 | 1篇 |
1973年 | 3篇 |
1972年 | 3篇 |
1971年 | 1篇 |
1970年 | 1篇 |
1969年 | 1篇 |
1968年 | 2篇 |
1966年 | 1篇 |
1964年 | 1篇 |
排序方式: 共有571条查询结果,搜索用时 639 毫秒
411.
The presence of arginase in rat fibrosarcoma not synthesizing urea, suggested that this enzyme may have additional functions.
Ornithine carbamoyl transferase, a key enzyme of the urea cycle was absent in this tissue, when compared to normal tissues,
lower amount of ornithine was found in the fibrosarcoma, but this tumour contained a higher level of proline. The radioactivity
present in L-[U-14C] arginine was incorporated into putrescine, spermidine, spermine, proline glutamate and glutamine suggesting that arginine
was a possible precursor and that arginase may have a role in the synthesis of these metabolites. 相似文献
412.
Nagarajan Muthukaman Sanjay Deshmukh Shital Tondlekar Macchindra Tambe Dnyandeo Pisal Neelam Sarode Siddharth Mhatre Samitabh Chakraborti Daisy Shah Vikram M. Bhosale Abhay Kulkarni Mahamad Yunnus A. Mahat Satyawan B. Jadhav Girish S. Gudi Neelima Khairatkar-Joshi Laxmikant A. Gharat 《Bioorganic & medicinal chemistry letters》2018,28(23-24):3766-3773
Endogenous nitrosothiols (SNOs) including S-nitrosoglutathione (GSNO) serve as reservoir for bioavailable nitric oxide (NO) and mediate NO-based signaling, inflammatory status and smooth muscle function in the lung. GSNOR inhibition increases pulmonary GSNO and induces bronchodilation while reducing inflammation in lung diseases. In this letter, design, synthesis and structure–activity relationships (SAR) of novel imidazole-biaryl-tetrazole based GSNOR inhibitors are described. Many potent inhibitors (30, 39, 41, 42, 44, 45 and 58) were identified with low nanomolar activity (IC50s: <15?nM) along with adequate metabolic stability. Lead compounds 30 and 58 exhibited good exposure and oral bioavailability in mouse pharmacokinetic (PK) study. Compound 30 was selected for further profiling and revealed comparable mouse and rat GSNOR potency, high selectivity against alcohol dehydrogenase (ADH) and carbonyl reductase (CBR1) family of enzymes, low efflux ratio and permeability in PAMPA, a high permeability in CALU-3 assay, significantly low hERG activity and minimal off-target activity. Further, an in vivo efficacy of compound 30 is disclosed in cigarette smoke (CS) induced mouse model for COPD. 相似文献
413.
Parathyroid hormone‐induced down‐regulation of miR‐532‐5p for matrix metalloproteinase‐13 expression in rat osteoblasts
下载免费PDF全文
![点击此处可从《Journal of cellular biochemistry》网站下载免费的PDF全文](/ch/ext_images/free.gif)
414.
415.
416.
417.
Highly pathogenic avian influenza H5N1 virus induces cytokine dysregulation with suppressed maturation of chicken monocyte‐derived dendritic cells
下载免费PDF全文
![点击此处可从《Microbiology and immunology》网站下载免费的PDF全文](/ch/ext_images/free.gif)
Semmannan Kalaiyarasu Manoj Kumar Dhanapal Senthil Kumar Sandeep Bhatia Sandeep Kumar Dash Sushant Bhat Rohit K. Khetan Shanmugasundaram Nagarajan 《Microbiology and immunology》2016,60(10):687-693
One of the major causes of death in highly pathogenic avian influenza virus (HPAIV) infection in chickens is acute induction of pro‐inflammatory cytokines (cytokine storm), which leads to severe pathology and acute mortality. DCs and respiratory tract macrophages are the major antigen presenting cells that are exposed to mucosal pathogens. We hypothesized that chicken DCs are a major target for induction of cytokine dysregulation by H5N1 HPAIV. It was found that infection of chicken peripheral blood monocyte‐derived dendritic cells (chMoDCs) with H5N1 HPAIV produces high titers of progeny virus with more rounding and cytotoxicity than with H9N2 LPAIV. Expression of maturation markers (CD40, CD80 and CD83) was weaker in both H5N1 and H9N2 groups than in a LPS control group. INF‐α, ‐β and ‐γ were significantly upregulated in the H5N1 group. Pro‐inflammatory cytokines (IL‐1β, TNF‐α and IL‐18) were highly upregulated in early mid (IL‐1), and late (IL‐6) phases of H5N1 virus infection. IL‐8 (CXCLi2) mRNA expression was significantly stronger in the H5N1 group from 6 hr of infection. TLR3, 7, 15 and 21 were upregulated 24 hr after infection by H5N1 virus compared with H9N2 virus, with maximum expression of TLR 3 mRNA. Similarly, greater H5N1 virus‐induced apoptotic cell death and cytotoxicity, as measured by terminal deoxynucleotidyl transferase‐mediated dUTP nick end labeling and lactate dehydrogenase assays, respectively, were found. Thus, both H5N1 and H9N2 viruses evade the host immune system by inducing impairment of chMoDCs maturation and enhancing cytokine dysregulation in H5N1 HPAIV‐infected cells. 相似文献
418.
419.
Industrial wastewater bioreactors: sources of novel microorganisms for biotechnology 总被引:3,自引:0,他引:3
Microorganisms exist in nature as members of complex, mixed communities. The microbial communities in industrial wastewater bioreactors can be used as model systems to study the evolution of new metabolic pathways in natural ecosystems. The evolution of microbial metabolic capability in these bioreactors is presumably analogous to phenomena that occur in natural ecosystems. The microorganisms in these bioreactors compete for different carbon sources and constantly have to evolve new metabolic capabilities for survival. Thus, industrial bioreactors should be a rich source of novel biocatalysts. 相似文献
420.