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Plant and Soil - Unravelling the factors shaping microbial community structure across plant holobiont is required to promote plant health and crop productivity. We compared microbial communities in... 相似文献
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Ido Laish Hila Katz Yael Sulayev Meytal Liberman Timna Naftali Fabiana Benjaminov Assaf Stein Yona Kitay-Cohen Tal Biron-Shental Fred Konikoff Aliza Amiel 《Gene》2013
Objective
Primary sclerosing cholangitis (PSC) is a chronic cholestatic disorder that involves inflammatory and fibrotic changes in the bile ducts. Up to 80% of patients have concomitant inflammatory bowel disease (IBD) with colitis. PSC patients are predisposed to develop hepatobiliary, colonic and other extrahepatic malignancies, probably related to inflammatory processes that might promote carcinogenesis. Telomerase is an enzyme complex that lengthens telomeres and has enhanced expression in numerous malignancies. In this study, we evaluated the TERC gene copy number, the proportion of cells in senescence and the amount of fragmentation in the senescent state.Methods
Fluorescence in situ hybridization (FISH) for the TERC gene was applied to lymphocytes retrieved from PSC (N = 19), colitis (N = 20) and healthy control patients (N = 20) to determine the TERC copy number. On the same FISH slides, cells stained with DAPI were also analyzed for senescence-associated heterochromatin foci (SAHF) status, including the number of cells with fragments and the number of SAHF fragments in each cell.Results
A higher TERC gene copy number was observed in cells from PSC patients compared to colitis and control group patients. It was also higher in the colitis than in the control group. Significantly more cells in the senescent state and more fragmentation in each cell were observed in the PSC group compared to colitis and control groups.Conclusion
The TERC gene copy number and the number of cells in the senescent state were increased in PSC patients compared to the colitis and control groups. These findings are probably related to the genetic instability parameters that reflect the higher tendency of this patient group to develop malignancies. 相似文献43.
Degu A Hatew B Nunes-Nesi A Shlizerman L Zur N Katz E Fernie AR Blumwald E Sadka A 《Planta》2011,234(3):501-513
Citrate, a major determinant of citrus fruit quality, accumulates early in fruit development and declines towards maturation.
The isomerization of citrate to isocitrate, catalyzed by aconitase is a key step in acid metabolism. Inhibition of mitochondrial
aconitase activity early in fruit development contributes to acid accumulation, whereas increased cytosolic activity of aconitase
causes citrate decline. It was previously hypothesized that the block in mitochondrial aconitase activity, inducing acid accumulation,
is caused by citramalate. Here, we investigated the effect of citramalate and of another aconitase inhibitor, oxalomalate,
on aconitase activity and regulation in callus originated from juice sacs. These compounds significantly increased citrate
content and reduced the enzyme’s activity, while slightly inducing its protein level. Citramalate inhibited the mitochondrial,
but not cytosolic form of the enzyme. Its external application to mandarin fruits resulted in inhibition of aconitase activity,
with a transient increase in fruit acidity detected a few weeks later. The endogenous level of citramalate was analyzed in
five citrus varieties: its pattern of accumulation challenged the notion of its action as an endogenous inhibitor of mitochondrial
aconitase. Metabolite profiling of oxalomalate-treated cells showed significant increases in a few amino acids and organic
acids. The activities of alanine transaminase, aspartate transaminase and aspartate kinase, as well as these of two γ-aminobutyrate
(GABA)-shunt enzymes, succinic semialdehyde reductase (SSAR) and succinic semialdehyde dehydrogenase (SSAD) were significantly
induced in oxalomalate-treated cells. It is suggested that the increase in citrate, caused by aconitase inhibition, induces
amino acid synthesis and the GABA shunt, in accordance with the suggested fate of citrate during the acid decline stage in
citrus fruit. 相似文献
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Louis J. Vuga Jadranka Milosevic Kusum Pandit Ahmi Ben-Yehudah Yanxia Chu Thomas Richards Joshua Sciurba Michael Myerburg Yingze Zhang Anil V. Parwani Kevin F. Gibson Naftali Kaminski 《PloS one》2013,8(12)
Idiopathic pulmonary fibrosis (IPF) is a progressive and life threatening disease with median survival of 2.5–3 years. The IPF lung is characterized by abnormal lung remodeling, epithelial cell hyperplasia, myofibroblast foci formation, and extracellular matrix deposition. Analysis of gene expression microarray data revealed that cartilage oligomeric matrix protein (COMP), a non-collagenous extracellular matrix protein is among the most significantly up-regulated genes (Fold change 13, p-value <0.05) in IPF lungs. This finding was confirmed at the mRNA level by nCounter® expression analysis in additional 115 IPF lungs and 154 control lungs as well as at the protein level by western blot analysis. Immunohistochemical analysis revealed that COMP was expressed in dense fibrotic regions of IPF lungs and co-localized with vimentin and around pSMAD3 expressing cells. Stimulation of normal human lung fibroblasts with TGF-β1 induced an increase in COMP mRNA and protein expression. Silencing COMP in normal human lung fibroblasts significantly inhibited cell proliferation and negatively impacted the effects of TGF-β1 on COL1A1 and PAI1. COMP protein concentration measured by ELISA assay was significantly increased in serum of IPF patients compared to controls. Analysis of serum COMP concentrations in 23 patients who had prospective blood draws revealed that COMP levels increased in a time dependent fashion and correlated with declines in force vital capacity (FVC). Taken together, our results should encourage more research into the potential use of COMP as a biomarker for disease activity and TGF-β1 activity in patients with IPF. Hence, studies that explore modalities that affect COMP expression, alleviate extracellular matrix rigidity and lung restriction in IPF and interfere with the amplification of TGF-β1 signaling should be persuaded. 相似文献
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The HLA class II Allele DRB1*1501 is over-represented in patients with idiopathic pulmonary fibrosis
Xue J Gochuico BR Alawad AS Feghali-Bostwick CA Noth I Nathan SD Rosen GD Rosas IO Dacic S Ocak I Fuhrman CR Cuenco KT Smith MA Jacobs SS Zeevi A Morel PA Pilewski JM Valentine VG Gibson KF Kaminski N Sciurba FC Zhang Y Duncan SR 《PloS one》2011,6(2):e14715
Background
Idiopathic pulmonary fibrosis (IPF) is a progressive and medically refractory lung disease with a grim prognosis. Although the etiology of IPF remains perplexing, abnormal adaptive immune responses are evident in many afflicted patients. We hypothesized that perturbations of human leukocyte antigen (HLA) allele frequencies, which are often seen among patients with immunologic diseases, may also be present in IPF patients.Methods/Principal Findings
HLA alleles were determined in subpopulations of IPF and normal subjects using molecular typing methods. HLA-DRB1*15 was over-represented in a discovery cohort of 79 Caucasian IPF subjects who had lung transplantations at the University of Pittsburgh (36.7%) compared to normal reference populations. These findings were prospectively replicated in a validation cohort of 196 additional IPF subjects from four other U.S. medical centers that included both ambulatory patients and lung transplantation recipients. High-resolution typing was used to further define specific HLA-DRB1*15 alleles. DRB1*1501 prevalence in IPF subjects was similar among the 143 ambulatory patients and 132 transplant recipients (31.5% and 34.8%, respectively, p = 0.55). The aggregate prevalence of DRB1*1501 in IPF patients was significantly greater than among 285 healthy controls (33.1% vs. 20.0%, respectively, OR 2.0; 95%CI 1.3–2.9, p = 0.0004). IPF patients with DRB1*1501 (n = 91) tended to have decreased diffusing capacities for carbon monoxide (DLCO) compared to the 184 disease subjects who lacked this allele (37.8±1.7% vs. 42.8±1.4%, p = 0.036).Conclusions/Significance
DRB1*1501 is more prevalent among IPF patients than normal subjects, and may be associated with greater impairment of gas exchange. These data are novel evidence that immunogenetic processes can play a role in the susceptibility to and/or manifestations of IPF. Findings here of a disease association at the HLA-DR locus have broad pathogenic implications, illustrate a specific chromosomal area for incremental, targeted genomic study, and may identify a distinct clinical phenotype among patients with this enigmatic, morbid lung disease. 相似文献47.
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Dalia Somjen Fortune Kohen Batya Gayer Esther Knoll Ariel Many Naftali Stern 《The Journal of steroid biochemistry and molecular biology》2009,113(3-5):171-176
We reported previously that high concentrations of either estradiol-17β (E2) or dihydrotestosterone (DHT) inhibit growth of human cultured vascular smooth muscle cells (VSMC), mediated by cell membrane receptors and MAP-kinase–kinase activity (MEK). We now tested whether the presence of the opposite gender's dominant sex hormone modifies these effects. We incubated VSMC with various concentrations of E2 and DHT or protein bound hormones (E2–BSA or T–BSA), alone or in various combinations. High concentration of E2 or E2–BSA inhibited VSMC growth and stimulated MEK. In the presence of 3 nM DHT, high concentration of E2 no longer inhibited 3[H] thymidine incorporation or increased MEK. Moreover, when high DHT concentration (300 nM) was added to VSMC exposed to high E2, VSMC growth actually increased without change in MEK. DHT at 300 nM suppressed VSMC growth and increased MEK while 0.3 nM E2 had only marginal effect on this interaction, and 30 nM E2 reversed the inhibitory effect of DHT on cell growth. The inhibitory effects of both E2 and DHT on VSMC cell growth and the stimulation of MEK was apparently mediated by cell membrane receptors, as it persisted when bovine serum albumin (BSA)-bound hormones were used. Further, inhibition of VSMC growth induced by E2–BSA was reversed in the presence of T–BSA and vice versa. These results suggest that while female and male sex hormones affect VSMC growth similarly, they interfere in a dose-, hormone- and MEK-dependent manner with each other's effect. 相似文献
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