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271.
272.
Production and evaluation of novel formulations of tizanidine and tramadol microparticles was the chief purpose of this project. Microparticles of both drugs were prepared separately via temperature change method. To extend the release of formulations, ethyl cellulose was employed. Higuchi, zero-order, first-order, and Korsmeyer–Peppas kinetic models were applied to appraise the mechanism and mode of drugs release. Higuichi model was found to be best for all release profiles. Stability of microparticles at 40°C/75% RH over a 3-month duration was determined by Fourier transform infrared (FTIR), X-ray diffractometry (XRD), and drugs assay. Microparticles were compatible and stable as no significant differences were observed when subjected to drug assay, FTIR, and XRD during accelerated stability studies. 相似文献
273.
Muhammad Arshad Rafiq Andreas W. Kuss Lucia Puettmann Abdul Noor Annapoorani Ramiah Ghazanfar Ali Hao Hu Nadir Ali Kerio Yong Xiang Masoud Garshasbi Muzammil Ahmad Khan Gisele E. Ishak Rosanna Weksberg Reinhard Ullmann Andreas Tzschach Kimia Kahrizi Khalid Mahmood Farooq Naeem Muhammad Ayub Kelley W. Moremen John B. Vincent Hans Hilger Ropers Muhammad Ansar Hossein Najmabadi 《American journal of human genetics》2011,89(1):176-182
We have used genome-wide genotyping to identify an overlapping homozygosity-by-descent locus on chromosome 9q34.3 (MRT15) in four consanguineous families affected by nonsyndromic autosomal-recessive intellectual disability (NS-ARID) and one in which the patients show additional clinical features. Four of the families are from Pakistan, and one is from Iran. Using a combination of next-generation sequencing and Sanger sequencing, we have identified mutations in the gene MAN1B1, encoding a mannosyl oligosaccharide, alpha 1,2-mannosidase. In one Pakistani family, MR43, a homozygous nonsense mutation (RefSeq number : c.1418G>A [p.Trp473∗]), segregated with intellectual disability and additional dysmorphic features. We also identified the missense mutation c. 1189G>A (p.Glu397Lys; RefSeq number NM_016219.3), which segregates with NS-ARID in three families who come from the same village and probably have shared inheritance. In the Iranian family, the missense mutation c.1000C>T (p.Arg334Cys; RefSeq number NM_016219.3) also segregates with NS-ARID. Both missense mutations are at amino acid residues that are conserved across the animal kingdom, and they either reduce kcat by ∼1300-fold or disrupt stable protein expression in mammalian cells. MAN1B1 is one of the few NS-ARID genes with an elevated mutation frequency in patients with NS-ARID from different populations. NM_016219.3相似文献
274.
Ullah Najeeb Ling Xu Zammurad I. Ahmed Muhammad Rasheed Ghulam Jilani Muhammad S. Naeem Weiqi Shen Weijun Zhou 《Acta Physiologiae Plantarum》2011,33(2):481-488
Increased ultraviolet radiations intruding on the earth pose a serious threat to the unadapted plants. Due to the ecological
and economic significance of mat rush (Juncus effusus L.), an in vitro experiment was conducted to unveil the toxic effects of ultraviolet radiation (UV-C) on its physiology and
ultrastructure. The basal culm segments of plant were used for in vitro treatments of UV-C with different exposure times (15,
30 and 45 min). The treated segments were first transferred to 1/2 strength MS media and then shifted to soil-perlite mixture
for further growth. With increasing exposure time to UV-C, there was significant reduction in plant growth and biomass, and
increased activities of antioxidant enzymes. Physiological and ultrastructural alterations were observed in the shoots of
UV-treated plants. These plants exhibited significant reduction in chlorophyll contents and noticeable modifications at the
ultra-cellular levels. Cell and chloroplast size reduced greatly, and there was appearance of higher amounts of plastoglobuli
in chloroplasts resulting in disruption of thylakoid integrity. The functional and ultrastructural alterations in the stressed
plants suggest a potential hazard of UV-C radiation on this aquatic flora and thus the ecosystem. The study further explores
that UV-C radiations trigger these modifications mainly by damaging the chloroplast. 相似文献
275.
276.
Qazi Rida-e-Maria Naeem Nadia Khan Irfan Qadeer Quratulain Shaheen Farzana Salim Asmat 《Molecular and cellular biochemistry》2020,465(1-2):27-36
Molecular and Cellular Biochemistry - Polo-like kinase 1 (Plk1) is a mitotic serine/threonine kinase implicated in spindle formation and cytokinesis in mammalian cells. Here, purified Plk1 was... 相似文献
277.
Sanna Koskiniemi Henry S. Gibbons Linus Sandegren Naeem Anwar Gary Ouellette Stacey Broomall Mark Karavis Paul McGregor Alvin Liem Ed Fochler Lauren McNew Carolyn Nicole Rosenzweig Mikael Rhen Evan W. Skowronski Dan I. Andersson 《PloS one》2013,8(7)
How pathogenic bacteria adapt and evolve in the complex and variable environment of the host remains a largely unresolved question. Here we have used whole genome sequencing of Salmonella enterica serovar Typhimurium LT2 populations serially passaged in mice to identify mutations that adapt bacteria to systemic growth in mice. We found unique pathoadaptive mutations in two global regulators, phoQ and stpA, which increase the competitive indexes of the bacteria 3- to 5-fold. Also, all mouse-adapted lineages had changed the orientation of the hin invertable element, resulting in production of a FliC type of flagellum. Competition experiments in mice with locked flagellum mutants showed that strains expressing the FliC type of flagellum had a 5-fold increase in competitive index as compared to those expressing FljB type flagellum. Combination of the flagellum cassette inversion with the stpA mutation increased competitive indexes up to 20-fold. These experiments show that Salmonella can rapidly adapt to a mouse environment by acquiring a few mutations of moderate individual effect that when combined confer substantial increases in growth. 相似文献
278.
Yogesh S. Sanghvi Naeem B. Hanna Steven B. Larson Roland K. Robins Ganapathi R. Revankar 《Nucleosides, nucleotides & nucleic acids》2013,32(4):761-774
Abstract A synthesis of 1-(2,3-dideoxy-β-D-ribofuranosyl)-1,2,4-triazole-3-carboxamide (2′,3′-dideoxyribavirin, ddR) is described. Glycosylation of the sodium salt of 1,2,4-triazole-3-carbonitrile (5) with 1-chloro-2-deoxy-3,5-di-0-p-toluoyl-α-D-erythro-pentofuranose (1) gave exclusively the corresponding N-1 glycosyl derivative with β-anomeric configuration (6), which on ammonolysis provided a convenient synthesis of 2′-deoxyribavirin (7). Similar glycosylation of the sodium salt of methyl 1,2,4-triazole-3-carboxylate (2) with 1 gave a mixture of corresponding N-1 and N-2 glycosyl derivatives (3) and (4), respectively. Ammonolysis of 3 furnished yet another route to 7. A four-step deoxygenation procedure using imidazolylthiocarbonylation of the 3′-hydroxy group of 5′-0-toluoyl derivative (9a) gave ddR (11). The structure of 11 was proven by single crystal X-ray studies. In a preliminary in vitro study ddR was found to be inactive against HIV retrovirus. 相似文献
279.
Role of Salicylic Acid in Promoting Salt Stress Tolerance and Enhanced Artemisinin Production in Artemisia annua L. 总被引:1,自引:0,他引:1
Tariq Aftab M. Masroor A. Khan Jaime A. Teixeira da Silva Mohd. Idrees M. Naeem Moinuddin 《Journal of Plant Growth Regulation》2011,30(4):425-435
In the present investigation, the role of salicylic acid (SA) in inducing salinity tolerance was studied in Artemisia annua L., which is a major source of the antimalarial drug artemisinin. SA, when applied at 1.00 mM, provided considerable protection
against salt stress imposed by adding 50, 100, or 200 mM NaCl to soil. Salt stress negatively affected plant growth as assessed
by length and dry weight of shoots and roots. Salinity also reduced the values of photosynthetic attributes and total chlorophyll
content and inhibited the activities of nitrate reductase and carbonic anhydrase. Furthermore, salt stress significantly increased
electrolyte leakage and proline content. Salt stress also induced oxidative stress as indicated by the elevated levels of
lipid peroxidation compared to the control. A foliar spray of SA at 1.00 mM promoted the growth of plants, independent of
salinity level. The activity of antioxidant enzymes, namely, catalase, peroxidase, and superoxide dismutase, was upregulated
by salt stress and was further enhanced by SA treatment. Artemisinin content increased at 50 and 100 mM NaCl but decreased
at 200 mM NaCl. The application of SA further enhanced artemisinin content when applied with 50 and 100 mM NaCl by 18.3 and
52.4%, respectively. These results indicate that moderate saline conditions can be exploited to obtain higher artemisinin
content in A. annua plants, whereas the application of SA can be used to protect plant growth and induce its antioxidant defense system under
salt stress. 相似文献
280.