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21.
Wouter Coppieters Juliette Riquet Juan-José Arranz Paulette Berzi Nadine Cambisano Bernard Grisart Latifa Karim Fabienne Marcq Laurence Moreau Carine Nezer Patricia Simon Pascal Vanmanshoven Danny Wagenaar Michel Georges 《Mammalian genome》1998,9(7):540-544
A whole genome scan was undertaken in a granddaughter design comprising 1158 progeny-tested bulls in order to map QTL influencing
milk yield and composition. In this paper we report the identification of a locus on the centromeric end of bovine Chromosome
(Chr) 14, with major effect on fat and protein percentage as well as milk yield. The genuine nature of this QTL was verified
using the grand2-daughter design, that is, by tracing the segregating QTL alleles from heterozygous grandsires to their maternal grandsons
and confirming the predicted QTL allele substitution effect.
Received: 30 December 1997 / Accepted: 21 February 1998 相似文献
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Leishmania major LmACR2 is a pentavalent antimony reductase that confers sensitivity to the drug pentostam 总被引:4,自引:0,他引:4
Zhou Y Messier N Ouellette M Rosen BP Mukhopadhyay R 《The Journal of biological chemistry》2004,279(36):37445-37451
Arsenicals and antimonials are first line drugs for the treatment of trypanosomal and leishmanial diseases. To create the active form of the drug, Sb(V) must be reduced to Sb(III). Because arsenic and antimony are related metalloids, and arsenical resistant Leishmania strains are frequently cross-resistant to antimonials, we considered the possibility that Sb(V) is reduced by a leishmanial As(V) reductase. The sequence for the arsenate reductase of Saccharomyces cerevisiae, ScAcr2p, was used to clone the gene for a homologue, LmACR2, from Leishmania major. LmACR2 was able to complement the arsenate-sensitive phenotype of an arsC deletion strain of Escherichia coli or an ScACR2 deletion strain of Saccharomyces cerevisiae. Transfection of Leishmania infantum with LmACR2 augmented Pentostam sensitivity in intracellular amastigotes. LmACR2 was purified and shown to reduce both As(V) and Sb(V). This is the first report of an enzyme that confers Pentostam sensitivity in intracellular amastigotes of Leishmania. We propose that LmACR2 is responsible for reduction of the pentavalent antimony in Pentostam to the active trivalent form of the drug in Leishmania. 相似文献
23.
An expert system model for mapping tropical wetlands and peatlands reveals South America as the largest contributor 下载免费PDF全文
Thomas Gumbricht Rosa Maria Roman‐Cuesta Louis Verchot Martin Herold Florian Wittmann Ethan Householder Nadine Herold Daniel Murdiyarso 《Global Change Biology》2017,23(9):3581-3599
Wetlands are important providers of ecosystem services and key regulators of climate change. They positively contribute to global warming through their greenhouse gas emissions, and negatively through the accumulation of organic material in histosols, particularly in peatlands. Our understanding of wetlands’ services is currently constrained by limited knowledge on their distribution, extent, volume, interannual flood variability and disturbance levels. We present an expert system approach to estimate wetland and peatland areas, depths and volumes, which relies on three biophysical indices related to wetland and peat formation: (1) long‐term water supply exceeding atmospheric water demand; (2) annually or seasonally water‐logged soils; and (3) a geomorphological position where water is supplied and retained. Tropical and subtropical wetlands estimates reach 4.7 million km2 (Mkm2). In line with current understanding, the American continent is the major contributor (45%), and Brazil, with its Amazonian interfluvial region, contains the largest tropical wetland area (800,720 km2). Our model suggests, however, unprecedented extents and volumes of peatland in the tropics (1.7 Mkm2 and 7,268 (6,076–7,368) km3), which more than threefold current estimates. Unlike current understanding, our estimates suggest that South America and not Asia contributes the most to tropical peatland area and volume (ca. 44% for both) partly related to some yet unaccounted extended deep deposits but mainly to extended but shallow peat in the Amazon Basin. Brazil leads the peatland area and volume contribution. Asia hosts 38% of both tropical peat area and volume with Indonesia as the main regional contributor and still the holder of the deepest and most extended peat areas in the tropics. Africa hosts more peat than previously reported but climatic and topographic contexts leave it as the least peat‐forming continent. Our results suggest large biases in our current understanding of the distribution, area and volumes of tropical peat and their continental contributions. 相似文献
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Gaurav S. Kandlikar Christopher A. Johnson Xinyi Yan Nathan J. B. Kraft Jonathan M. Levine 《Ecology letters》2019,22(8):1178-1191
Interactions between plants and soil microbes can strongly influence plant diversity and community dynamics. Soil microbes may promote plant diversity by driving negative frequency‐dependent plant population dynamics, or may favor species exclusion by providing one species an average fitness advantage over others. However, past empirical research has focused overwhelmingly on the consequences of frequency‐dependent feedbacks for plant species coexistence and has generally neglected the consequences of microbially mediated average fitness differences. Here we use theory to develop metrics that quantify microbially mediated plant fitness differences, and show that accounting for these effects can profoundly change our understanding of how microbes influence plant diversity. We show that soil microbes can generate fitness differences that favour plant species exclusion when they disproportionately harm (or favour) one plant species over another, but these fitness differences may also favor coexistence if they trade off with competition for other resources or generate intransitive dominance hierarchies among plants. We also show how the metrics we present can quantify microbially mediated fitness differences in empirical studies, and explore how microbial control over coexistence varies along productivity gradients. In all, our analysis provides a more complete theoretical foundation for understanding how plant–microbe interactions influence plant diversity. 相似文献
26.
Nitrous oxide and methane fluxes of a pristine slope mire in the German National Park Harz Mountains
Nadine Tauchnitz Rainer Brumme Sabine Bernsdorf Ralph Meissner 《Plant and Soil》2008,303(1-2):131-138
Pristine peatlands covered by Histosols (bogs and fens) with high water table and a restricted oxygen (O2) availability are known to have low emissions of nitrous oxide (N2O) but may be a significant source for atmospheric methane (CH4) which are both important greenhouse gases. For the first time N2O and CH4 fluxes of a pristine slope mire in the German Harz Mountains have been monitored. Previously reported peatlands are characterised
by anaerobic conditions due to high water table levels. Slope mires monitored here receive O2 through slope water inflow. Gas fluxes have been monitored deploying closed chamber method on a central non-forested area
and a forested area at the periphery of the slope mire. By means of groundwater piezometers water table levels, ammonium and
nitrate contents as well as hydro-chemical variables like oxygen content and redox potential of the mire pore water have been
concurrently measured with trace gas fluxes at both monitoring sites of the slope mire. The slope mire took up small amounts
of atmospheric methane at a rate of −0.02 ± 0.01 kg C ha−1 year−1 revealing no significant difference between the forested and non-forested site. Higher uptake rates were observed during
low water table level. In contrast to pristine peatlands influx of oxygen containing pore water into slope mire does limit
reduction processes and resultant CH4 emission. N2O fluxes of the forested and non-forested sites of the slope mire did not differ and amounted to 0.25 ± 0.44 kg N ha−1 year−1. Higher emissions were observed at low water table levels and during thawing periods. In spite of favourable conditions N2O fluxes of the slope mire have been comparable to those of pristine peatlands. 相似文献
27.
Michael R D'Andrea Yuhong Qiu Donna Haynes-Johnson Sheela Bhattacharjee Patricia Kraft Scott Lundeen 《The journal of histochemistry and cytochemistry》2005,53(7):895-903
Cyclic nucleotide phosphodiesterase 11A (PDE11A) is the newest member in the PDE family. Although the tissue distribution of PDE11A mRNA has been shown, its protein expression pattern has not been well studied. The goal of this report is to investigate the distribution of PDE11A proteins in a wide range of normal and malignant human tissues. We utilized a polyclonal antibody that recognized all four PDE11A isoforms. Its specificity was demonstrated by Western blot analysis on a recombinant human PDE11A protein and native PDE11A proteins in various human tissues. Immunohistochemistry showed that PDE11A is widely expressed. Various degrees of immunoreactivity were observed in the epithelial cells, endothelial cells, and smooth muscle cells of all tissues examined. The highest expression was in the epithelial, endothelial, and smooth muscle cells of the prostate, Leydig, and spermatogenic cells of the testis, the tubule epithelial cells in the kidney, the epithelial and endothelial cells in the adrenal, the epithelial cells and macrophages in the colon, and the epidermis in the skin. Furthermore, PDE11A expression was also detected in several human carcinomas. Our results suggest that PDE11A might be involved in multiple physiological processes in various organs via its ability to modulate intracellular cAMP and cGMP levels. 相似文献
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