全文获取类型
收费全文 | 156篇 |
免费 | 12篇 |
国内免费 | 1篇 |
出版年
2023年 | 2篇 |
2022年 | 4篇 |
2021年 | 8篇 |
2020年 | 5篇 |
2019年 | 11篇 |
2018年 | 9篇 |
2017年 | 6篇 |
2016年 | 7篇 |
2015年 | 6篇 |
2014年 | 6篇 |
2013年 | 11篇 |
2012年 | 8篇 |
2011年 | 13篇 |
2010年 | 8篇 |
2009年 | 9篇 |
2008年 | 6篇 |
2007年 | 5篇 |
2006年 | 5篇 |
2005年 | 5篇 |
2004年 | 8篇 |
2003年 | 3篇 |
2002年 | 3篇 |
2001年 | 1篇 |
2000年 | 1篇 |
1999年 | 3篇 |
1998年 | 4篇 |
1997年 | 2篇 |
1995年 | 2篇 |
1991年 | 1篇 |
1990年 | 1篇 |
1989年 | 1篇 |
1986年 | 1篇 |
1983年 | 2篇 |
1979年 | 1篇 |
1971年 | 1篇 |
排序方式: 共有169条查询结果,搜索用时 15 毫秒
81.
Cannabinoid system plays an important role in controlling neuronal excitability and brain function. On the other hand, modulation
of gamma-aminobutyric acid (GABA) transmission is one of the initial strategies for the treatment of seizure. The aim of the
present study was to evaluate possible interaction between cannabinoidergic and GABAergic systems in pentylenetetrazole (PTZ)-induced
acute seizure in rat. Drugs were administered by intracerebroventricular (i.c.v.) administration 20 min before a single intraperitoneal
(i.p.) injection of PTZ and the latency to the first generalized tonic-clonic seizure was measured. Both the cannabinoid receptor
agonist WIN55212-2 (10, 30, 50 and 100 μg/rat) and the GABA-A receptor agonist isoguvacine (IGN; 10, 30 and 50 μg/rat) significantly
increased the latency of seizure occurrence. Moreover, the fatty acid amide hydrolase inhibitor URB597 showed no anticonvulsive
effect while the monoacyl glycerol lipase (MAGL) inhibitor URB602 (10, 50 and 100 μg/rat) protected rats against PTZ-induced
seizure. Moreover, co-administration of IGN and cannabinoid compounds attenuated the anticonvulsant action of both WIN55212-2
and IGN in this model of seizure. Our data suggests that exogenous cannabinoid WIN55212-2 and MAGL inhibitor URB602 imply
their antiseizure action in part through common brain receptorial system. Moreover, the antagonistic interaction of cannabinoids
and IGN in protection against PTZ-induced seizure could suggest the involvement of GABAergic system in their anticonvulsant
action. 相似文献
82.
Proteomics analysis of chinese hamster ovary cells undergoing apoptosis during prolonged cultivation
Wei YY Naderi S Meshram M Budman H Scharer JM Ingalls BP McConkey BJ 《Cytotechnology》2011,63(6):663-677
The degradation of environmental conditions, such as nutrient depletion and accumulation of toxic waste products over time,
often lead to premature apoptotic cell death in mammalian cell cultures and suboptimal protein yield. Although apoptosis has
been extensively researched, the changes in the whole cell proteome during prolonged cultivation, where apoptosis is a major
mode of cell death, have not been examined. To our knowledge, the work presented here is the first whole cell proteome analysis
of non-induced apoptosis in mammalian cells. Flow cytometry analyses of various activated caspases demonstrated the onset
of apoptosis in Chinese hamster ovary cells during prolonged cultivation was primarily through the intrinsic pathway. Differential
in gel electrophoresis proteomic study comparing protein samples collected during cultivation resulted in the identification
of 40 differentially expressed proteins, including four cytoskeletal proteins, ten chaperone and folding proteins, seven metabolic
enzymes and seven other proteins of varied functions. The induction of seven ER chaperones and foldases is a solid indication
of the onset of the unfolded protein response, which is triggered by cellular and ER stresses, many of which occur during
prolonged batch cultures. In addition, the upregulation of six glycolytic enzymes and another metabolic protein emphasizes
that a change in the energy metabolism likely occurred as culture conditions degraded and apoptosis advanced. By identifying
the intracellular changes during cultivation, this study provides a foundation for optimizing cell line-specific cultivation
processes, prolonging longevity and maximizing protein production. 相似文献
83.
Gheisari Y Ahmadbeigi N Naderi M Nassiri SM Nadri S Soleimani M 《Cell biology international》2011,35(3):209-213
Paracrine secretion of mediators may be the main route by which stem cells protect against injuries. Stem cells commonly secrete different bioactive molecules. In this study, we examined the hypothesis that administration of conditioned media of stem cells can diminish the burden of kidney injury. A mouse model of cisplatin-induced nephropathy was developed to test the putative renoprotective effects of conditioned media of human umbilical cord blood USSCs (unrestricted somatic stem cells) as well as mouse bone marrow MSCs (mesenchymal stem cells). None of these two types of conditioned medium could protect against kidney failure in terms of serum urea and creatinine, histopathologic examinations and physical activity score. Neither MSC- nor USSC-conditioned media were effective in protecting against kidney injury in our study. Possible explanations for our observations are offered, and related literature is reviewed. 相似文献
84.
Reina JA de Kinderen Danielle AJE Lambrechts Debby Postulart Alfons GH Kessels Jos GM Hendriksen Albert P Aldenkamp Marian HJM Majoie 《BMC neurology》2011,11(1):10
Background
Epilepsy is a neurological disorder, characterized by recurrent unprovoked seizures which have a high impact on the individual as well as on society as a whole. In addition to the economic burden, epilepsy imposes a substantial burden on the patients and their surroundings. Patients with uncontrolled epilepsy depend heavily on informal care and on health care professionals. About 30% of patients suffer from drug-resistant epilepsy. The ketogenic diet can be a treatment of last resort, especially for children. The beneficial effect of the ketogenic diet has been proven, but information is lacking about its cost-effectiveness. In the current study we will evaluate the (cost-) effectiveness of the ketogenic diet in children and adolescents with intractable epilepsy. 相似文献85.
Autophagy plays an important role in cellular remodelling during differentiation and development, however little is known about its regulation in stem cells. Here we show that cAMP, a well-known differentiation factor for mesenchymal stem cells (MSCs), is also a potent inducer of autophagy in these cells. We have previously shown that activation of the cAMP-signaling pathway inhibits proliferation of MSCs despite induction of the cell cycle component cyclin E. Here, we demonstrate a critical role of cyclin E in the induction of autophagy. Our data suggest a model in which cAMP-signaling via ERK-mediated induction of cyclin E leads to enhanced perinuclear recruitment of Beclin 1 and formation of autophagosomes. Given the roles of deregulated autophagy in neurodegenerative disorders and cAMP as a neurogenic inducer, identification of this novel autophagocytic pathway may provide new targets for intervention against neurological disorders. 相似文献
86.
The age- and cytomegalovirus (CMV)-seropositivity-related changes in subsets and differentiation of circulating T cells were investigated in end-stage renal disease (ESRD) patients (n = 139) and age-matched healthy individuals. The results show that CMV-seropositivity is associated with expansion of both CD4+ and CD8+ memory T cells which is already observed in young healthy individuals. In addition, CMV-seropositive healthy individuals have a more differentiated memory T cell profile. Only CMV-seropositive healthy individuals showed an age-dependent decrease in CD4+ naïve T cells. The age-related decrease in the number of CD8+ naïve T cells was CMV-independent. In contrast, all ESRD patients showed a profound naïve T-cell lymphopenia at every decade. CMV-seropositivity aggravated the contraction of CD4+ naïve T cells and increased the number of differentiated CD4+ and CD8+ memory T cells. In conclusion, CMV-seropositivity markedly alters the homeostasis of circulating T cells in healthy individuals and aggravates the T cell dysregulation observed in ESRD patients. 相似文献
87.
88.
89.
Akram Sadeghi Ali reza Hesan Hossein Askari Davood Naderi Qomi Maryam Farsi Eslam Majidi Hervan 《Biocontrol Science and Technology》2009,19(9):985-991
A 3-year study was conducted to evaluate the effectiveness of two disease-suppressive Streptomyces spp. to control sugar beet Rhizoctonia solani damping off under field conditions. Streptomyces seed treatments reduced seedling damping off in naturally (2005) and artificially (2006 and 2007) infested soils. All biocontrol agents provided better efficacy than Vitavax to control seedling damping-off. There were no significant differences among Streptomyces isolates. Isolate C increased plant stand by 19.5, 50.5 and 53.75% in 2005, 2006 and 2007, respectively. Evaluation of final harvest revealed that the root yield of the biocontrol agents increased compared to untreated control in these years. 相似文献
90.
Hamid Galehdari Ali Mohammad Foroughmand Maryam Naderi Soorki Gholamreza Mohammadian 《Indian journal of human genetics》2009,15(1):9-12