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MARIE‐CLAUDE BEL‐VENNER NATHALIE MONDY FLORENT ARTHAUD JULIEN MARANDET DAVID GIRON SAMUEL VENNER FREDERIC MENU 《Physiological Entomology》2009,34(1):61-70
Abstract Diapausing insect species have evolved a great diversity of life cycles, although overwintering occurs at a single development stage within most species. Understanding why diapause has evolved towards a given life stage requires investigation of both the ecological and physiological attributes. Notably, it is suggested that adult overwintering is more energy‐demanding than larval overwintering but it brings fitness gains by allowing adults to be synchronized with their seasonal requisites through an early spring emergence. This hypothesis is tested in field conditions in the nut weevil Curculio nucum, whose life cycle comprises an obligate 2‐year, nonfeeding underground phase, including a larval, followed by an adult, overwintering. In this species, adult wintering leads to an early spring emergence; at first glance, however, this does not enhance synchronization between weevils and their host because adults emerge more than 1 month before starting to breed. It is suggested that adult overwintering ultimately evolved in response to the phenology of the host, by allowing females to oviposit in nuts before their full sclerotinization. Adult overwintering appears to be costly because adults postpone reproduction for 1 year, incur a significant weight loss and require feeding before egg laying. Surprisingly, lipids are unaffected during diapause, lipogenesis even being likely in the summer metamorphosis. These results suggest that the lipids involved in egg production may entirely come from the larval stages, whereas the other nutrients are acquired through adult feeding but this remains to be tested. 相似文献
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CHRISTIAN LAFLAMME LOUIS GENDRON NATHALIE TURGEON GENEVIEVE FILION JIM HO CAROLINE DUCHAINE 《Journal of Rapid Methods and Automation in Microbiology》2009,17(1):80-102
Methods for the specific detection of Bacillus spores are needed in many situations such as the recognition of food poisoning. This study presents an experimental design in order to find the best combination of germination conditions leading to a rapid and detectable fluorescent in situ hybridization (FISH) signal from Bacillus cereus spores present in pure cultures and milk samples.
B. cereus ATCC 14579 and HER 1414 were incubated in 20 different growth media by using a combination of various germinants such as sugars, amino acids and dipicolinic acid. Also, three different germination factors were tested: incubation temperature, inoculum concentration and a heat shock treatment. Permeabilization procedure and hybridization time were optimized on the best germination condition found. B. cereus -specific FISH probes were validated under the optimized condition and in detection of spiked B. cereus spores in 1% ultra heat-treated milk samples. FISH-labeled cells were detected by using flow cytometry, and the results were confirmed by fluorescence microscopy. The optimal condition allows the detection of B. cereus spores in less than 2 h. Overall, a ninefold reduction in total time for detection was achieved when comparing with previous works. Therefore, the permeabilization and hybridization optimizations mentioned in this study are major improvements for the detection time of B. cereus spores.
By using the optimized conditions of germination/outgrowth, permeabilization and hybridization, the detection of 103 cfu/mL of Bacillus cereus spores using fluorescent in situ hybridization is possible within 2 h in milk sample. 相似文献
B. cereus ATCC 14579 and HER 1414 were incubated in 20 different growth media by using a combination of various germinants such as sugars, amino acids and dipicolinic acid. Also, three different germination factors were tested: incubation temperature, inoculum concentration and a heat shock treatment. Permeabilization procedure and hybridization time were optimized on the best germination condition found. B. cereus -specific FISH probes were validated under the optimized condition and in detection of spiked B. cereus spores in 1% ultra heat-treated milk samples. FISH-labeled cells were detected by using flow cytometry, and the results were confirmed by fluorescence microscopy. The optimal condition allows the detection of B. cereus spores in less than 2 h. Overall, a ninefold reduction in total time for detection was achieved when comparing with previous works. Therefore, the permeabilization and hybridization optimizations mentioned in this study are major improvements for the detection time of B. cereus spores.
PRACTICAL APPLICATIONS
By using the optimized conditions of germination/outgrowth, permeabilization and hybridization, the detection of 10
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GERALD MOSER CHRISTOPH LEUSCHNER DIETRICH HERTEL SOPHIE GRAEFE NATHALIE SOETHE SUSANNE IOST 《Global Change Biology》2011,17(6):2211-2226
Carbon storage and sequestration in tropical mountain forests and their dependence on elevation and temperature are not well understood. In an altitudinal transect study in the South Ecuadorian Andes, we tested the hypotheses that (i) aboveground net primary production (ANPP) decreases continuously with elevation due to decreasing temperatures, whereas (ii) belowground productivity (BNPP) remains constant or even increases with elevation due to a shift from light to nutrient limitation of tree growth. In five tropical mountain forests between 1050 and 3060 m a.s.l., we investigated all major above‐ and belowground biomass and productivity components, and the stocks of soil organic carbon (SOC). Leaf biomass, stemwood mass and total aboveground biomass (AGB) decreased by 50% to 70%, ANPP by about 70% between 1050 and 3060 m, while stem wood production decreased 20‐fold. Coarse and large root biomass increased slightly, fine root biomass fourfold, while fine root production (minirhizotron study) roughly doubled between 1050 and 3060 m. The total tree biomass (above‐ and belowground) decreased from about 320 to 175 Mg dry mass ha?1, total NPP from ca. 13.0 to 8.2 Mg ha?1 yr?1. The belowground/aboveground ratio of biomass and productivity increased with elevation indicating a shift from light to nutrient limitation of tree growth. We propose that, with increasing elevation, an increasing nitrogen limitation combined with decreasing temperatures causes a large reduction in stand leaf area resulting in a substantial reduction of canopy carbon gain toward the alpine tree line. We conclude that the marked decrease in tree height, AGB and ANPP with elevation in these mountain forests is caused by both a belowground shift of C allocation and a reduction in C source strength, while a temperature‐induced reduction in C sink strength (lowered meristematic activity) seems to be of secondary importance. 相似文献
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VIVIAN DE BUFFRÉNIL NATHALIE BARDET XABIER PEREDA‐SUBERBIOLA BAÂDI BOUYA 《Lethaia: An International Journal of Palaeontology and Stratigraphy》2008,41(1):59-69
The histological organization of the vertebrae of the Maastrichtian squamate Pachyvaranus crassispondylus Arambourg, 1952 , was compared to that of various extant squamates, in order to further document the causes and functional consequences of the so-called 'pachyostosis', frequently observed in Late Cretaceous squamates. The vertebrae of Pachyvaranus are composed of the same basic bone tissue types as those of extant lizards and snakes. In particular, periosteal cortices are made of a pseudolamellar (or 'parallel-fibred') tissue, with radial vascular canals, Sharpey's fibres and conspicuous cyclic growth marks that are strictly identical to that found in extant varanids. Conversely, the vertebrae of Pachyvaranus are extremely compact, whereas those of extant squamates are very cancellous and lightly built. This difference is due to the absence in Pachyvaranus of a broad internal resorption field that, in extant lizards and snakes, transforms compact cortices into loose spongy formations. This absence of inner bone resorption typically corresponds to an osteosclerotic process. In Pachyvaranus , cortical hyperplasy, or pachyostosis stricto sensu , was restricted to the walls of the neural spine. Extreme vertebral porosity is likely to be a primitive condition in squamates, because all lizards and snakes examined in this study display this feature. Therefore, the high vertebral compactness observed in Pachyvaranus would be a derived condition arising from the loss (or de-differentiation) of a morphogenetic process: the broad internal resorption of the vertebrae. Possible palaeoecological bearings of these results are discussed. 相似文献
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