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451.
Sec31 encodes an essential component of the COPII coat required for transport vesicle budding from the endoplasmic reticulum. 总被引:8,自引:3,他引:5
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The COPII vesicle coat protein promotes the formation of endoplasmic reticulum- (ER) derived transport vesicles that carry secretory proteins to the Golgi complex in Saccharomyces cerevisiae. This coat protein consists of Sar1p, the Sec23p protein complex containing Sec23p and Sec24p, and the Sec13p protein complex containing Sec13p and a novel 150-kDa protein, p150. Here, we report the cloning and characterization of the p150 gene. p150 is encoded by an essential gene. Depletion of this protein in vivo blocks the exit of secretory proteins from the ER and causes an elaboration of ER membranes, indicating that p150 is encoded by a SEC gene. Additionally, overproduction of the p150 gene product compromises the growth of two ER to Golgi sec mutants: sec16-2 and sec23-1. p150 is encoded by SEC31, a gene isolated in a genetic screen for mutations that accumulate unprocessed forms of the secretory protein alpha-factor. The sec31-1 mutation was mapped by gap repair, and sequence analysis revealed an alanine to valine change at position 1239, near the carboxyl terminus. Sec31p is a phosphoprotein and treatment of the Sec31p-containing fraction with alkaline phosphatase results in a 50-75% inhibition of transport vesicle formation activity in an ER membrane budding assay. 相似文献
452.
Molecular phylogenetic evidence that the phylum Haplosporidia has an alveolate ancestry 总被引:1,自引:0,他引:1
The phylogenetic position of the phylum Haplosporidia among other protists
was investigated with the complete 16S-like rRNA gene sequences from two
species in the phylum: Haplosporidium nelsoni, a parasite of oysters, and
Minchinia teredinis, a parasite of shipworms. Because the lack of obvious
morphological homologies with other protists hampered decisions regarding
taxonomic composition for sequence alignment and phylogenetic analysis, the
complete sequences for these two haplosporidians were directed as search
queries to the blast/ncbi.nlm.nih.gov electronic mail server. The results
of this heuristic similarity search provided a basis for constructing a
preliminary higher-taxonomic-level analysis comparing the haplosporidians
with species from the slime molds, fungi, algae, amoebae, ciliates,
dinoflagellates, and apicomplexans. Maximum parsimony yielded equivocal
results, whereas transversionally weighted parsimony suggested an affinity
with the alveolates (i.e., the ciliates, dinoflagellates, and
apicomplexans). Multiple alignment of the two haplosporidian sequences
against 17 taxa in a secondary analysis focusing on the alveolates and
subsequent parsimony analysis placed the phylum Haplosporidia as a
monophyletic group within the Alveolata and as a taxon of equal rank with
the other three alveolate phyla. The precise placement within the Alveolata
was sensitive to weighting.
相似文献
453.
Summary This study is mainly concerned with some parameters contributing to growth as indicators of difference in drought resistance
and salt tolerance of wheat and barley cultivars. Parameters made use of are: transpiration efficiency (‘dry matter/transpiration’
ratio), ‘leaf/root’ ratio, chlorophyll content, chlorophyll stability to heat and anatomical modifications. The results revealed
that transpiration efficiency is much higher in mexican ‘super-x’ wheat than in the egyptian ‘Giza-155’ cultivar under reduced
soil water matric- or osmotic potentials. Chlorophyll content increased in super-x with decreasing soil water potential while
chlorophyll heat stability decreased. The reverse is true in Giza-155 cultivar. Decreased leaf/root ratio in super-x is interpretted
in favour of more beneficial water balance in this cultivar. Development of more sclerenchyma in its stems supports this judgement.
Of barley cultivars tested, Borg El-Arab is favoured for drought resistance and Giza-117 for salt tolerance. re]19751014 相似文献
454.
陕西省主要蔬菜产区蔬菜重金属污染状况分析与评价 总被引:7,自引:0,他引:7
以GB18406.1-2001无公害蔬菜标准为评价依据.对陕西省8个蔬菜主产区的127个蔬菜样品中汞、砷、铅、镉和铬5种重金属污染状况进行分析评价。结果表明,陕西省主要蔬菜产区蔬菜重金属污染以铅污染为主。茄果类和瓜菜类蔬菜铅污染严重,绿叶类蔬菜铅污染相对较轻.其它4种重金属污染未超过国家标准,其中各产区均未检出镉。铅污染程度的大小顺序足成阳秦都〉宝鸡岐山蔡家坡〉汉中汉台〉渭南临渭。成阳泾阳、宝鸡太白、汉中城固、渭南大荔蔬菜产区5种蓖金瞒均未超标.具备发展无公害蔬菜生产基地条件。 相似文献
455.
目的研究膀胱移行细胞癌(TCC)中黑色素瘤抗原(MAGE)基因表达。方法逆转录聚合酶链反应(RT-PCR)技术检测20例膀胱TCC患者癌组织和3株膀胱TCC细胞株T24、EJ、BIU87中MAGE-A1、A2、A3、A4基因mRNA表达。结果20例膀胱TCC癌组织中19例(95%)至少表达一种MAGE-A基因,12例MAGE-A1阳性(60%),16例MAGE-A2阳性(80%),11例MAGE-A3阳性(55%),18例MAGE-A4阳性(90%),MAGE-A1-4均阳性8例(40%)。膀胱TCC细胞株T24中MAGE-A1-4基因均表达,EJ中MAGE-A3、A4基因表达,BIU87中MAGE-A2、A3、A4基因表达。结论MAGE基因在膀胱TCC中有较高表达,可望成为膀胱TCC免疫治疗的靶基因。 相似文献
456.
菠萝蜜中香气成分分析 总被引:8,自引:0,他引:8
纳智 《热带亚热带植物学报》2004,12(6):538-540
用溶剂法提取菠萝蜜中的香气成分,用GC-MS联用技术鉴定了82个化合物,占香气成分总量的94.66%,主要为脂肪酸类以及酯类、醇类、烷氧基烷烃类和酮类物质,主要成分为亚油酸(24.10%),棕榈酸(15.72%)和油酸(6.16%)。 相似文献
457.
CD28-independent costimulation of T cells in alloimmune responses. 总被引:15,自引:0,他引:15
A Yamada K Kishimoto V M Dong M Sho A D Salama N G Anosova G Benichou D A Mandelbrot A H Sharpe L A Turka H Auchincloss M H Sayegh 《Journal of immunology (Baltimore, Md. : 1950)》2001,167(1):140-146
T cell costimulation by B7 molecules plays an important role in the regulation of alloimmune responses. Although both B7-1 and B7-2 bind CD28 and CTLA-4 on T cells, the role of B7-1 and B7-2 signaling through CTLA-4 in regulating alloimmune responses is incompletely understood. To address this question, we transplanted CD28-deficient mice with fully allogeneic vascularized cardiac allografts and studied the effect of selective blockade of B7-1 or B7-2. These mice reject their grafts by a mechanism that involves both CD4(+) and CD8(+) T cells. Blockade of CTLA-4 or B7-1 significantly accelerated graft rejection. In contrast, B7-2 blockade significantly prolonged allograft survival and, unexpectedly, reversed the acceleration of graft rejection caused by CTLA-4 blockade. Furthermore, B7-2 blockade prolonged graft survival in recipients that were both CD28 and CTLA-4 deficient. Our data indicate that B7-1 is the dominant ligand for CTLA-4-mediated down-regulation of alloimmune responses in vivo and suggest that B7-2 has an additional receptor other than CD28 and CTLA-4 to provide a positive costimulatory signal for T cells. 相似文献
458.
气候变化对内蒙古中东部沙地-湿地镶嵌景观的影响 总被引:3,自引:0,他引:3
利用小波分析和遥感技术相结合的方法,研究了气候变化对1961—2005年内蒙古中东部沙地-湿地镶嵌景观的影响.结果表明:1961—2005年,研究区年均气温呈直线上升趋势,增温率为0.32 ℃·(10 a)-1,年降水量变化呈现30 a的长周期和15 a的短周期,年均风速呈下降趋势,10 a的风速减小率为0.26 m·s-1;研究区东南部存在一个气候变化恒定区域,由此恒定区向西北进入浑善达克沙地腹地,向东逐步进入科尔沁沙地腹地;2010年以前,研究区仍将维持气温升高、降水偏少、风速减小的趋势;受气候暖干化的影响,浑善达克沙地及其周围湿地的总面积呈逐年缩小趋势,随着沙地内部植被的退化,流动沙地不断扩大、湿地萎缩、湖泊干涸,而相应的固定和半固定沙地面积则明显减少. 相似文献
459.
460.
14种菊科植物提取物对菜青虫的杀虫活性 总被引:28,自引:1,他引:27
用胜红蓟、黄花蒿、青蒿、艾蒿、鬼针草、三叶鬼针草、鱼眼草、小鱼眼草、紫茎泽兰、千里光、希签草、万寿菊、肿柄菊、苍耳等14种菊科植物的石油醚、氯仿、丙酮、乙醇、甲醇和水提取物对菜青虫5龄幼虫的生测结果表明,黄花蒿、青蒿、艾蒿、鱼眼草、小鱼眼草、紫茎泽兰、万寿菊等7种菊科植物甲醇和乙醇提取物具有较高的杀虫活性。此结果为进一步从菊科植物中提取,分离和筛选高活性杀虫活性成分奠定了基础。 相似文献