The Characteristics of Primary Hybrids Obtained in Crosses between Common Wheat from China and Cultivated Rye

Russian Journal of Genetics - The gene pool of winter common wheat from China maintained in the VIR collection is unique in the variety of alleles of the genes of selection-valuable traits and...     

Establishing a Cultivable Cell Line of the Tick Dermacentor marginatus

Russian Journal of Genetics - Owing to the changes in the general ecological situation in Russia, livestock losses from ovine anaplasmosis have rapidly increased in recent years. The development of...     

Mitochondrial Genomes of Flor Yeast Strains Are Characterized by Low Genetic Variability

Russian Journal of Genetics - High concentrations of ethanol and oxidative stress can cause a mutagenic effect on mitochondrial genomes (mtDNAs) of flor yeasts. We performed a comparative analysis...     

Using neutron crystallography to elucidate the basis of selective inhibition of carbonic anhydrase by saccharin and a derivative

Up-regulation of carbonic anhydrase IX (CA IX) expression is an indicator of metastasis and associated with poor cancer patient prognosis. CA IX has emerged as a cancer drug target but development of isoform-specific inhibitors is challenging due to other highly conserved CA isoforms. In this study, a CA IX_mimic construct was used (CA II with seven point mutations introduced, to mimic CA IX active site) while maintaining CA II solubility that make it amenable to crystallography. The structures of CA IX_mimic unbound and in complex with saccharin (SAC) and a saccharin-glucose conjugate (SGC) were determined using joint X-ray and neutron protein crystallography. Previously, SAC and SGC have been shown to display CA isoform inhibitor selectivity in assays and X-ray crystal structures failed to reveal the basis of this selectivity. Joint X-ray and neutron crystallographic studies have shown active site residues, solvent, and H-bonding re-organization upon SAC and SGC binding. These observations highlighted the importance of residues 67 (Asn in CA II, Gln in CA IX) and 130 (Asp in CA II, Arg in CA IX) in selective CA inhibitor targeting.     

Tumor Specific Peptides Selected for Targeted Delivery of Therapeutic Agents to Glioma Human Cells

Russian Journal of Bioorganic Chemistry - Brain tumors are among the most intractable types of malignant neoplasms. Despite advances in the treatment of cancer, in particular, the development of...     

Initiated Oxidation of Phosphatidylcholine Liposomes with Some Functional Nutraceuticals

Russian Journal of Bioorganic Chemistry - Design and research of liposome structures on the basis of soy phosphatidylcholine (PC) as nanocontainers for delivery of various functional nutraceuticals...     

Features of Metabolomic Profiles in Different Stages of Ontogenesis in Prunella vulgaris (Lamiaceae) Grown in a Climate Chamber

Russian Journal of Bioorganic Chemistry - Metabolic analysis of methanol extracts of leaves of Prunella vulgaris L. (Lamiaceae) grown in a climate chamber has been carried out using gas...     

Structure of Chromophores in GFP-Like Proteins: X-Ray Data

Russian Journal of Bioorganic Chemistry - The discovery of fluorescent proteins (FP) in 1962 and the following design of genetically encoded biomarkers and biosensors revolutionized the study of...     

High-Speed Recording of Vacuum Arc Discharge Images in the Optical Range

Plasma Physics Reports - Switching of a short vacuum gap with an auxiliary discharge over the surface of a dielectric has been studied via high-speed recording of images of discharge plasma...