Limited proteolysis of human erythrocyte Ca2+-ATPase in membrane-bound form. Identification of calmodulin-binding fragments

Water-soluble and membrane-bound calmodulin-binding polypeptides formed upon limited proteolysis of erythrocyte ghosts were isolated by means of affinity chromatography. Immune blotting revealed that all isolated fragments originated from Ca2+-ATPase. Among the fragments obtained those having formed an acylphosphate intermediate were identified. The N-terminal residue of purified intact Ca2+-ATPase was shown to be blocked (probably acylated).     

Reactivity and binding of benzo(a)pyrene diol epoxide to poly(dG-dC).(dG-dC) and poly(dG-m5dC).(dG-m5dC) in the B and Z forms

The physical and covalent binding of the carcinogen benzo(a)pyrene-7,8-diol-9,10-oxide (BaPDE) to poly(dG-dC).(dG-dC) and poly(dG-m5dC).(dG-m5dC) in the B and Z forms were studied utilizing absorbance, fluorescence and linear dichroism techniques. In the case of poly(dG-dC).(dG-dC) the decrease in the covalent binding of BaPDE with increasing NaCl concentration (0.1-4 M) as the B form is transformed to the Z form is attributed to the effects of high ionic strengths on the reactivity and physical binding of BaPDE to the polynucleotides; these effects tend to obscure differences in reactivities with the B and Z forms of the nucleic acids. In the case of poly(dG-m5dC).(dG-m5dC) the B-to-Z transition is induced at low ionic strength (2 mM NaCl + 10 microM Co(NH3)6Cl3) and the covalent binding is found to be 2-3-times lower to the Z form than to the B form. Physical binding of BaPDE by intercalation, which precedes the covalent binding reaction, is significantly lower in the Z form than in the B form, thus accounting, in part, for the lower covalent binding. The linear dichroism characteristics of BaPDE covalently bound to the Z and B forms of poly(dG-m5dC).(dG-m5dC) are consistent with nonintercalative, probably external conformations of the aromatic pyrenyl residues.     

Seasonal distribution of enteroviruses and adenoviruses in domestic sewage

A seasonal distribution of enteroviruses and adenoviruses in raw sewage effluents of Athens, Greece, was observed over a 15-month surveillance period. All 36 samples tested were positive for both virus groups. Adenovirus concentration levels ranged from 70 to 3200 cytopathic units per litre of sample, whereas the corresponding values for enteroviruses were 90-900 cytopathic units per litre. Peak values for adenoviruses were recorded during the months of April and June 1983, whereas for enteroviruses the peak was recorded in September 1983. All three types of poliovirus were present. Coxsackievirus types B-1, B-2, B-4, and B-5 and echovirus type 7 were also isolated. Adenovirus types 1, 2, 3, 5, 7, and 15 were detected as well.     

Furosemide-sensitive Na+-K+ cotransport and cellular metabolism in human erythrocytes

Metabolic depletion of human red cells with 2-deoxy-D-glucose in the presence of EGTA decreased ATP to about 4% of the initial value and increased total ouabain- and furosemide-resistant Na+ and K+ effluxes by 20% and 100%, respectively, and furosemide-sensitive Na+ and K+ effluxes by 100% and 60%, respectively. When ATP was restored, all the components of Na+ and K+ fluxes measured returned to baseline levels suggesting a metabolic dependence.     

Regulation of the Oxidative Processes in the Organs of Mice under the Effects of Chemical and Physical Factors at Low Doses

Biophysics - The effects of the separate and combined action of lead nitrate in a wide range of doses, uranyl nitrate, and chronic low-dose gamma irradiation on the formation of oxidative stress in...     

Restoring avian wing digits

The precise identification of the digits of the avian wing is of importance in evolutionary studies. If the digits are numbered two, three and four, this has been taken to suggest that birds are not descended directly from dinosaurs. If the digits are numbered one, two and three, dinosaur origins become more plausible. Studies of the development of the avian wing have failed to resolve this dilemma. However, in some instances, it is possible to deduce information about evolutionary morphologies by manipulating development experimentally. We grafted beads loaded with fibroblast growth factor 4 into the distal tip of chick wing buds at times when the apical ectodermal ridge is regressing. The consequence was that the cartilage structure conventionally labelled ''element 5'' increased dramatically in size and acquired a digit-like morphology in some instances. Corresponding changes in soft tissue morphology were also observed. We conclude that it may be possible to resolve the issue of avian digit homology by the induction of experimental atavisms of this kind.     

Cruoriella rhodoliths from shallow-water back reef environments in La Parguera, Puerto Rico (Caribbean Sea)

The occurrence of shallow-water (0.9 to 1.3 m) rhodoliths in back reef environments in southwest Puerto Rico is reported. The rhodoliths were generally cylindrical, discoidal or irregular in shape with an average longest dimension of 7.2 cm. They occurred at a maximum density of 524 m⁻². The rhodoliths were composed of mostly coral nuclei with concentric laminations of aragonite-producing Cruoriella armorica (Peyssonneliaceae, Rhodophyta). Maximum Cruoriella accretion around coral nuclei was 30 mm although accretions of 1 to 20 mm were more common. Based on measurements of Cruoriella accretion, these shallow water rhodoliths are estimated to have minimum ages of 12 to 24 years. It is further estimated that approximately 2% of the rhodoliths are turned over daily. Accepted: 1 October 1999     

Human Discrimination of the Angular Velocity of the Vertical Movement of an Acoustic Image in Opposite Directions

The discrimination of the angular velocity of ventrodorsal and dorsoventral movement of an acoustic image was studied in nine test subjects. The experiments were performed using an apparent movement produced by consecutive activation of loudspeakers located along an arc in the vertical plane. The differential thresholds were measured by the minimum increment method. As the velocity of an acoustic image movement in opposite directions increased, the values of its mean absolute differential thresholds increased monotonically. Regression lines plotted by linear approximation of these values did not differ significantly.