全文获取类型
收费全文 | 1122篇 |
免费 | 54篇 |
出版年
2024年 | 2篇 |
2023年 | 4篇 |
2022年 | 9篇 |
2021年 | 30篇 |
2020年 | 18篇 |
2019年 | 9篇 |
2018年 | 16篇 |
2017年 | 17篇 |
2016年 | 30篇 |
2015年 | 60篇 |
2014年 | 66篇 |
2013年 | 86篇 |
2012年 | 89篇 |
2011年 | 84篇 |
2010年 | 49篇 |
2009年 | 54篇 |
2008年 | 56篇 |
2007年 | 72篇 |
2006年 | 51篇 |
2005年 | 56篇 |
2004年 | 48篇 |
2003年 | 58篇 |
2002年 | 43篇 |
2001年 | 33篇 |
2000年 | 31篇 |
1999年 | 29篇 |
1998年 | 8篇 |
1997年 | 2篇 |
1996年 | 4篇 |
1995年 | 1篇 |
1994年 | 5篇 |
1993年 | 4篇 |
1992年 | 5篇 |
1991年 | 6篇 |
1990年 | 8篇 |
1989年 | 13篇 |
1988年 | 1篇 |
1987年 | 5篇 |
1985年 | 1篇 |
1984年 | 1篇 |
1980年 | 1篇 |
1979年 | 3篇 |
1977年 | 2篇 |
1975年 | 2篇 |
1974年 | 1篇 |
1970年 | 1篇 |
1914年 | 1篇 |
1891年 | 1篇 |
排序方式: 共有1176条查询结果,搜索用时 15 毫秒
151.
Seongyup Kim Pil Young Jung Jin Suk Lee Soonjae Hwang Joon Hyung Sohn Yongdae Yoon Keum Seok Bae Young Woo Eom 《Cell biology international》2021,45(12):2443-2451
Skeletal muscle satellite cells (SkMSCs) play crucial roles in muscle fiber maintenance, repair, and remodeling; however, it remains unknown if these properties are preserved in cultured SkMSCs. In this study, we investigated the characteristics of cultured SkMSCs and their ability to regulate the activity of M1 macrophages. SkMSCs grew well with an average population doubling time of 26.26 ± 6.85 h during 10 passages (P). At P5, Pax7, MyoD, cluster of differentiation (CD)34, and CD56 were not expressed in SkMSCs, but the MSC markers CD73, CD105, and CD90 were expressed and the cells were differentiated into adipocytes and osteoblasts. When SkMSCs were cocultured with macrophages, interleukin (IL)-1β secretion was decreased, prostaglandin (PG)E2 was produced in coculture, and cyclooxygenase-2 protein was induced in an SkMSC-dependent manner. Hepatocyte growth factor (HGF) was highly secreted by monocultured SkMSCs; interferon-γ and lipopolysaccharide reduced its expression level. However, HGF expression recovered when SkMSCs and macrophages were cocultured. Although exogenous PGE2 upregulated macrophage pro-IL-1β expression, it suppressed the secretion of cleaved IL-1β. In contrast, HGF decreased active IL-1β secretion without affecting pro-IL-1β expression. Co-treatment of macrophages with HGF and PGE2 reduced pro-IL-1β expression level and active IL-1β secretion. Our results suggest that SkMSCs lose their satellite cell properties during serial passaging but acquire mesenchymal stem cell properties including the ability to exert an anti-inflammatory response for macrophages through PGE2 and HGF. 相似文献
152.
153.
Jung-Min Shin Mi Yoon Kim Kyung-Cheol Sohn So-Young Jung Hae-Eul Lee Jae Woo Lim Sooil Kim Young-Ho Lee Myung Im Young-Joon Seo Chang Deok Kim Jeung-Hoon Lee Young Lee Tae-Jin Yoon 《PloS one》2014,9(4)
Nrf2 plays a role in protection of cells against oxidative stress and xenobiotic damage by regulating cytoprotective genes. In this study, we investigated the effect of Nrf2 on melanogenesis in normal human melanocytes (NHMCs). When NHMCs were transduced with a recombinant adenovirus expressing Nrf2, melanin synthesis was significantly decreased. Consistent with this result, overexpression of Nrf2 decreased the expression of tyrosinase and tyrosinase-related protein 1. The inhibitory effect of Nrf2 was reversed by overexpression of Keap1, an intracellular regulator of Nrf2. Interestingly, Nrf2 overexpression resulted in marked activation of PI3K/Akt signaling. Conversely, inhibition of PI3K activity by treatment with wortmannin reversed the depigmentary effects of Nrf2. Taken together, these results strongly suggest that Nrf2 negatively regulates melanogenesis by modulating the PI3K/Akt signaling pathway. 相似文献
154.
155.
Charge Transport Modulation of a Flexible Quantum Dot Solar Cell Using a Piezoelectric Effect
下载免费PDF全文
![点击此处可从《Liver Transplantation》网站下载免费的PDF全文](/ch/ext_images/free.gif)
Yuljae Cho Paul Giraud Bo Hou Young‐Woo Lee John Hong Sanghyo Lee Sangyeon Pak Juwon Lee Jae Eun Jang Stephen M. Morris Jung Inn Sohn SeungNam Cha Jong Min Kim 《Liver Transplantation》2018,8(3)
Colloidal quantum dots are promising materials for flexible solar cells, as they have a large absorption coefficient at visible and infrared wavelengths, a band gap that can be tuned across the solar spectrum, and compatibility with solution processing. However, the performance of flexible solar cells can be degraded by the loss of charge carriers due to recombination pathways that exist at a junction interface as well as the strained interface of the semiconducting layers. The modulation of the charge carrier transport by the piezoelectric effect is an effective way of resolving and improving the inherent material and structural defects. By inserting a porous piezoelectric poly(vinylidenefluoride‐trifluoroethylene) layer so as to generate a converging electric field, it is possible to modulate the junction properties and consequently enhance the charge carrier behavior at the junction. This study shows that due to a reduction in the recombination and an improvement in the carrier extraction, a 38% increase in the current density along with a concomitant increase of 37% in the power conversion efficiency of flexible quantum dots solar cells can be achieved by modulating the junction properties using the piezoelectric effect. 相似文献
156.
Jaehoon Jung Kwondo Kim DongAhn Yoo Chanho Lee Jungsun Kang Kyungjin Cho Dae-Kyung Kang Woori Kwak Sook Hee Yoon Hawsun Sohn Heebal Kim Seoae Cho 《Genes & genomics.》2018,40(2):217-223
Lactobacillus plantarum is one of the widely-used probiotics and there have been a large number of advanced researches on the effectiveness of this species. However, the difference between previously reported plantarum strains, and the source of genomic variation among the strains were not clearly specified. In order to understand further on the molecular basis of L. plantarum on Korean traditional fermentation, we isolated the L. plantarum GB-LP4 from Korean fermented vegetable and conducted whole genome assembly. With comparative genomics approach, we identified the candidate genes that are expected to have undergone evolutionary acceleration. These genes have been reported to associate with the maintaining homeostasis, which are generally known to overcome instability in external environment including low pH or high osmotic pressure. Here, our results provide an evolutionary relationship between L. plantarum species and elucidate the candidate genes that play a pivotal role in evolutionary acceleration of GB-LP4 in high osmolarity environment. This study may provide guidance for further studies on L. plantarum. 相似文献
157.
Plasma T4 (L-thyroxine) concentrations in the viviparous rockfish Sebastes inermis showed a peak (119·3±27·8 ng ml−1 ) during the development of embryos. Ovarian T4 concentrations increased during vitellogenesis and final maturation and decreased during embryogenesis.However, total T4 content within the ovary increased continuously up to the larval stages. Plasma oestradiol-17β, (E2 ) concentrations peaked at the final maturation stage and then recovered to the level seen during the non-reproductive periods. Plasma testosterone (T) concentrations showed a peak (5·33±1·08 ng ml−1 ) at the embryo stage and high levels were maintained throughout the gestation period. Plasma T4 concentrations during the embryo stage and ovarian T4 content during vitellogenesis were much higher than the levels reported in oviparous fishes. These data suggest that in viviparous rockfish, T4 may be required for sustaining gestation or embryo development within the maternal body. In addition, the high content of total T4 in the ovary implies that there probably does exist a maternal-embryo relationship during gestation as well as during vitellogenesis. 相似文献
158.
Angela Gullard Jelica Gluhak-Heinrich Silvana Papagerakis Philip Sohn Aaron Unterbrink Shuo Chen Mary MacDougall 《The journal of histochemistry and cytochemistry》2016,64(4):224-236
Matrix extracellular phosphoglycoprotein (MEPE) is an extracellular matrix protein found in dental and skeletal tissues. Although information regarding the role of MEPE in bone and disorders of phosphate metabolism is emerging, the role of MEPE in dental tissues remains unclear. We performed RNA in situ hybridization and immunohistochemistry analyses to delineate the expression pattern of MEPE during embryonic and postnatal development in craniofacial mineralizing tissues. Mepe RNA expression was seen within teeth from cap through root formation in association with odontoblasts and cellular cementoblasts. More intense expression was seen in the alveolar bone within the osteoblasts and osteocytes. MEPE immunohistochemistry showed biphasic dentin staining in incisors and more intense staining in alveolar bone matrix and in forming cartilage. Analysis of Mepe null mouse molars showed overall mineralized tooth volume and density of enamel and dentin comparable with that of wild-type samples. However, Mepe-/- molars exhibited increased thickness of predentin, dentin, and enamel over controls and decreased gene expression of Enam, Bsp, Dmp1, Dspp, and Opn by RT-PCR. In vitro Mepe overexpression in odontoblasts led to significant reductions in Dspp reporter activity. These data suggest MEPE may be instrumental in craniofacial and dental matrix maturation, potentially functioning in the maintenance of non-mineralized matrix. 相似文献
159.
Hyeong-Kyu Jeon Hansol Park Dongmin Lee Seongjun Choe Kyu-Heon Kim Woon-Mok Sohn Keeseon S. Eom 《The Korean journal of parasitology》2016,54(2):181-185
Human sparganosis is a zoonotic disease caused by infection with larval forms (procercoid/plerocercoid) of Spirometra spp. The purpose of this study was to identify Spirometra spp. of infected snakes using a multiplex PCR assay and phylogenetic analysis of mitochondrial DNA sequence data from the spargana of terrestrial snakes obtained from Korea and China. A total of 283 snakes were obtained that included 4 species of Colubridae comprising Rhabdophis tigrinus tigrinus (n=150), Dinodon rufozonatum rufozonatum (n=64), Elaphe davidi (n=2), and Elaphe schrenkii (n=7), and 1 species of Viperidae, Agkistrodon saxatilis (n=60). The snakes were collected from the provinces of Chungbuk, Chungnam, and Gyeongbuk in Korea (n=161), and from China (n=122). The overall infection rate with spargana was 83% (235/283). The highest was recorded for D. rufozonatum rufozonatum (100%), followed by A. saxatilis (85%) and R. tigrinus tigrinus (80%), with a negative result for E. davidi (0%) and E. schrenkii (0%). The sequence identities between the spargana from snakes (n=50) and Spirometra erinaceieuropaei () or S. decipiens ( KJ599680) control specimens were 90.8% and 99.2%, respectively. Pairwise genetic distances between spargana (n=50) and S. decipiens ranged from 0.0080 to 0.0107, while those between spargana and S. erinaceieuropaei ranged from 0.1070 to 0.1096. In this study, all of the 904 spargana analyzed were identified as S. decipiens either by a multiplex PCR assay (n=854) or mitochondrial cox1 sequence analysis (n=50). KJ599679相似文献
160.