排序方式: 共有38条查询结果,搜索用时 15 毫秒
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Rogers E Petreas M Park JS Zhao G Charles MJ 《Journal of chromatography. B, Analytical technologies in the biomedical and life sciences》2004,813(1-2):269-285
The separation of organochlorine pesticides (OCPs), polychlorinated biphenyls (PCBs), and polybrominated diphenyl ether (PBDE) congeners was evaluated on four capillary columns: 60 m x 0.25 mm i.d., 0.25 microm film thickness RTX-5MS and DB-XLB capillary columns, and 60 m x 0.18 mm i.d., 0.25 microm film thickness DB-XLB and DB-5MS capillary columns. Based on performance, capacity, and cost, the RTX-5MS (60 m x 0.25 mm i.d., 0.25 microm thickness) and the DB-XLB (60 m x 0.25 mm i.d., 0.25 microm film thickness) were selected for the analysis of human serum extracts by using gas chromatography/electron-capture detection. In contrast to previous studies, the oven temperature program affords the separation of congeners that are not separated by using other combinations of capillary columns, most notably PBDE-47 and PCB 180. In addition, the method enables determination of OCPs, PCBs, and PBDEs prevalent in a single extract of serum, which can lead to considerable time savings in the analysis of large number of samples collected for epidemiologic studies. 相似文献
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The family of 14‐3‐3 proteins and specifically 14‐3‐3σ are up‐regulated during the development of renal pathologies
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Myrto Rizou Eleni A. Frangou Filio Marineli Niki Prakoura Jerome Zoidakis Harikleia Gakiopoulou George Liapis Panagiotis Kavvadas Christos Chatziantoniou Manousos Makridakis Antonia Vlahou John Boletis Demetrios Vlahakos Dimitrios Goumenos Evgenios Daphnis Christos Iatrou Aristidis S. Charonis 《Journal of cellular and molecular medicine》2018,22(9):4139-4149
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Ferdinand Greiss Myrto Deligiannaki Christophe Jung Ulrike Gaul Dieter Braun 《Biophysical journal》2016,110(4):939-946
In multicellular organisms, single-fluorophore imaging is obstructed by high background. To achieve a signal/noise ratio conducive to single-molecule imaging, we adapted reflected light-sheet microscopy (RLSM) to image highly opaque late-stage Drosophila embryos. Alignment steps were modified by means of commercially available microprisms attached to standard coverslips. We imaged a member of the septate-junction complex that was used to outline the three-dimensional epidermal structures of Drosophila embryos. Furthermore, we show freely diffusing single 10 kDa Dextran molecules conjugated to one to two Alexa647 dyes inside living embryos. We demonstrate that Dextran diffuses quickly (∼6.4 μm2/s) in free space and obeys directional movement within the epidermal tissue (∼0.1 μm2/s). Our single-particle-tracking results are supplemented by imaging the endosomal marker Rab5-GFP and by earlier reports on the spreading of morphogens and vesicles in multicellular organisms. The single-molecule results suggest that RLSM will be helpful in studying single molecules or complexes in multicellular organisms. 相似文献
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Myrto Dimakopoulou Sotirios E. Tjamos Polymnia P. Antoniou Amedeo Pietri Paola Battilani Nikolaos Avramidis Emmanouil A. Markakis Eleftherios C. Tjamos 《Biological Control》2008,46(2):158-165
Phyllosphere yeasts were isolated from leaves of vine (Vitis vinifera L.) canes and evaluated in a detached berry assay for their ability to suppress Aspergillus carbonarius (Order: Eurotiales, Family: Trichocomaceae) growth. Seventeen of the 21 yeast isolates significantly reduced A. carbonarius growth, i.e. sour rot infection compared to untreated controls in laboratory tests. The most effective yeast isolate Aureobasidium pullulans (Order: Dothideales, Family: Dothioraceae), isolate Y-1, was field tested on two varieties of red grape, Grenache Rouge and Agiorgitiko located on the Island of Rhodes and in Corinthos County, Greece. It was demonstrated that A. pullulans Y-1 was as effective as the commercial fungicide fludioxonil + cyprodinil, in reducing sour rot infection, A. carbonarius presence on berries at harvest and ochratoxin A contamination in must. 相似文献
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Apostolis Papapostolou Kostas Spengos Irene Fylaktou Myrto Poulou Ilias Gountas Sophia Kitsiou-Tzeli Emmanuel Kanavakis Maria Tzetis 《Gene》2014
ALOX5AP (5-lipoxygenase) has been recognized as a susceptibility gene for stroke. Using a case–control design, the whole coding and adjoining intronic regions of ALOX5AP were sequenced to study the role of SNPs and their interplay with other risk factors in Greek patients with stroke. Patients (n = 213) were classified by the Trial of Org 10172 in Acute Stroke Treatment (TOAST). Their mean age of was 58.9 ± 14.64, comprising 145 males. The control group consisted of 210 subjects, ethnicity, sex and age matched, with no stroke history. Risk factors (hyperlipidemia, hypertension, atrial fibrillation, migraine, CAD, diabetes, smoking and alcohol consumption) were assessed as confounding factors and comparisons were done using logistic regression analysis. SNPs rs4769055, rs202068154 and rs3803277 located in intronic regions of the gene and according to in silico programs EX_SKIP and HSF possibly affecting splicing of exons 1 and 2 of ALOX5AP, showed significantly different frequencies between patients and controls. The genotype frequencies of rs4769055: AA, of rs202068154: AC and of rs3803277: CA were significantly higher (p < 0.001, 0.058) in controls than in patients. The results were indicative of a protective role of the three SNPs either in homozygosity or heterozygosity for MAF and more specifically rs3803277: CA/AA genotypes were protective against SVO stroke subtype. 相似文献