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排序方式: 共有214条查询结果,搜索用时 15 毫秒
31.
Hanan Sela Smadar Ezrati Pnina Ben-Yehuda Jacob Manisterski Eduard Akhunov Jan Dvorak Adina Breiman Abraham Korol 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2014,127(11):2453-2463
Key Message
Rapid LD decay in wild emmer population from Israel allows high-resolution association mapping. Known and putative new stripe rust resistance genes were found.Abstract
Genome-wide association mapping (GWAM) is becoming an important tool for the discovery and mapping of loci underlying trait variation in crops, but in the wild relatives of crops the use of GWAM has been limited. Critical factors for the use of GWAM are the levels of linkage disequilibrium (LD) and genetic diversity in mapped populations, particularly in those of self-pollinating species. Here, we report LD estimation in a population of 128 accessions of self-pollinating wild emmer, Triticum turgidum ssp. dicoccoides, the progenitor of cultivated wheat, collected in Israel. LD decayed fast along wild emmer chromosomes and reached the background level within 1 cM. We employed GWAM for the discovery and mapping of genes for resistance to three isolates of Puccinia striiformis, the causative agent of wheat stripe rust. The wild emmer population was genotyped with the wheat iSelect assay including 8643 gene-associated SNP markers (wheat 9K Infinium) of which 2,278 were polymorphic. The significance of association between stripe rust resistance and each of the polymorphic SNP was tested using mixed linear model implemented in EMMA software. The model produced satisfactory results and uncovered four significant associations on chromosome arms 1BS, 1BL and 3AL. The locus on 1BS was located in a region known to contain stripe rust resistance genes. These results show that GWAM is an effective strategy for gene discovery and mapping in wild emmer that will accelerate the utilization of this genetic resource in wheat breeding. 相似文献32.
Kumru OS Schulze RJ Rodnin MV Ladokhin AS Zückert WR 《Journal of bacteriology》2011,193(11):2814-2825
The dimeric OspC/Vsp family surface lipoproteins of Borrelia spirochetes are crucial to the transmission and persistence of Lyme borreliosis and tick-borne relapsing fever. However, the requirements for their proper surface display remained undefined. In previous studies, we showed that localization of Borrelia burgdorferi monomeric surface lipoprotein OspA was dependent on residues in the N-terminal "tether" peptide. Here, site-directed mutagenesis of the B. burgdorferi OspC tether revealed two distinct regions affecting either release from the inner membrane or translocation through the outer membrane. Determinants of both of these steps appear consolidated within a single region of the Borrelia turicatae Vsp1 tether. Periplasmic OspC mutants still were able to form dimers. Their localization defect could be rescued by the addition of an apparently structure-destabilizing C-terminal epitope tag but not by coexpression with wild-type OspC. Furthermore, disruption of intermolecular Vsp1 salt bridges blocked dimerization but not surface localization of the resulting Vsp1 monomers. Together, these results suggest that Borrelia OspC/Vsp1 surface lipoproteins traverse the periplasm and the outer membrane as unfolded monomeric intermediates and assemble into their functional multimeric folds only upon reaching the spirochetal surface. 相似文献
33.
G. A. Savchenko T. M. Volkova A. A. Vasilevskii Yu. V. Korol’kova E. V. Grishin Ya. A. Boichouk O. A. Krishtal 《Neurophysiology》2011,42(6):387-391
We examined effects of purotoxin 1 (PT1), a component of the venom of Geolycosa spiders, on a few voltageand ligand-operated ion channels present in the plasma membrane of sensory neurons from the rat
dorsal root ganglia (DRGs). Purotoxin 1 in a 100 nM concentration evoked no changes in ion currents through voltage-operated
sodium, potassium, and calcium channels in the membranes of isolated sensory neurons. This agent was also found to be ineffective
with respect to capsaicin-sensitive receptor-channel complexes (TRPV1). Testing of the effects of PT1 on purinergic receptor-channel
complexes P2X3, P2X2, and P2X2/3 showed that this toxin is a highly selective blocker of exclusively P2X3 receptors. The selectivity
of action of PT1 demonstrated in our experiments shows that it is a unique agent, which opens up new prospects in the studies
of structural/functional peculiarities of receptor-channel complexes P2X3 as a peripheral link of the nociception system. 相似文献
34.
35.
Zaika O Mamenko M O'Neil RG Pochynyuk O 《American journal of physiology. Renal physiology》2011,300(5):F1105-F1115
Activation of the renal kallikrein-kinin system results in natriuresis and diuresis, suggesting its possible role in renal tubular sodium transport regulation. Here, we used patch-clamp electrophysiology to directly assess the effects of bradykinin (BK) on the epithelial Na(+) channel (ENaC) activity in freshly isolated split-opened murine aldosterone-sensitive distal nephrons (ASDNs). BK acutely inhibits ENaC activity by reducing channel open probability (P(o)) in a dose-dependent and reversible manner. Inhibition of B2 receptors with icatibant (HOE-140) abolished BK actions on ENaC. In contrast, activation of B1 receptors with the selective agonist Lys-des-Arg(9)-BK failed to reproduce BK actions on ENaC. This is consistent with B2 receptors playing a critical role in mediating BK signaling to ENaC. BK has little effect on ENaC P(o) when G(q/11) was inhibited with Gp antagonist 2A. Moreover, inhibition of phospholipase C (PLC) with U73122, but not saturation of cellular cAMP levels with the membrane-permeable nonhydrolysable cAMP analog 8-cpt-cAMP, prevents BK actions on ENaC activity. This argues that BK stimulates B2 receptors with subsequent activation of G(q/11)-PLC signaling cascade to acutely inhibit ENaC activity. Activation of BK signaling acutely depletes apical PI(4,5)P(2) levels. However, inhibition of Ca(2+) pump SERCA of the endoplasmic reticulum with thapsigargin does not prevent BK signaling to ENaC. Furthermore, caffeine, while producing a similar rise in [Ca(2+)](i) as in response to BK stimulation, fails to recapitulate BK actions on ENaC. Therefore, we concluded that BK acutely inhibits ENaC P(o) in mammalian ASDN via stimulation of B2 receptors and following depletion of PI(4,5)P(2), but not increases in [Ca(2+)](i). 相似文献
36.
Natural selection causing microsatellite divergence in wild emmer wheat at the ecologically variable microsite at Ammiad, Israel 总被引:10,自引:0,他引:10
Y. -C. Li M. S. Röder T. Fahima V. M. Kirzhner A. Beiles A. B. Korol E. Nevo 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2000,100(7):985-999
Genetic diversity at 28 microsatellite loci was studied in a natural population of Triticum dicoccoides at the Ammiad microsite, north of the Sea of Galilee, Israel. This microsite was subdivided into four major habitats, North,
Valley, Ridge and Karst, and further subdivided into nine subhabitats. The units thus defined showed strong and highly significant
differentiation in ecological factors; in particular with respect to cover, proximity and height of rocks, and surface soil
moisture after early rains. The results showed that allele distributions at microsatellite loci were nonrandom and associated with habitats. Significant genetic differentiation and variation in repeat number were found among subpopulations
in the four major habitats and nine subhabitats. Habitat-specific and -unique alleles and linkage disequilibria were observed in the Karst subpopulation. The subpopulations dwelling in drier habitats
and subhabitats showed higher genetic diversities at microsatellite loci. These results suggest that natural selection, presumably
through aridity stress, acts upon microsatellite divergence predominantly on noncoding sequences, thereby contributing to
differences in fitness.
Received: 9 September 1999 / Accepted: 16 September 1999 相似文献
37.
38.
This paper presents a comparison of three methods of parameter estimation in analysis of linkage between a quantitative trait locus (QTL) and a marker locus: maximum likelihood, mean square for trait cumulative distribution function, and method of moments, employing simulated backcross data. The sensitivity of estimates to violation of assumptions of normality and equal variances were also studied. Some measures of discrepancy between the trait distributions in the QTL groups are considered to evaluate the potential dependence of the resolution capacity of the QTL substitution effect with respect to trait mean value and variance. 相似文献
39.
Abraham B. Korol Valery M. Kirzhner Yeafim I. Ronin Eviatar Nevo 《Evolution; international journal of organic evolution》1996,50(4):1432-1441
The subject of this paper is polymorphism maintenance due to stabilizing selection with a moving optimum. It was shown that in case of two-locus additive control of the selected trait, global polymorphism is possible only when the geometric mean fitnesses of double homozygotes averaged over the period are lower than that of the single heterozygotes and of the double heterozygote (with a multiplier [1 – r]p, which depends on recombination rate r and period length p). But local stability of polymorphism cannot be excluded even if geometric mean fitnesses of all double homozygotes are higher than that of all heterozygotes. We proved, that for logarithmically convex fitness functions, cyclical changes of the optimum cannot help in polymorphism maintenance in case of additive control of the selected trait by two equal loci. However, within the same class of fitness functions, nonequal gene action and/or dominance effect for one or both loci may lead to local polymorphism stability with large enough polymorphism attracting domain. The higher the intensity of selection and closer the linkage between selected loci the larger is this domain. Note that even simple cyclical selection could result in two forms of polymorphic limiting behavior: (a) usually expected forced cycle with a period equal to that of environmental changes; and (b) “supercycles,” nondumping auto-oscillations with a period comprising of hundreds of forced oscillation periods. 相似文献
40.
Identification of molecular markers linked to the Yr15 stripe rust resistance gene of wheat originated in wild emmer wheat, Triticum dicoccoides 总被引:11,自引:0,他引:11
G. L. Sun T. Fahima A. B. Korol T. Turpeinen A. Grama Y. I. Ronin E. Nevo 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》1997,95(4):622-628
The Yr15 gene of wheat confers resistance to the stripe rust pathogen Puccinia striiformis West., which is one of the most devastating diseases of wheat throughout the world. In the present study, molecular markers
flanking the Yr15 gene of wheat have been identified using the near-isogenic-lines approach. RFLP screening of 76 probe-enzyme combinations
revealed one polymorphic marker (Nor/TaqI) between the susceptible and the resistant lines. In addition, out of 340 RAPD primers tested, six produced polymorphic
RAPD bands between the susceptible and the resistant lines. The genetic linkage of the polymorphic markers was tested on segregating
F2 population (123 plants) derived from crosses between stripe rust-susceptible Triticum durum wheat, cv D447, and a BC3F9 resistant line carrying Yr15 in a D447 background. A 2.8-kb fragment produced by the Nor RFLP probe and a 1420-bp PCR product generated by the RAPD primer OPB13 showed linkage, in coupling, with the Yr15 gene. Employing the standard maximum-likelihood technique it was found that the order OPB13
1420
–Yr15–Nor1 on chromosome 1B appeared to be no less than 1000-times more probable than the closest alternative. The map distances between
OPB13
1420
–Yr15–Nor1 are 27.1 cM and 11.0 cM for the first and second intervals, respectively. The application of marker-assisted selection for
the breeding of new wheat cultivars with the stripe rust resistance gene is discussed.
Received: 27 February 1997/Accepted: 7 March 1997 相似文献