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排序方式: 共有270条查询结果,搜索用时 0 毫秒
71.
72.
Kim MK Park SH Cho HD Cho SJ Kim A Kim HK Yeom BW Choi JS Kim CH 《Acta cytologica》2001,45(3):459-464
BACKGROUND: Primary pulmonary paragangliomas are rare tumors. To our knowledge, there is no prior report on fine needle aspiration cytology (FNAC) in pulmonary paraganglioma. CASE: A 34-year-old man presented with an incidentally found solitary pulmonary mass. FNAC showed papillarylike clusters of epithelioid cells with round to oval nuclei, evenly dispersed chromatin, micronucleoli and occasional anisonucleosis. These cytologic features were suggestive of a sclerosing hemangioma or bronchioloalveolar carcinoma. A right lower lobectomy revealed a primary pulmonary paraganglioma. CONCLUSION: The possibility of pulmonary paraganglioma should be considered in the differential diagnosis of FNAC showing pseudopapillary clusters of epithelioid cells. 相似文献
73.
Cheon YP Kim SW Kim SJ Yeom YI Cheong C Ha KS 《Biochemical and biophysical research communications》2000,273(3):997-1002
We have investigated a new role of RhoA in the germinal vesicle breakdown (GVBD) of mouse oocytes. First, RhoA was identified by immunostaining and ADP-ribosylation in germinal vesicle (GV) stage-oocytes. RhoA was mainly localized in the ooplasmic area, but rarely detected in germinal vesicle. Incubation of oocyte extract with C3 transferase induced a strong ADP-ribosylation at about 25 kDa. Incubation of GV-stage oocytes in culture medium induced the spontaneous maturation to GVBD by about 78 and 87% of total oocytes at 1 and 3 h, respectively. However, microinjection of C3 transferase into GV-stage oocytes significantly inhibited GVBD at 1 (GVBD = 29%) and 3 h (GVBD = 49%). To study the role of reactive oxygen species (ROS) in the oocyte maturation, the level of intra-oocyte ROS was measured using a ROS-specific fluorescent dye H(2)DCFDA during the oocyte maturation. Spontaneous maturation of GV-stage oocytes induced a significant increase of ROS at 3 h by about twofold over the control level and then the increased level was maintained until 6 h. However, microinjection of C3 transferase inhibited the production of intra-oocyte ROS. Incubation with ROS scavengers, N-acetyl-l-cysteine and catalase, blocked the ROS increase. The ROS scavengers also significantly inhibited GVBD, as did C3 transferase. Thus, it was proposed that RhoA was involved in the GVBD, possibly by the production of ROS in mouse oocytes. 相似文献
74.
A continuous treatment system combining a packed-bed column and a two-phase partitioning bioreactor has been designed to treat high-concentration benzene-containing gas streams. 1-Octadecene was used in a closed loop as an absorbant to scrub benzene in the counter-current column, after which it was transferred to the two-phase partitioning bioreactor to partition benzene into the 1 l aqueous phase for degradation by Klebsiella sp. The solvent was then recirculated back to the absorber. A gas stream containing 20 mg l–1 benzene at a flow rate of 60 l h–1 was introduced to the system, and the benzene was degraded at a biological removal efficiency of 87% at steady state. 相似文献
75.
Regulation of hypoxia responses by flavin adenine dinucleotide-dependent modulation of HIF-1α protein stability 下载免费PDF全文
76.
Purpose
Human sodium/iodide symporter (hNIS) protein is a membrane glycoprotein that transports iodide ions into thyroid cells. The function of this membrane protein is closely regulated by post-translational glycosylation. In this study, we measured glycosylation-mediated changes in subcellular location of hNIS and its function of iodine uptake.Methods
HeLa cells were stably transfected with hNIS/tdTomato fusion gene in order to monitor the expression of hNIS. Cellular localization of hNIS was visualized by confocal microscopy of the red fluorescence of tdTomato. The expression of hNIS was evaluated by RT-PCR and immunoblot analysis. Functional activity of hNIS was estimated by radioiodine uptake. Cyclic AMP (cAMP) and tunicamycin were used to stimulate and inhibit glycosylation, respectively. In vivo images were obtained using a Maestro fluorescence imaging system.Results
cAMP-mediated Glycosylation of NIS resulted in increased expression of hNIS, stimulating membrane translocation, and enhanced radioiodine uptake. In contrast, inhibition of glycosylation by treatment with tunicamycin dramatically reduced membrane translocation of intracellular hNIS, resulting in reduced radioiodine uptake. In addition, our hNIS/tdTomato fusion reporter successfully visualized cAMP-induced hNIS expression in xenografted tumors from mouse model.Conclusions
These findings clearly reveal that the membrane localization of hNIS and its function of iodine uptake are glycosylation-dependent, as our results highlight enhancement of NIS expression and glycosylation with subsequent membrane localization after cAMP treatment. Therefore, enhancing functional NIS by the increasing level of glycosylation may be suggested as a promising therapeutic strategy for cancer patients who show refractory response to conventional radioiodine treatment. 相似文献77.
Jang-Won Ahn Sunjik Kim Wooju Na Su-Jin Baek Jeong-Hwan Kim Keehong Min Jeonghun Yeom Hoyun Kwak Sunjoo Jeong Cheolju Lee Seon-Young Kim Cheol Yong Choi 《Nucleic acids research》2015,43(13):6321-6333
DNA double-strand breaks (DSBs) are the most severe type of DNA damage and are primarily repaired by non-homologous end joining (NHEJ) and homologous recombination (HR) in the G1 and S/G2 phase, respectively. Although CtBP-interacting protein (CtIP) is crucial in DNA end resection during HR following DSBs, little is known about how CtIP levels increase in an S phase-specific manner. Here, we show that Serpine mRNA binding protein 1 (SERBP1) regulates CtIP expression at the translational level in S phase. In response to camptothecin-mediated DNA DSBs, CHK1 and RPA2 phosphorylation, which are hallmarks of HR activation, was abrogated in SERBP1-depleted cells. We identified CtIP mRNA as a binding target of SERBP1 using RNA immunoprecipitation-coupled RNA sequencing, and confirmed SERBP1 binding to CtIP mRNA in S phase. SERBP1 depletion resulted in reduction of polysome-associated CtIP mRNA and concomitant loss of CtIP expression in S phase. These effects were reversed by reconstituting cells with wild-type SERBP1, but not by SERBP1 ΔRGG, an RNA binding defective mutant, suggesting regulation of CtIP translation by SERBP1 association with CtIP mRNA. These results indicate that SERBP1 affects HR-mediated DNA repair in response to DNA DSBs by regulation of CtIP translation in S phase. 相似文献
78.
Khac-Uan Do J. Rajesh Banu S. Kaliappan Ick-Tae Yeom 《Biotechnology and Bioprocess Engineering》2013,18(2):313-320
In the present study, a laboratory scale anoxic/oxic (A/O) reactor is used for the removal of nutrient and sludge reduction. Phosphorus removal was achieved through simultaneous precipitation, and sludge production was reduced through thermochemical pretreatment. The main objective of the study was to investigate the influence of sludge pretreatment on the nitrification rate. Total phosphorus in the effluent was maintained around 0.5 ~ 1.0 mg/L by simultaneous precipitation, using coagulant alum at 2.2 mole ratio. Before simultaneous precipitation, the nitrification rate of the A/O reactor was found to be 0.050 g N-NH4 +/g MLVSS.d. The thermochemical sludge pretreatment began on the 120th day at pH 11 and 80°C. The initiation of sludge pretreatment brought about a significant reduction of the A/O reactor nitrification rate, which fell to 0.038 g N-NH4 +/g MLVSS/day. The effect of sludge pretreatment was reflected in the reduction of the nitrogen removal efficiency from 85 to 74%. Recycling of the thermochemically pretreated sludge accounted for 57% sludge reduction, which had an adverse influence on the nitrification rate of the system. 相似文献
79.
The explosion of data and transactions demands a creative approach for data processing in a variety of applications. Research
on remote memory systems (RMSs), so as to exploit the superior characteristics of dynamic random access memory (DRAM), has
been performed for many decades, and today’s information explosion galvanizes researchers into shedding new light on the technology.
Prior studies have mainly focused on architectural suggestions for such systems, highlighting different design rationale.
These studies have shown that choosing the appropriate applications to run on an RMS is important in fully utilizing the advantages
of remote memory. This article provides an extensive performance evaluation for various types of data processing applications
so as to address the efficacy of an RMS by means of a prototype RMS with reliability functionality. The prototype RMS used
is a practical kernel-level RMS that renders large memory data processing feasible. The abstract concept of remote memory
was materialized by borrowing unused local memory in commodity PCs via a high speed network capable of Remote Direct Memory
Access (RDMA) operations. The prototype RMS uses remote memory without any part of its computation power coming from remote
computers. Our experimental results suggest that an RMS can be practical in supporting the rigorous demands of commercial
in memory database systems that have high data access locality. Our evaluation also convinces us of the possibility that a
reliable RMS can satisfy both the high degree of reliability and efficiency for large memory data processing applications
whose data access pattern has high locality. 相似文献
80.
Yoon Joo Young Yeom Jeonghun Lee Heebum Kim Kyutae Na Seungjin Park Kunsoo Paek Eunok Lee Cheolju 《BMC bioinformatics》2011,12(1):1-12