全文获取类型
收费全文 | 10802篇 |
免费 | 931篇 |
国内免费 | 902篇 |
出版年
2024年 | 29篇 |
2023年 | 138篇 |
2022年 | 243篇 |
2021年 | 477篇 |
2020年 | 355篇 |
2019年 | 439篇 |
2018年 | 502篇 |
2017年 | 345篇 |
2016年 | 491篇 |
2015年 | 692篇 |
2014年 | 823篇 |
2013年 | 898篇 |
2012年 | 1033篇 |
2011年 | 904篇 |
2010年 | 593篇 |
2009年 | 417篇 |
2008年 | 555篇 |
2007年 | 458篇 |
2006年 | 406篇 |
2005年 | 372篇 |
2004年 | 358篇 |
2003年 | 361篇 |
2002年 | 281篇 |
2001年 | 182篇 |
2000年 | 148篇 |
1999年 | 172篇 |
1998年 | 95篇 |
1997年 | 68篇 |
1996年 | 55篇 |
1995年 | 68篇 |
1994年 | 61篇 |
1993年 | 42篇 |
1992年 | 59篇 |
1991年 | 51篇 |
1990年 | 34篇 |
1989年 | 32篇 |
1988年 | 36篇 |
1987年 | 19篇 |
1986年 | 27篇 |
1985年 | 23篇 |
1984年 | 25篇 |
1983年 | 15篇 |
1982年 | 14篇 |
1981年 | 14篇 |
1979年 | 12篇 |
1974年 | 12篇 |
1973年 | 21篇 |
1972年 | 12篇 |
1966年 | 12篇 |
1958年 | 13篇 |
排序方式: 共有10000条查询结果,搜索用时 15 毫秒
121.
122.
123.
Jiřina Dvořáková 《Biologia Plantarum》1979,21(5):372-375
The capability of utilizing 20 amino acids and 2 amides as the sole nitrogen source for growth was studied in two green algae
(Chlorophyceae). A comparison was made of the growth rate of algae in a mineral nutrient solution containing nitrate as the nitrogen source,
with that in the same solution in which nitrogen in the form of nitrate was substituted by an equivalent nitrogen amount in
the form of various amino acids. In addition to this, another series of experiments was carried out in whioh both culture
media were supplied with glucose. The results show that both algae utilize a series of amino acids in dependence of their
structure (mostly 3-carbon amino acids). The growth rate ofChlorella in the presence of these sources is the same as in nitrate, that ofScenedesmus even much higher. In the cultures containing glucose both algal species exhibit a higher growth rate in the media with the
nitrate nitrogen source than in those with amino acids (with the exception of glycine inScenedesmus). 相似文献
124.
Barley seeds were treated for 3 h at 25°C with 240 mM ethyl methanesulphonate (EMS), washed for 18 h, treated with various concentrations of unbuffered sodium azide (pH 6.7–7.3) for 3 h at 25°C, re-dried to 30% water content and either sown immediately or stored at 25°C for 12 days and then sown. The synergistic action of sodium azide post-treatment has been demonstrated only for the EMS-induced M1 germination reduction, while the EMS-induced M1 sterility and the yield of M2 chlorophyll mutants were unaffected. The ?storage” recovery from EMS-induced mutagenic effects was insensitive to sodium azide post-treatment. The 12 day-seed storage at 25°C brought about an improvement of M1 germination, M1 survival, M1 fertility and a decrease in the amount of M2 mutants, regardless of whether sodium azide post-treatment was applied or not. 相似文献
125.
Summary A population sample of 416 unrelated donors from eské Budjovice (southorn Bohemia) was investigated for the phenotypes of phosphoglucomutase (PGM). The calculated frequencies of the allelles PGM
1
1
and PGM
1
2
, 0.770 and 0.230, respectively, correspond to the expected frequencies of the phenotypes PGM 1=0.593, PGM 2-1=0.354, and PGM 2=0.0529. No rare phonotype was detected. 相似文献
126.
Jiří Váňa 《Folia Geobotanica》1975,10(3):277-323
Die achte Studie über die SubfamilieJungermannioideae (Jungermanniaceae, Hepaticae) ist dén Arten der GattungJungermannia L. emend.Dum., die in Australien, Neuseeland und Ozeanien vorkommen, gewidmet. Die meist endemischen Arten, die auf Hawaii vorkommen, werden als eine selbständige (neunte) Studie bearbeitet. Im genannten Gebiet wurden 13 Arten der GattungJungermannia L. emend.Dum. festgestellt.J. (P.) hasskarliana (Nees) Steph.,J. (P.) obliquifolia (Schiffn.) Váňa,J. (P.) tetragona Lindenb.,J. (P.) hirticalyx Steph.,J. (P.) minutiverrucosa Amak.,J. (P.) wattsiana Steph.,J. (S.) ariadne Tayl.,J. (S.) totipapillosa Hodgs.,J. (S.) inundata Hook. fil. etTayl. emend.Mitt. undJ. (S.) orbiculata (Col.) Grolle sind eingehend taxonomisch bearbeitet,J. (P.) micrantha (Mitt.) Steph. wird in die nächste Studie eingereiht.J. (P.) boninensis (Horik.) Inoue wurde vonInoue (Inoue etIwatsuki 1969) eingehend beschrieben und abgebildet,Haplozia comptonii Pears. wurde schon im 2. Teil diskutiert. Die anderen aus dem Gebiet bekannten Arten sind mit den obenerwähnten Arten synonymisiert oder zu anderen Gattungen gestellt. 相似文献
127.
128.
W. F. Li J. Ji G. Wang H. Y. Wang B. L. Niu T. L. Josine 《Russian Journal of Genetics》2011,47(9):1039-1046
Many natural proteins have been developed into drugs and produced for direct application. Identifying improved hosts to achieve
high-level heterologous protein production is a challenge in the study of heterologous protein expression in recombinant yeast.
In this study, a novel high-throughput assay to screen such overproducing Saccharomyces cerevisiae strains was systematically developed. The protocol designed was based on screening host strain derivatives with increased
superoxide dismutase dependent resistance to oxidative stress. Yeast cells transformed with recombinant plasmid carrying SOD1 gene as a reporter responded exquisitely to oxidative stress induced by elevated concentrations of paraquat. Improved yeast
strains resulting from screening clones subjected to genome shuffling through selective pressure argue for a more effective
screening system compared with traditonal selection. Moreover, this approach can be employed in general biochemical analysis
without utilization of flow cytometry or well plate reader. Therefore, it is expected that the high-throughput assay would
make superior strains producing heterologous proteins. 相似文献
129.
130.
A simple and efficient tool to isolate epithelial cells from bacteria-contaminated samples has been developed using two different microparticles functionalized with chemical molecules. The epithelial cells could be captured simply by biocompatible anchors for membranes (BAM), consisting of poly(ethylene glycol) functionalized with oleyl-chain-conjugated NHS (N-hydroxysuccinimide) on glass microparticles, whereas bacteria were adsorbed on 3-aminopropyltrimethoxysilane (ATPS)-functionalized magnetic microparticles. In the case of samples highly contaminated with bacteria, epithelial cells were not isolated successfully by both of the single BAM- and antibody-functionalized microparticles. Therefore, serial isolation steps of these two different chemical functionalized microparticles were introduced. The concentration of bacteria was decreased dramatically by using APTS-functionalized magnetic particles prior to the isolation of epithelial cells by BAM microparticles. With these serial processes, successful isolation of epithelial cells was achieved from bacteria-contaminated epithelial samples. The applicability of this method was verified with bacteria-contaminated intestinal samples biopsied from a BALB/C mouse for primary cell cultivation. 相似文献