全文获取类型
收费全文 | 404篇 |
免费 | 12篇 |
国内免费 | 1篇 |
出版年
2022年 | 1篇 |
2021年 | 3篇 |
2020年 | 5篇 |
2019年 | 4篇 |
2018年 | 1篇 |
2017年 | 8篇 |
2016年 | 7篇 |
2015年 | 15篇 |
2014年 | 18篇 |
2013年 | 19篇 |
2012年 | 32篇 |
2011年 | 21篇 |
2010年 | 20篇 |
2009年 | 29篇 |
2008年 | 25篇 |
2007年 | 29篇 |
2006年 | 20篇 |
2005年 | 22篇 |
2004年 | 26篇 |
2003年 | 25篇 |
2002年 | 21篇 |
2001年 | 10篇 |
2000年 | 11篇 |
1999年 | 9篇 |
1998年 | 4篇 |
1997年 | 2篇 |
1996年 | 7篇 |
1994年 | 2篇 |
1993年 | 1篇 |
1992年 | 2篇 |
1991年 | 3篇 |
1990年 | 2篇 |
1989年 | 2篇 |
1988年 | 1篇 |
1987年 | 2篇 |
1986年 | 1篇 |
1985年 | 1篇 |
1968年 | 1篇 |
1964年 | 1篇 |
1961年 | 3篇 |
1958年 | 1篇 |
排序方式: 共有417条查询结果,搜索用时 15 毫秒
81.
Mutsumi Imai Michiko Miyazaki H. Henny Yeung Shohei Hidaka Katerina Kantartzis Hiroyuki Okada Sotaro Kita 《PloS one》2015,10(2)
Sound symbolism, or the nonarbitrary link between linguistic sound and meaning, has often been discussed in connection with language evolution, where the oral imitation of external events links phonetic forms with their referents (e.g., Ramachandran & Hubbard, 2001). In this research, we explore whether sound symbolism may also facilitate synchronic language learning in human infants. Sound symbolism may be a useful cue particularly at the earliest developmental stages of word learning, because it potentially provides a way of bootstrapping word meaning from perceptual information. Using an associative word learning paradigm, we demonstrated that 14-month-old infants could detect Köhler-type (1947) shape-sound symbolism, and could use this sensitivity in their effort to establish a word-referent association. 相似文献
82.
T2BP, a novel TRAF2 binding protein, can activate NF-kappaB and AP-1 without TNF stimulation. 总被引:2,自引:0,他引:2
Mutsumi Kanamori Harukazu Suzuki Rintaro Saito Masami Muramatsu Yoshihide Hayashizaki 《Biochemical and biophysical research communications》2002,290(3):1108-1113
TRAF2 is a key molecule involved in TNF signaling, which is crucial for the regulation of inflammatory processes. We have identified a novel TRAF2 binding protein, designated as T2BP (TRAF2 binding protein), by a mammalian two-hybrid screening approach. T2BP is a relatively small protein of 184 amino acids, which includes a forkhead-associated domain, the phosphopeptide binding motif. The interaction domain search showed that the TRAF domain in TRAF2 is required for the binding to T2BP whereas almost the entire protein in T2BP binds to TRAF2. The interaction was further confirmed by co-immunoprecipitation. Expression profiling for T2BP and TRAF2 revealed an ubiquitous expression in adult mouse tissues. Overexpression of T2BP in HEK293 cells activated NF-kappaB and AP-1 in a dose dependent manner as well as seen in the TNF-treated control cells. Our results suggest that T2BP is involved in the TNF-mediated signaling by its interaction with TRAF2. 相似文献
83.
84.
Mutsumi Miyauchi Takashi Takata Ikuko Ogawa Hiroshi Ito Joji Kobayashi Hirosmasa Nikai Naokuni Ijuhin 《Histochemistry and cell biology》1996,105(1):27-31
To demonstrate the tissue localization of prostaglandin (PG) E2, PGF2 and 6-keto-PGF1 (a stable metabolite of PGI2) various tissues, including decalcified periodontal tissue of 7-week-old male Wistar strain rats, were immunohistochemically examined using a streptavidin-biotin complex method. Besides tissue macrophages and endothelial cells in various tissues, hepatocytes, renal tubular cells, and parietal and chief cells in the gastric mucosa showed a positive reaction for the various PGs examined. PGs were demonstrated in the cytoplasm or in association with the cell membrane. We generally observed no difference between the localization patterns of PGE2-, PGF2-, and 6-keto-PGF1-positive cells in these tissues. However, in the periodontal ligament and alveolar bone, 6-keto-PGF1 was localized in the cytoplasm of osteocytes, osteoblasts, cementocytes, and cementoblasts, while no reaction for PGE2 or PGF2 was revealed in these cells. We demonstrated the immunohistochemical localization of PGs in various rat tissues including decalcified periodontal tissue and discuss the important roles of PGs in the modulation of their normal functions in these tissues. 相似文献
85.
86.
Territorial behaviour, reproduction and migration of the epilithic algal eater,Tropheus moorii, were investigated in Lake Tanganyika, Africa. Adults of both sexes had individual feeding territories which adjointed each
other. Males, who occupied higher rocks than females, usually stayed at the same sites for more than 5 months. Females left
their territories to pair with males in the males’ territories. Paired females actively foraged under the protection of their
mates for up to 3 weeks prior to spawning. After spawning, females usually settled in a site unoccupied by territory-holders
to mouthbrood the offspring for a month. An examination of the ovaries and a removal experiment of dominant males suggest
that females cannot attain fully mature ovaries in their own territories and choose males whose territories can provide enough
food to satisfy their nutritive demand. The evolution of a number of local colour morphs in this fish is briefly discussed
in relation to social selection. 相似文献
87.
88.
Chikayoshi Matsuda Mutsumi Takagi Takako Hattori Shigeyuki Wakitani Toshiomi Yoshida 《Cytotechnology》2005,47(1-3):11-17
A differentiation method of human bone marrow mesenchymal stem cells (MSCs) to chondrocytes was developed for the construction
of a three-dimensional (3D) cartilage tissue. The adhesive cells, which were isolated from a human bone marrow aspirate were
embedded in type I collagen in a poly-l-lactate-glycolic acid copolymer (PLGA) mesh and cultivated for 4 week together with growth factors. The degree of cellular
differentiation was estimated by quantitative RT-PCR of aggrecan and type II collagen mRNAs and by staining with Safranin
O. The 3D culture showed a higher degree of differentiation even without growth factors than the conventional pellet culture
with growth factors, namely, dexamethasone and transforming growth factor (TGF)-β 3. The 3D culture for 2 week with the combined
addition of dexamethasone, TGF-β 3, and insulin-like growth factor (IGF)-I reached a 30% expression of aggrecan mRNA compared
with that in primary human chondrocytes, while the aggrecan mRNA expression in the conventional pellet culture was less than
2%. The sequential two-step differentiation cultivation, during which the cells were cultivated in 3D for 1 week after the
conventional two-dimensional (2D) culture for 1 week, could markedly accelerate the expression of aggrecan mRNA compared with
the 3D cultivation for 2 week. 相似文献
89.
Kato N Akai M Zulkifli L Matsuda N Kato Y Goshima S Hazama A Yamagami M Guy HR Uozumi N 《Channels (Austin, Tex.)》2007,1(3):161-171
Studies suggest that Ktr/Trk/HKT-type transporters have evolved from multiple gene fusions of simple K(+) channels of the KcsA type into proteins that span the membrane at least eight times. Several positively charged residues are present in the eighth transmembrane segment, M2(D), in the transporters but not K(+) channels. Some models of ion transporters require a barrier to prevent free diffusion of ions down their electrochemical gradient, and it is possible that the positively charged residues within the transporter pore may prevent transporters from being channels. Here we studied the functional role of these positive residues in three Ktr/Trk/HKT-type transporters (Synechocystis KtrB-mediated K(+) uniporter, Arabidopsis AtHKT1-mediated Na(+) uniporter and wheat TaHKT1-mediated K(+)/Na(+) symporter) by examining K(+) uptake rates in E. coli, electrophysiological measurements in oocytes and growth rates of E. coli and yeast. The conserved Arg near the middle of the M2(D) segment was essential for the K(+) transport activity of KtrB and plant HKTs. Combined replacement of several positive residues in TaHKT1 showed that the positive residue at the beginning of the M2(D), which is conserved in many K(+) channels, also contributed to cation transport activity. This positive residue and the conserved Arg both face towards the ion conducting pore side. We introduced an atomic-scale homology model for predicting amino acid interactions. Based on the experimental results and the model, we propose that a salt bridge(s) exists between positive residues in the M2(D) and conserved negative residues in the pore region to reduce electrostatic repulsion against cation permeation caused by the positive residue(s). This salt bridge may help stabilize the transporter configuration, and may also prevent the conformational change that occurs in channels. 相似文献
90.
Masaru Nakano Hiroto Umehara Yoshihiro Hara Motohide Makino Mika Igarashi Mutsumi Nakada Toru Nakamura Yoichiro Hoshino Akira Kanno 《Molecular breeding : new strategies in plant improvement》2007,20(4):425-429
The class B genes, which belong to the MADS-box gene family, play important roles in regulating petal and stamen development
in flowering plants. These genes exist in two different types termed DEF- and GLO-like genes, and the B-function is provided by heterodimers of a DEF- and a GLO-like gene product. In the present study, dicot (tobacco and lettuce) and monocot (Tricyrtis hirta) plants were transformed with the GLO-like gene of Agapanthus praecox ssp. orientalis
ApGLO alone or in combination with the DEF-like gene of the same plant ApDEF. In two out of 10 transgenic tobacco plants containing ApGLO, sepals partially converted into petaloid organs. For lettuce, ray florets of four out of nine transgenic plants containing
ApGLO also developed additional petaloid organs. In two out of five transgenic T. hirta plants containing both ApGLO and ApDEF, organs developed in whorl 4 showed noticeable morphological alteration: they were much longer compared with carpels of non-transgenic
plants, and had purple spots overall on the surface as filaments of non-transgenic plants. No morphological alterations were
observed in vegetative organs between transgenic and non-transgenic plants for all the three species. The results obtained
in the present study indicate a possibility of molecular breeding for flower form alteration by genetic transformation with
the class B MADS-box gene(s) of heterologous plant species. 相似文献