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951.
952.
The mutagenic specificity of sodium bisulfite 总被引:5,自引:0,他引:5
F Mukai I Hawryluk R Shapiro 《Biochemical and biophysical research communications》1970,39(5):983-988
953.
Detection of B virus infection in cynomolgus monkeys by ELISA using simian agent 8 as alternative antigen. 总被引:4,自引:0,他引:4
The use of simian agent 8 (SA8) as an antigen for B virus (BV) antibody detection was evaluated in cynomolgus monkeys. Seventy-two sera judged as positive using BV antigen were all positive when the SA8 antigen was used. Out of 28 BV-negative sera 2 were positive against the SA8 antigen and one serum was classified as indeterminate. The present data indicates that detection of BV antibody can be achieved accurately and safely by enzyme-linked immunosorbent assay (ELISA) using SA8 antigen. 相似文献
954.
955.
Repeated DNA sequences near the 5'-end of the silk fibroin gene 总被引:4,自引:0,他引:4
956.
Kazuo Mukai Seiji Kikuchi Shiro Urano 《Biochimica et Biophysica Acta (BBA)/General Subjects》1990,1035(1):77-82
Kinetic study of the reaction between tocopheroxyl (vitamin E radical) and reduced ubiquinone, n = 10) has been performed. The rates of reaction of ubiquinol with α-tocopheroxyl 1 and seven kinds of alkyl substituted tocopheroxyl radicals 2–8 in solution have been determined spectrophotometrically, using a stopped-flow technique. The result shows that the rate constants decrease as the total electron-donating capacity of the alkyl substituents on the aromatic ring of tocopheroxyls increases. For the tocopheroxyls with two alkyl substituents at ortho positions (C-5 and C-7), the second-order rate constants, k1, obtained vary i n the order of 102, and decrease predominantly, as the size of two ortho-alkyl groups (methyl, ethyl, isopropyl and tert-buty) in tocopheroxyl increases. On the other hand, the reaction between tocopheroxyl and ubiquinone-10 (oxidized ubiquinone) has not been observed. The result indicates that ubiquinol-10 regenerates tocopherol by donating a hydrogen atom of the 1-OH and/or 4-OH group to the tocopheroxyl radical. For instance, the k1 values obtained for α-tocopheroxyl are 3.74 · 105 M?1 · s?1 and 2.15 · 5 M?1 · s?1 in benzene and ethanol solution at 25°C, respectively. The above reaction rates, k1, obtained were compared with those of vitamin C with α-tocopheroxyl reported by Packer et al. (k2 = 1.55 · 106 M?1 · s?1) and Scarpa et al. (K2 = 2 · 105 105 M?1 · s?1), which is well known as a usual regeneration reaction of tocopheroxyl in biomembrane systems. The result suggests that ubiquinol-10 also regenerates the tocopheroxyl to tocopherol and prevents lipid peroxidation in various tissues and mitochondria. 相似文献
957.
958.
Isolation of a novel antibody, which precipitates ribonucleoprotein complex containing threonine tRNA from a patient with polymyositis 总被引:3,自引:0,他引:3
N Okada R Mukai F Harada T Kabashima Y Nakao K Yamane Y Ohshima K Sakamoto M Itoh H Kashiwagi 《European journal of biochemistry》1984,139(3):425-429
We have isolated and characterized a novel antibody from a patient with polymyositis, which precipitates threonine tRNA and an unknown small RNA molecule from 32P-labeled HeLa extract. Fingerprint analysis showed that the human threonine tRNA precipitated was nearly identical to the murine tRNA described by Harada [(1978) Seikagaku 50, 397-411]. Nucleotide analysis suggested that the other small RNA molecule might be transfer RNA. Since phenol extraction resulted in the loss of immunoprecipitability, the protein portion was presumed to be involved in the recognition of the antigen. Immunofluorescence staining of HeLa cells with the antibody clearly demonstrated that the antigen was located predominantly in the cytoplasm. 相似文献
959.
960.