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131.
Long‐distance seed dispersal influences many critical ecological processes by improving chances of gene flow and maintaining genetic diversity among plant populations. Accordingly, large‐scale movements by frugivores may have important conservation implications as they provide an opportunity for long‐distance seed dispersal. We studied movement patterns, resource tracking, and potential long‐distance seed dispersal by two species of Ceratogymna hornbills, the black‐casqued hornbill C. atrata, and the white‐thighed hornbill C. cylindricus, in lowland tropical forests of Cameroon. We determined fruiting phenology of 24 tree species important in hornbill diet at monthly intervals and compared these patterns to monthly hornbill census data. After capture and radio‐tagging of 16 hornbills, we used radio telemetry by vehicle and fixed wing aircraft to determine the extent of long‐distance movements. Hornbills exhibited up to 20‐fold changes in numbers in response to fruit availability in our 25 km2 study area. Also, hornbills made large‐scale movements up to 290 km, which are larger than any movement previously reported for large avian frugivores. Together, these observations provide direct evidence that hornbills are not resident and that hornbills track available fruit resources. Our results suggest that Ceratogymna hornbills embark on long‐distance movements, potentially dispersing seeds and contributing to rain forest regeneration and diversity. 相似文献
132.
A conserved neutralizing epitope on gp41 of human immunodeficiency virus type 1. 总被引:29,自引:16,他引:13 下载免费PDF全文
T Muster F Steindl M Purtscher A Trkola A Klima G Himmler F Rüker H Katinger 《Journal of virology》1993,67(11):6642-6647
Vaccination against human immunodeficiency virus type 1 (HIV-1) requires an immunogen which will elicit a protective immunity against viruses that show a high degree of genetic polymorphism. Therefore, the identification of neutralizing epitopes which are shared by many strains would be useful. In previous studies, we established a human monoclonal antibody (2F5) that neutralizes a variety of laboratory strains and clinical isolates of HIV-1. In the present report, we define the amino acid sequence Glu-Leu-Asp-Lys-Trp-Ala (ELDKWA) on the ectodomain of gp41 as the epitope recognized by this antibody. The sequence was found to be conserved in 72% of otherwise highly variable HIV-1 isolates. Escape mutants were not detected in cells infected with HIV-1 isolates MN and RF in the presence of antibody 2F5. Since sequence variability of neutralizing epitopes is considered to be a major obstacle to HIV-1 vaccine development, the conserved B-cell epitope described here is a promising candidate for inclusion in a vaccine against AIDS. 相似文献
133.
Quantification of functional connectivity in physiological networks is frequently performed by means of time-variant partial directed coherence (tvPDC), based on time-variant multivariate autoregressive models. The principle advantage of tvPDC lies in the combination of directionality, time variance and frequency selectivity simultaneously, offering a more differentiated view into complex brain networks. Yet the advantages specific to tvPDC also cause a large number of results, leading to serious problems in interpretability. To counter this issue, we propose the decomposition of multi-dimensional tvPDC results into a sum of rank-1 outer products. This leads to a data condensation which enables an advanced interpretation of results. Furthermore it is thereby possible to uncover inherent interaction patterns of induced neuronal subsystems by limiting the decomposition to several relevant channels, while retaining the global influence determined by the preceding multivariate AR estimation and tvPDC calculation of the entire scalp. Finally a comparison between several subjects is considerably easier, as individual tvPDC results are summarized within a comprehensive model equipped with subject-specific loading coefficients. A proof-of-principle of the approach is provided by means of simulated data; EEG data of an experiment concerning visual evoked potentials are used to demonstrate the applicability to real data. 相似文献
134.
Günther Gerisch Britta Schroth-Diez Annette Müller-Taubenberger Mary Ecke 《Biophysical journal》2012,103(6):1170-1178
In a motile eukaryotic cell, front protrusion and tail retraction are superimposed on each other. To single out mechanisms that result in front to tail or in tail to front transition, we separated the two processes in time using cells that oscillate between a full front and a full tail state. State transitions were visualized by total internal reflection fluorescence microscopy using as a front marker PIP3 (phosphatidylinositol [3,4,5] tris-phosphate), and as a tail marker the tumor-suppressor PTEN (phosphatase tensin homolog) that degrades PIP3. Negative fluctuations in the PTEN layer of the membrane gated a local increase in PIP3. In a subset of areas lacking PTEN (PTEN holes), PIP3 was amplified until a propagated wave was initiated. Wave propagation implies that a PIP3 signal is transmitted by a self-sustained process, such that the temporal and spatial profiles of the signal are maintained during passage of the wave across the entire expanse of the cell membrane. Actin clusters were remodeled into a ring along the perimeter of the expanding PIP3 wave. The reverse transition of PIP3 to PTEN was linked to the previous site of wave initiation: where PIP3 decayed first, the entry of PTEN was primed. 相似文献
135.
Rolf Frischknecht Christian Bauer Christof Bucher Linda Ager-Wick Ellingsen Lukas Gutzwiller Britta Heimbach René Itten Xun Liao Evangelos Panos Stephan Pfister Tobias Schmidt Valentin Stahel Philippe Stolz Peter Toggweiler Karin Treyer Jacques Villeneuve Andreas Wade Marcel Weil 《The International Journal of Life Cycle Assessment》2018,23(8):1716-1721
136.
Modelling biological invasions over homogeneous and inhomogeneous landscapes using level set methods
The establishment, spread and subsequent degradation of existing environments by invasive species is a worldwide problem affecting
native and agricultural ecosystems. The phenomenal cost to governments as a result of research and eradication or control
drives the need to understand invasion characteristics. In this paper we develop a method for modelling the boundary of an
invasion over time with model inputs being the initial distribution of the invasion and the speed at which the invasion front
moves over time. This speed function can depend on the topography of the ground cover and we consider examples of homogeneous
and inhomogeneous spread. The possibility of a long-distance dispersal event occurring is also considered. In particular,
examples of the spread of emergent weeds and weeds which favour creeks and river beds in New Zealand are presented. 相似文献
137.
W T Morgan J Alam V Deaciuc P Muster F M Tatum A Smith 《The Journal of biological chemistry》1988,263(17):8226-8231
Sn-protoporphyrin IX (SnPP), an inhibitor of heme oxygenase and a potential therapeutic agent for neonatal hyperbilirubinemia, is bound tightly by hemopexin. The apparent dissociation constant (Kd) at pH 7.4 is 0.25 +/- 0.15 microM, but estimation of the Kd for the SnPP-hemopexin complex is hampered by the fact that at physiological pH SnPP exists as monomers and dimers, both of which are bound by hemopexin. SnPP is readily displaced from hemopexin by heme (Kd less than 1 pM). The hemopexin-SnPP interaction, like that of heme-hemopexin, is dependent on the histidine residues of hemopexin. However, as expected from the differences in the coordination chemistries of tin and iron, the stability of the histidyl-metalloporphyrin complex is lower for SnPP-hemopexin than for mesoheme-hemopexin. Nevertheless, when SnPP binds to hemopexin, certain of the ligand-induced changes in the conformation of hemopexin which increase the affinity of the protein for its receptor are produced. Binding of SnPP produces the conformational change in hemopexin which protects the hinge region of hemopexin from proteolysis, but SnPP does not produce the characteristic increase in the ellipticity of hemopexin at 231 nm that heme does. Competition experiments confirmed that human serum albumin (apparent Kd = 4 +/- 2 microM) has a significantly lower affinity for SnPP than does hemopexin. Appreciable amounts of SnPP (up to 35% in adults and 20% in neonates) would be bound by hemopexin in the circulation, and the remainder of SnPP would be associated with albumin due to the latter's high concentration in serum. Essentially no non-protein-bound SnPP is present. Importantly, SnPP-hemopexin binds to the hemopexin receptor on mouse hepatoma cells with an affinity comparable to that of heme-hemopexin and treatment of the hepatoma cells with SnPP-hemopexin causes a rapid increase in the steady state level of heme oxygenase messenger RNA. These results show that hemopexin participates in the transport of SnPP to heme oxygenase and in its regulation by SnPP. 相似文献
138.
Wurm David J. Quehenberger Julian Mildner Julia Eggenreich Britta Slouka Christoph Schwaighofer Andreas Wieland Karin Lendl Bernhard Rajamanickam Vignesh Herwig Christoph Spadiut Oliver 《Applied microbiology and biotechnology》2018,102(2):667-676
Applied Microbiology and Biotechnology - Against the outdated belief that inclusion bodies (IBs) in Escherichia coli are only inactive aggregates of misfolded protein, and thus should be avoided... 相似文献
139.
Britta Schaffelke 《Journal of phycology》1999,35(6):1150-1157
Large Fucales are abundant on coastal coral reefs of the Great Barrier Reef, but are often limited by the availability of inorganic nutrients. Particle loads in these reef waters are high, which is generally perceived as detrimental for aquatic plants due to a reduction of light. Here, I provide evidence that several abundant Sargassum species supplement their nutrient supply with nutrients derived from the layer of particulate matter (PM) deposited on their thalli. In experiments involving removal or addition of PM, growth rates of Sargassum spp. were up to 180% higher when particles were present on the thalli. Tissue nitrogen and phosphorus levels of thalli with a surface PM layer were 10%–30% higher than those of thalli without PM. The amount of PM deposited in situ on thalli of five species of brown algae ranged from 0.6 to 0.9 g Corg ·g−1 dry weight alga, depending on the species' morphology. I suggest that a nutrient-rich diffusive boundary layer is created on the thallus surface by an epiphytic microbial community that remineralizes the particulate nutrients. When algal growth is nutrient limited, the use of particle-derived nutrients as a source alternative to nutrients in the water column may outweigh any potential adverse effects of the thallus particle layer. 相似文献
140.
Biocontrol of the Food-Borne Pathogens Listeria monocytogenes and Salmonella enterica Serovar Poona on Fresh-Cut Apples with Naturally Occurring Bacterial and Yeast Antagonists 下载免费PDF全文
Britta Leverentz William S. Conway Wojciech Janisiewicz Maribel Abadias Cletus P. Kurtzman Mary J. Camp 《Applied microbiology》2006,72(2):1135-1140
Fresh-cut apples contaminated with either Listeria monocytogenes or Salmonella enterica serovar Poona, using strains implicated in outbreaks, were treated with one of 17 antagonists originally selected for their ability to inhibit fungal postharvest decay on fruit. While most of the antagonists increased the growth of the food-borne pathogens, four of them, including Gluconobacter asaii (T1-D1), a Candida sp. (T4-E4), Discosphaerina fagi (ST1-C9), and Metschnikowia pulcherrima (T1-E2), proved effective in preventing the growth or survival of food-borne human pathogens on fresh-cut apple tissue. The contaminated apple tissue plugs were stored for up to 7 days at two different temperatures. The four antagonists survived or grew on the apple tissue at 10 or 25°C. These four antagonists reduced the Listeria monocytogenes populations and except for the Candida sp. (T4-E4), also reduced the S. enterica serovar Poona populations. The reduction was higher at 25°C than at 10°C, and the growth of the antagonists, as well as pathogens, increased at the higher temperature. 相似文献