全文获取类型
收费全文 | 1758篇 |
免费 | 77篇 |
出版年
2024年 | 4篇 |
2023年 | 16篇 |
2022年 | 33篇 |
2021年 | 60篇 |
2020年 | 49篇 |
2019年 | 50篇 |
2018年 | 49篇 |
2017年 | 56篇 |
2016年 | 76篇 |
2015年 | 80篇 |
2014年 | 96篇 |
2013年 | 120篇 |
2012年 | 131篇 |
2011年 | 138篇 |
2010年 | 90篇 |
2009年 | 70篇 |
2008年 | 98篇 |
2007年 | 105篇 |
2006年 | 89篇 |
2005年 | 77篇 |
2004年 | 71篇 |
2003年 | 51篇 |
2002年 | 47篇 |
2001年 | 18篇 |
2000年 | 17篇 |
1999年 | 18篇 |
1998年 | 14篇 |
1997年 | 5篇 |
1996年 | 5篇 |
1995年 | 3篇 |
1994年 | 3篇 |
1993年 | 3篇 |
1992年 | 3篇 |
1991年 | 4篇 |
1990年 | 6篇 |
1989年 | 3篇 |
1988年 | 6篇 |
1987年 | 3篇 |
1986年 | 3篇 |
1985年 | 6篇 |
1984年 | 4篇 |
1983年 | 3篇 |
1982年 | 3篇 |
1977年 | 3篇 |
1975年 | 4篇 |
1974年 | 5篇 |
1973年 | 11篇 |
1972年 | 3篇 |
1971年 | 5篇 |
1970年 | 5篇 |
排序方式: 共有1835条查询结果,搜索用时 140 毫秒
991.
Dilek Canakci Nabih Lolak Mustafa Durgun 《Journal of enzyme inhibition and medicinal chemistry》2019,34(1):110-116
In this study, a series of 10 novel copper (II) and silver complexes of 1,3-diaryltriazene-substituted sulfonamides was synthesised. All the synthesised ligands and their metal complexes were assessed for in vitro cytotoxicity against human colorectal adenocarcinoma (DLD-1), cervix carcinoma (HeLa), breast adenocarcinoma (MDA-MB-231), colon adenocarcinoma (HT-29), endometrial adenocarcinoma (ECC-1), prostate cancer (DU-145 and PC-3), normal embryonic kidney (HEK-293), normal prostate epithelium (PNT-1A), and normal retinal pigment epithelium (ARPE-19) cells. Most of the metal complexes from the series showed to be more active against all cancerous cells than the uncomplexed 1,3-diaryltriazene-substituted sulfonamides, and lower cytotoxic effects observed on normal cells. Most of the Cu (II) and Ag (I) metal complexes from the presented series showed high cytotoxic activity against HeLa cells with IC50 values ranging from 2.08 to >300?µM. Specifically, compound L3-Ag showed one of the highest cytotoxicity against all cancer cell lines with IC50 values between 3.30 to 16.18?µM among other tested compounds. 相似文献
992.
993.
Length–weight relationships of 20 fish species from Pahang River,Maran district,Pahang, Malaysia 下载免费PDF全文
A. R. Zulkafli M. N. A. Amal S. Shohaimi A. Mustafa A. H. Ghani S. Hashim M. I. Anuar M. P. Hasfairi 《Zeitschrift fur angewandte Ichthyologie》2015,31(2):409-410
This study reports the length–weight relationships (LWR) of 20 fish species from the Pahang River, Maran district, Pahang, Malaysia. The river supplies fishes mainly for local consumption and is a principal body of water for freshwater aquaculture in Malaysia. A total of 12 LWR of fishes were unknown to FishBase, and five new maximum lengths of fishes were identified from the study. 相似文献
994.
Sujan Timilsina Mustafa O. Jibrin Neha Potnis Gerald V. Minsavage Misrak Kebede Allison Schwartz Rebecca Bart Brian Staskawicz Claudine Boyer Gary E. Vallad Olivier Pruvost Jeffrey B. Jones Erica M. Goss 《Applied and environmental microbiology》2015,81(4):1520-1529
Four Xanthomonas species are known to cause bacterial spot of tomato and pepper, but the global distribution and genetic diversity of these species are not well understood. A collection of bacterial spot-causing strains from the Americas, Africa, Southeast Asia, and New Zealand were characterized for genetic diversity and phylogenetic relationships using multilocus sequence analysis of six housekeeping genes. By examining strains from different continents, we found unexpected phylogeographic patterns, including the global distribution of a single multilocus haplotype of X. gardneri, possible regional differentiation in X. vesicatoria, and high species diversity on tomato in Africa. In addition, we found evidence of multiple recombination events between X. euvesicatoria and X. perforans. Our results indicate that there have been shifts in the species composition of bacterial spot pathogen populations due to the global spread of dominant genotypes and that recombination between species has generated genetic diversity in these populations. 相似文献
995.
996.
Gokhan Bayramoglu Aysegul Bayramoglu Yılmaz Altuner Mustafa Uyanoglu Suat Colak 《Cytotechnology》2015,67(3):487-491
There is a very little information about the protective effect of lycopene (LYC) against hepatic ischemia–reperfusion injury. The present study was designed to examine the possible protective effect of the strong antioxidant and anti-inflammatory agent, LYC, on hepatic ischemia/reperfusion injury. For this purpose, rats were subjected to 45 min of hepatic ischemia followed by 60 min of reperfusion period. LYC at the doses of 2.5 and 5 mg/kg body weight (bw) were injected intraperitoneally, 60 min prior to ischemia. Upon sacrification, hepatic tissue samples were used for the measurement of catalase (CAT) activity and malondialdehyde (MDA) levels. Also, aspartate aminotransferase (AST), alanine aminotransferase (ALT) and lactate dehydrogenase (LDH) were assayed in serum samples. As a result of the use of LYC at the doses of 2.5 and 5 mg/kg bw; while improvements of the ALT, AST, LDH and MDA values were partial and dose-dependent, the improvement of CAT activity was total and dose-independent (p < 0.05). Our findings suggest that LYC has a protective effect against ischemia/reperfusion injury on the liver. 相似文献
997.
Arne H. Eide Hasheem Mannan Mustafa Khogali Gert van Rooy Leslie Swartz Alister Munthali Karl-Gerhard Hem Malcolm MacLachlan Karin Dyrstad 《PloS one》2015,10(5)
There is an increasing awareness among researchers and others that marginalized and vulnerable groups face problems in accessing health care. Access problems in particular in low-income countries may jeopardize the targets set by the United Nations through the Millennium Development Goals. Thus, identifying barriers for individuals with disability in accessing health services is a research priority. The current study aimed at identifying the magnitude of specific barriers, and to estimate the impact of disability on barriers for accessing health care in general. A population based household survey was carried out in Sudan, Namibia, Malawi, and South Africa, including a total of 9307 individuals. The sampling strategy was a two-stage cluster sampling within selected geographical areas in each country. A listing procedure to identify households with disabled members using the Washington Group six screening question was followed by administering household questionnaires in households with and without disabled members, and questionnaires for individuals with and without disability. The study shows that lack of transport, availability of services, inadequate drugs or equipment, and costs, are the four major barriers for access. The study also showed substantial variation in perceived barriers, reflecting largely socio-economic differences between the participating countries. Urbanity, socio-economic status, and severity of activity limitations are important predictors for barriers, while there is no gender difference. It is suggested that education reduces barriers to health services only to the extent that it reduces poverty. Persons with disability face additional and particular barriers to health services. Addressing these barriers requires an approach to health that stresses equity over equality. 相似文献
998.
Tahir Atik Huseyin Onay Ayca Aykut Guney Bademci Tayfun Kirazli Mustafa Tekin Ferda Ozkinay 《PloS one》2015,10(11)
Comprehensive genetic testing has the potential to become the standard of care for individuals with hearing loss. In this study, we investigated the genetic etiology of autosomal recessive nonsyndromic hearing loss (ARNSHL) in a Turkish cohort including individuals with cochlear implant, who had a pedigree suggestive of an autosomal recessive inheritance. A workflow including prescreening of GJB2 and a targeted next generation sequencing panel (Illumına TruSightTM Exome) covering 2761 genes that we briefly called as mendelian exome sequencing was used. This panel includes 102 deafness genes and a number of genes causing Mendelian disorders. Using this approach, we identified causative variants in 21 of 29 families. Three different GJB2 variants were present in seven families. Remaining 14 families had 15 different variants in other known NSHL genes (MYO7A, MYO15A, MARVELD2, TMIE, DFNB31, LOXHD1, GPSM2, TMC1, USH1G, CDH23). Of these variants, eight are novel. Mutation detection rate of our workflow is 72.4%, confirming the usefulness of targeted sequencing approach in NSHL. 相似文献
999.
Reem A. Mustafa Wojtek Wiercioch Nancy Santesso Adrienne Cheung Barbara Prediger Tejan Baldeh Alonso Carrasco-Labra Romina Brignardello-Petersen Ignacio Neumann Patrick Bossuyt Amit X. Garg Monika Lelgemann Diedrich Bühler Jan Brozek Holger J. Schünemann 《PloS one》2015,10(10)
Objective
To develop guidance on what information to include and how to present it in tables summarizing the evidence from systematic reviews of test accuracy following the Grading of Recommendations Assessment, Development and Evaluation (GRADE) approach.Methods
To design and refine the evidence tables, we used an iterative process based on the analysis of data from four rounds of discussions, feedback and user testing. During the final round, we conducted one-on-one user testing with target end users. We presented a number of alternative formats of evidence tables to participants and obtained information about users’ understanding and preferences.Results
More than 150 users participated in initial discussions and provided their formal and informal feedback. 20 users completed one-on-one user testing interviews. Almost all participants preferred summarizing the results of systematic reviews of test accuracy in tabular format rather than plain text. Users generally preferred less complex tables but found presenting sensitivity and specificity estimates only as too simplistic. Users found the presentation of test accuracy for several values of prevalence initially confusing but modifying table layout and adding sample clinical scenarios for each prevalence reduced this confusion. Providing information about clinical consequences of testing result was viewed as not feasible for authors of systematic reviews.Conclusion
We present the current formats for tables presenting test accuracy following the GRADE approach. These tables can be developed using GRADEpro guidelines development tool (www.guidelinedevelopment.org or www.gradepro.org) and are being further developed into electronic interactive tables that will suit the needs of different end users. The formatting of these tables, and how they influence result interpretation and decision-making will be further evaluated in a randomized trial. 相似文献1000.
Nurettin Canakoglu Engin Berber Sukru Tonbak Mustafa Ertek Ibrahim Sozdutmaz Munir Aktas Ahmet Kalkan Aykut Ozdarendeli 《PLoS neglected tropical diseases》2015,9(3)
Crimean-Congo hemorrhagic fever (CCHF) is an acute tick-borne zoonotic disease. The disease has been reported in many countries of Africa, Asia, the Middle East, and in Eurasia. During the past decade, new foci of CCHF have emerged in the Balkan Peninsula, southwest Russia, the Middle East, western China, India, Africa, and Turkey. CCHF virus produces severe hemorrhagic manifestations in humans with fatality rates up to 30%. Vaccine development efforts have been significantly hampered by a lack of animal models and therefore, no protective vaccine has been achieved. Lately, IFN α/β receptor deficient (IFNAR−/−) mice have been established as a novel small animal model of CCHF virus infection. In the present study, we found that IFNAR−/− mice highly susceptible to CCHF virus Turkey-Kelkit06 strain. Immunization with the cell culture based vaccine elicited a significant level of protection against high dose challenge (1,000 PPFU) with a homologous CCHF virus in IFNAR−/− mice. 相似文献