The effect of follicular fluid hormones on oocyte recovery after ovarian stimulation: FSH level predicts oocyte recovery

Background  

Ovarian stimulation for assisted reproductive technology (ART) overcomes the physiologic process to develop a single dominant follicle. However, following stimulation, egg recovery rates are not 100%. The objective of this study is to determine if the follicular fluid hormonal environment is associated with oocyte recovery.     

Structural Maintenance of Chromosome (SMC) Proteins Link Microtubule Stability to Genome Integrity

Structural maintenance of chromosome (SMC) proteins are key organizers of chromosome architecture and are essential for genome integrity. They act by binding to chromatin and connecting distinct parts of chromosomes together. Interestingly, their potential role in providing connections between chromatin and the mitotic spindle has not been explored. Here, we show that yeast SMC proteins bind directly to microtubules and can provide a functional link between microtubules and DNA. We mapped the microtubule-binding region of Smc5 and generated a mutant with impaired microtubule binding activity. This mutant is viable in yeast but exhibited a cold-specific conditional lethality associated with mitotic arrest, aberrant spindle structures, and chromosome segregation defects. In an in vitro reconstitution assay, this Smc5 mutant also showed a compromised ability to protect microtubules from cold-induced depolymerization. Collectively, these findings demonstrate that SMC proteins can bind to and stabilize microtubules and that SMC-microtubule interactions are essential to establish a robust system to maintain genome integrity.     

Enzymatic activity of a soil profile in the Sudan Gezira

Summary In preliminary work in the enzymatic activity of a semi-arid Gezira soil microbes were counted and urease, dehydrogenase, and Catelase activities were measured in five soil horizons from 0 to 72 inches depth. Urease and dehydrogenase activities of unamended soil from all horizons appeared to be better indicators of biological status than catalase activity. Biological activity was marked in the top soil (0–2 inches) — the zone of grass root activity — but decreased gradually to reach a low level in deeper horizons.     

Diversity and phylogenetic relationships of <Emphasis Type="Italic">Glossina</Emphasis> populations in Nigeria and the Cameroonian border region

Background

Tsetse flies are vectors of trypanosomes, parasites that cause devastating disease in humans and livestock. In the course of vector control programmes it is necessary to know about the Glossina species present in the study area, the population dynamics and the genetic exchange between tsetse fly populations.

Results

To achieve an overview of the tsetse fly diversity in Nigeria and at the Nigeria-Cameroon border, tsetse flies were trapped and collected between February and March 2014 and December 2016. Species diversity was determined morphologically and by analysis of Cytochrome C Oxidase SU1 (COI) gene sequences. Internal transcribed spacer-1 (ITS-1) sequences were compared to analyse variations within populations. The most dominant species were G. m. submorsitans, G. tachinoides and G. p. palpalis. In Yankari Game Reserve and Kainji Lake National Park, G. submorsitans and G. tachinoides were most frequent, whereas in Old Oyo National Park and Ijah Gwari G. p. palpalis was the dominant species. Interestingly, four unidentified species were recorded during the survey, for which no information on COI or ITS-1 sequences exists. G. p. palpalis populations showed a segregation in two clusters along the Cameroon-Nigerian border.

Conclusions

The improved understanding of the tsetse populations in Nigeria will support decisions on the scale in which vector control is likely to be more effective. In order to understand in more detail how isolated these populations are, it is recommended that further studies on gene flow be carried out using other markers, including microsatellites.

     

Effects of hyperaemia on left ventricular longitudinal strain in patients with suspected coronary artery disease

Aims

Myocardial perfusion imaging during hyperaemic stress is commonly used to detect coronary artery disease. The aim of this study was to investigate the relationship between left ventricular global longitudinal strain (GLS), strain rate (GLSR), myocardial early (E’) and late diastolic velocities (A’) with adenosine stress first-pass perfusion cardiovascular magnetic resonance (CMR) imaging.

Methods and results

44 patients met the inclusion criteria and underwent CMR imaging. The CMR imaging protocol included: rest/stress horizontal long-axis (HLA) cine, rest/stress first-pass adenosine perfusion and late gadolinium enhancement imaging. Rest and stress HLA cine CMR images were analysed using feature-tracking software for the assessment of myocardial deformation. The presence of perfusion defects was scored on a binomial scale. In patients with hyperaemia-induced perfusion defects, rest global longitudinal strain GLS (?16.9 ± 3.7 vs. ?19.6 ± 3.4; p-value = 0.02), E’ (?86 ± 22 vs. ?109 ± 38; p-value = 0.02), GLSR (69 ± 31 vs. 93 ± 38; p-value = 0.01) and stress GLS (?16.5 ± 4 vs. ?21 ± 3.1; p < 0.001) were significantly reduced when compared with patients with no perfusion defects. Stress GLS was the strongest independent predictor of perfusion defects (odds ratio 1.43 95% confidence interval 1.14–1.78, p-value <0.001). A threshold of ?19.8% for stress GLS demonstrated 78% sensitivity and 73% specificity for the presence of hyperaemia-induced perfusion defects.

Conclusions

At peak myocardial hyperaemic stress, GLS is reduced in the presence of a perfusion defect in patients with suspected coronary artery disease. This reduction is most likely caused by reduced endocardial blood flow at maximal hyperaemia because of transmural redistribution of blood flow in the presence of significant coronary stenosis.

     

Somatic embryogenesis and plant regeneration in Anthurium scherzerianum

The effects of xylooligosaccharides isolated from the cell walls of Betula platyphylla var. japonica on cells and protoplasts of Pinus radiata were examined. The addition of a semi-purified mixture of xylooligosaccharides at a concentration of 5μg.ml⁻¹ promoted elongation of cultured cells, whereas the neutral fraction of this mixture had no effect; a similar effect was seen in the presence of conditioned medium. The unfractionated mixture of xylooligosaccharides was also found to enhance the viability of protoplasts prepared from cell cultures of Pinus radiata in a concentration dependent manner, highly similar to the effect provided by addition of medium conditioned by pine cells. Such effects are considered to be due to the addition of components that play a structural role in the cell wall of pines. It is inferred that the acidic components of the xylooligosaccharide mixture derived from t Betula are responsible for this effect in the distant pine species. It is speculated that acidic xylooligosaccharides operate either by replacing, or mimicking, the natural cell wall components required for growth and development of pine cultured cells. This revised version was published online in June 2006 with corrections to the Cover Date.     

Rice growth improvement and grains bio-fortification through lime and zinc application in zinc deficit tropical acid sulphate soils

A two years field study was conducted to explain the effect of Zn and lime application on morphological characteristics, rice yield and yield components, and more broadly, grains bio-fortification (Zn and protein content (CP), and amino acid profiles). The lime and Zn interaction increased grains and straw yield more than two times (6.64 ton ha^?1) compared to the control (3.20 ton ha^?1). The maximum increase in the Zn content of grain, white rice and bran was obtained about 30% in whole grain, 42% in bran and 56% in white rice. Furthermore, CP increased by about 8% in bran, 12.3% in whole grain, and 27% in white rice compared to control. Also, the Zn and lime application and their interaction were significantly increased the amino acids, especially essential parts.     

Sulfur and molybdenum fractionation in marine and riverine alluvium paddy soils

Intermittently submergence and drainage status of paddy fields can cause alterations in morphological and chemical characteristics of soils. We conducted a sequential fractionation study to provide an insight into solubility of Sulfur (S) and Molybdenum (Mo) in flooded alluvial paddy soils. The samples (0–15 and 15–30 cm) were taken from marine and riverine alluvial soils in Kedah and Kelantan areas, respectively, and were sequentially extracted with NaHCO₃, NaOH, HCl, and HClO₄–HNO₃. Total S in upper and lower layers of Kedah and Kelantan ranged between 273 and 1121 mg kg^?1, and 177 to 1509 mg kg^?1, respectively. In upper layers and subsoil of Kedah, average total Mo were 0.34 and 0.27 mg kg^?1, respectively. Average total Mo in Kelantan were 0.25 mg kg^?1 (surface layer) and 0.28 mg kg^?1 (subsoil). Cation exchange capacity (CEC) was positively correlated with plant available amounts of Mo in upper layers of Kedah area. Also, total and medium-term plant-available S was correlated with total carbon (C) at lower layers of Kelantan soil series. But in surface layers of Kelantan soil series, CEC was strongly correlated with total and medium-term plant-available S. Our results indicates that the influence of flooding conditions on soil S and Mo contents in paddy fields may cause long-term changes in S and Mo chemical reactivities.     

Novel inner membrane retention signals in Pseudomonas aeruginosa lipoproteins

The ultimate membrane localization and function of most of the 185 predicted Pseudomonas aeruginosa PAO1 lipoproteins remain unknown. We constructed a fluorescent lipoprotein, CSFP^OmlA-ChFP, by fusing the signal peptide and the first four amino acids of the P. aeruginosa outer membrane lipoprotein OmlA to the monomeric red fluorescent protein mCherry (ChFP). When cells were plasmolyzed with 0.5 M NaCl, the inner membrane separated from the outer membrane and formed plasmolysis bays. This permits the direct observation of fluorescence in either the outer or inner membrane. CSFP^OmlA-ChFP was shown to localize in the outer membrane by fluorescence microscopy and immunoblotting analysis of inner and outer membrane fractions. The site-directed substitution of the amino acids at positions +2, +3, and +4 in CSFP^OmlA-ChFP was performed to test the effects on lipoprotein localization of a series of amino acid sequences selected from a panel of predicted lipoproteins. We confirmed Asp⁺² and Lys⁺³ Ser⁺⁴ function as inner membrane retention signals and identified four novel inner membrane retention signals: CK⁺² V⁺³ E⁺⁴, CG⁺² G⁺³ G⁺⁴, CG⁺² D⁺³ D⁺⁴, and CQ⁺² G⁺³ S⁺⁴. These inner membrane retention signals are found in 5% of the 185 predicted P. aeruginosa lipoproteins. Full-length chimeras of predicted lipoproteins PA4370 and PA3262 fused to mCherry were shown to reside in the inner membrane and showed a nonuniform or patchy distribution in the membrane. The optical sectioning of cells producing PA4370^CGDD-ChFP and PA3262^CDSQ-ChFP by confocal microscopy improved the resolution and indicated a helix-like localization pattern in the inner membrane. The method described here permits the in situ visualization of lipoprotein localization and should work equally well for other membrane-associated proteins.