全文获取类型
收费全文 | 5998篇 |
免费 | 739篇 |
国内免费 | 12篇 |
专业分类
6749篇 |
出版年
2022年 | 39篇 |
2021年 | 86篇 |
2020年 | 43篇 |
2019年 | 51篇 |
2018年 | 63篇 |
2017年 | 56篇 |
2016年 | 97篇 |
2015年 | 174篇 |
2014年 | 188篇 |
2013年 | 234篇 |
2012年 | 282篇 |
2011年 | 301篇 |
2010年 | 160篇 |
2009年 | 150篇 |
2008年 | 282篇 |
2007年 | 263篇 |
2006年 | 254篇 |
2005年 | 245篇 |
2004年 | 218篇 |
2003年 | 200篇 |
2002年 | 188篇 |
2001年 | 207篇 |
2000年 | 214篇 |
1999年 | 164篇 |
1998年 | 80篇 |
1997年 | 79篇 |
1996年 | 78篇 |
1995年 | 62篇 |
1994年 | 78篇 |
1993年 | 79篇 |
1992年 | 149篇 |
1991年 | 136篇 |
1990年 | 129篇 |
1989年 | 115篇 |
1988年 | 106篇 |
1987年 | 121篇 |
1986年 | 99篇 |
1985年 | 101篇 |
1984年 | 96篇 |
1983年 | 75篇 |
1982年 | 63篇 |
1981年 | 67篇 |
1980年 | 51篇 |
1979年 | 69篇 |
1978年 | 56篇 |
1977年 | 44篇 |
1976年 | 47篇 |
1975年 | 39篇 |
1974年 | 45篇 |
1973年 | 58篇 |
排序方式: 共有6749条查询结果,搜索用时 15 毫秒
21.
Common mechanisms of promoter recognition by RNA polymerases II and III 总被引:26,自引:0,他引:26
22.
Glutathione- or sulfhydryl-dependent antioxidant factors that act to prevent lipid peroxidation have been reported in both microsomes and cytoplasm from rat liver. The cytoplasmic factor has been identified in several other tissues and species, but the distribution of the microsomal factor has not been reported. Chicken and mouse livers had much lower activities of the glutathione-dependent membrane-associated and cytoplasmic antioxidant factors than rat liver. Peroxidative damage to membranes has been hypothesized as a mechanism of tissue damage in muscular dystrophy. However, neither the chicken, mouse, nor rat had significant activities of the antioxidant factors in muscle. There was also no significant difference between normal and dystrophic chicken livers in the activity of the antioxidant factors associated with the microsomes or the cytoplasm, nor of the liver microsomal factor in normal and dystrophic mice. The results do not support an important role for the antioxidant factors in the pathogenesis of muscular dystrophy, and raise questions as to whether such factors are physiologically important in species other than rat or in tissues other than liver. 相似文献
23.
Cerebrospinal fluid (CSF) production results from active transport of Na+ from blood to CSF, which is followed by H2O and anions. Amiloride reduces Na+ movement in epithelial tissues. To ascertain if amiloride alters transport of Na+ in the choroid plexus, the drug was administered either i.p. to male Sprague-Dawley rats that were bilaterally nephrectomized to determine in vivo effects, or added to artificial CSF to incubate the choroid plexus in vitro. Choroid cell [Na+] was reduced after amiloride treatment both in vivo and in vitro. In addition, the rate of 22Na uptake into the CSF and choroid plexus (CP) was decreased after amiloride. Alterations in choroid cell [Na+] and 22Na penetration into CSF and CP occurred at relatively high doses of drug (1 mumol/ml, in vitro and 100 micrograms/g in vivo), but lower doses were less effective (0.1 mumol/ml in vitro and 10 micrograms/g in vivo). It is concluded that the effects of amiloride on Na+ distribution and transport in the CP are due to inhibition of basolateral Na+-H+ exchange. 相似文献
24.
25.
26.
27.
Purification and characterization of a bone metalloproteinase that degrades gelatin and types IV and V collagen 总被引:12,自引:0,他引:12
A third metalloendopeptidase activity, gelatinase, has been completely separated from the collagenase and proteoglycanase activities of rabbit bone culture medium. Although the proteinase could not be purified to homogeneity in large amounts, it was possible to obtain accurate molecular weight values and activity after electrophoresis on non-reduced SDS/polyacrylamide gels. The latent form had an Mr of 65 000 which could be activated with 4-aminophenylmercuric acetate, APMA, to a form of Mr 61 000; under reducing conditions the latent and active forms had Mr of 72 000 and 65 000, respectively. Trypsin was a very poor activator of the latent enzyme. Gelatinase degraded gelatins derived from the interstitial collagens and it also had low activity on native types IV and V collagen and on insoluble elastin. Gelatinase acted synergistically with collagenase in degrading insoluble interstitial collagen. The specific mammalian tissue inhibitor of metalloproteinases inhibited gelatinase by forming a stable inactive complex. Comparison of the properties of gelatinase with those of collagenase and proteoglycanase suggest that the three proteinases form a family which together are capable of degrading all the major macromolecules of connective tissue matrices. 相似文献
28.
We tested a number of inhibitory monovalent anions for their primary site of action on photosystem II(PSII) in chloroplasts. We find that the inhibitory effects of F−, HCO2−, NO2−, NO3−, and CH3CO2− are all reversed by addition of a high concentration of HCO3−. This class of anions competitively inhibits H14CO3− binding to PSII. All of those anions tested reduced H14CO3− binding more in the light than in the dark. We conclude that the primary inhibitory site of action of a number of monovalent anions is at the HCO3− binding site(s) on the PSII complex. The carbonic anhydrase inhibitor gold cyanide, and also azide, inhibit PSII but at a site other than the HCO3− binding site. We suggest that the unique ability of HCO3− to reverse the effects of inhibitory anions reflects its singular ability to act as a proton donor/acceptor at the anion binding site. A similar role has been proposed for non-substrate-bound HCO3− on carbonic anhydrase by Yeagle et al. (1975 Proc Natl Acad Sci USA 72: 454-458). 相似文献
29.
Jackson J. C.; Standaert T. A.; Truog W. E.; Murphy J. H.; Palmer S.; Chi E. Y.; Woodrum D. E.; Watchko J. F.; Hodson W. A. 《Journal of applied physiology》1985,59(6):1783-1789
Total lung capacity (TLC), inspiratory capacity, functional residual capacity, and deflation stability of prematurely delivered Macaca nemestrina primates were measured serially during development of, and recovery from, hyaline membrane disease (HMD) to relate changes in lung volumes to changes in deflation stability. Gestational age-matched primates that did not develop HMD served as controls. TLC, measured by N2 washout, fell at 2-12 h of age (P less than 0.0001) in animals with HMD and remained lower than controls for at least 48 h (P less than 0.005). However, deflation stability, defined as the fraction of TLC remaining upon deflation to 10 cm H2O, improved from 2 to 12 h of age (P less than 0.001). Postmortem studies confirm the measurements of TLC and deflation stability and provide evidence that interstitial thickening and obstruction of air spaces with debris may be partially responsible for the observed changes in TLC in primates that develop HMD. It has been assumed that TLC is reduced in HMD because of atelectasis from elevated alveolar surface tension, but the sequential measurements in these animals suggest that other mechanisms also contribute. 相似文献
30.
M L Heiman W A Murphy M V Nekola V A Lance D H Coy 《Biochemical and biophysical research communications》1984,124(1):217-222
The aim of this study was to investigate whether anterior pituitary responsiveness to human pancreatic growth hormone-releasing factor containing 29 amino acids (GRF-29) can be modulated by GRF-29 itself. Male rats were injected (sc) daily for 3 days with 50 ug of GRF-29, or were treated twice daily for 14 days with 5 ug of [D-Ala-2]-GRF-29 (a potent GRF agonist). Control animals were injected with saline. After the last injection, pituitaries were removed, dispersed, cultured for 96 h and then challenged with either GRF-29 or [D-Trp-6]-LHRH (a LHRH agonist). Cultured cells from analog-treated rats were more responsive to GRF-29 stimulation than were cells obtained from controls. In contrast, neither treatment altered the response to [D-Trp-6]-LHRH. These studies indicate that periodic administration of GRF analogs can increase hypophyseal GRF responsiveness. Such control may be an important component in the physiological regulation of GH secretion and has important implications for potential therapeutic uses of GRF analogs. 相似文献