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排序方式: 共有911条查询结果,搜索用时 9 毫秒
151.
Atina G Cote Jennifer Knapp Marta Verby Joseph C Mellor Yingzhou Wu Carles Pons Cassandra Wong Natascha van Lieshout Fan Yang Murat Tasan Guihong Tan Shan Yang Douglas M Fowler Robert Nussbaum Jesse D Bloom Marc Vidal David E Hill Patrick Aloy Frederick P Roth 《Molecular systems biology》2017,13(12)
Although we now routinely sequence human genomes, we can confidently identify only a fraction of the sequence variants that have a functional impact. Here, we developed a deep mutational scanning framework that produces exhaustive maps for human missense variants by combining random codon mutagenesis and multiplexed functional variation assays with computational imputation and refinement. We applied this framework to four proteins corresponding to six human genes: UBE2I (encoding SUMO E2 conjugase), SUMO1 (small ubiquitin‐like modifier), TPK1 (thiamin pyrophosphokinase), and CALM1/2/3 (three genes encoding the protein calmodulin). The resulting maps recapitulate known protein features and confidently identify pathogenic variation. Assays potentially amenable to deep mutational scanning are already available for 57% of human disease genes, suggesting that DMS could ultimately map functional variation for all human disease genes. 相似文献
152.
153.
Quraishi UM Abrouk M Murat F Pont C Foucrier S Desmaizieres G Confolent C Rivière N Charmet G Paux E Murigneux A Guerreiro L Lafarge S Le Gouis J Feuillet C Salse J 《The Plant journal : for cell and molecular biology》2011,65(5):745-756
Monitoring nitrogen use efficiency (NUE) in plants is becoming essential to maintain yield while reducing fertilizer usage. Optimized NUE application in major crops is essential for long-term sustainability of agriculture production. Here, we report the precise identification of 11 major chromosomal regions controlling NUE in wheat that co-localise with key developmental genes such as Ppd (photoperiod sensitivity), Vrn (vernalization requirement), Rht (reduced height) and can be considered as robust markers from a molecular breeding perspective. Physical mapping, sequencing, annotation and candidate gene validation of an NUE metaQTL on wheat chromosome 3B allowed us to propose that a glutamate synthase (GoGAT) gene that is conserved structurally and functionally at orthologous positions in rice, sorghum and maize genomes may contribute to NUE in wheat and other cereals. We propose an evolutionary model for the NUE locus in cereals from a common ancestral region, involving species specific shuffling events such as gene deletion, inversion, transposition and the invasion of repetitive elements. 相似文献
154.
155.
Ilkay Orhan Berrin Özçelik Sinem Aslan Murat Kartal Taner Karaoglu Bilge Şener Salih Terzioglu M. Iqbal Choudhary 《Phytochemistry Reviews》2007,6(1):189-196
Purpose of the present study was to evaluate antioxidant, antibacterial, antifungal, and antiviral activities of the petroleum
ether, chloroform, ethyl acetate and methanol extracts as well as the alkaloid fraction of Lycopodium clavatum L. (LC) from Lycopodiaceae growing in Turkey. Antioxidant activity of the LC extracts was evaluated by 1,1-diphenyl-2-picrylhydrazyl
(DPPH) radical-scavenging method at 0.2 mg/ml using microplate-reader assay. Antiviral assessment of LC extracts was evaluated
towards the DNA virus Herpes simplex (HSV) and the RNA virus Parainfluenza (PI-3) using Madin-Darby Bovine Kidney (MDBK) and Vero cell lines. Antibacterial and antifungal activities of the extracts
were tested against standard and isolated strains of the following bacteria; Escherichia coli, Pseudomonas aeruginosa, Proteus mirabilis, Acinobacter baumannii, Klebsiella pneumoniae, Staphylococcus aureus, Bacillus subtilis as well as the fungi; Candida albicans and C. parapsilosis. All of the extracts possessed noteworthy activity against ATCC strain of S. aureus (4 μg/ml), while the LC extracts showed reasonable antifungal effect. On the other hand, we found that only the chloroform
extract was active against HSV (16–8 μg/ml), while petroleum ether and alkaloid extracts inhibited potently PI-3 (16–4 μg/ml
and 32–4 μg/ml, respectively). However, all of the extracts had insignificant antiradical effect on DPPH. In addition, we
also analyzed the content of the alkaloid fraction of the plant by capillary gas chromatography-mass spectrometry (GC-MS)
and identified lycopodine as the major alkaloid. 相似文献
156.
Sleep and Biological Rhythms - 相似文献
157.
Allagui MS Nciri R Rouhaud MF Murat JC El Feki A Croute F Vincent C 《Neurochemical research》2009,34(3):453-462
SH-SY5Y cells, derived from a human neuroblastoma, were submitted to short- or long-term exposures to lithium carbonate concentrations
ranging from 0.5 to 8 mM. Short-term exposures (4 days) to concentrations higher than 6 mM were found to reduce cell growth
rate while exposure to 8 mM resulted in significant cell mortality. These ranges of concentrations induced an overexpression
of (1) the HSP27 stress protein, (2) a 108 kDa protein (P108) recognized by an anti-phospho-HSP27(Ser78) antibody, and probably
corresponding to a phosphorylated HSP27 tetramer, (3) a 105 kDa protein (P105), possible glycosylated or phosphorylated form
of the GRP94 stress protein and (4) a phosphorylated (inactivated) form of glycogen synthase kinase (GSK3α/β) SH-SY5Y cells,
when cultured in the presence of 0.5 mM lithium for 25 weeks, displayed interesting features as compared to controls: (1)
higher cell growth rate, (2) increased resistance toward the inhibitory effects of high lithium concentrations on cell proliferation,
(3) lower basal level of lipid peroxidation (TBARS) and improved tolerance to oxidative stress induced by high lithium concentrations,
(5) reduced expression of monomeric HSP27 versus an increase of corresponding tetrameric protein (P108) and (6) overexpression
of a 105 kDa protein (P105). In conclusion, our study suggests that chronic treatment (over several months) by therapeutic
relevant lithium concentrations could favour neurogenesis, decrease the vulnerability of neuronal cells to oxidative stress
and induce posttranslational changes of molecular chaperones. 相似文献
158.
Erdal Karaoz Ayça Aksoy Selda Ayhan Ayla Eker Sarıboyacı Figen Kaymaz Murat Kasap 《Histochemistry and cell biology》2009,132(5):533-546
Bone marrow-derived mesenchymal stem cells (BM-MSCs) can differentiate into many lineages. Although the growing interest in
BM-MSCs has led to a number of characterization studies, some important biochemical and immunohistochemical properties are
still lacking. In this study, morphological and immunophenotypic properties of BM-MSCs were examined in detail. Differentiation
potential and growth kinetics of adult rat BM-MSCs were also determined. Immunohistochemistry and RT-PCR results indicated
that BM-MSCs expressed myogenic (desmin, myogenin, myosin IIa, and α-SMA), neurogenic (γ-enolase, MAP2a,b, c-fos, nestin,
GFAP and beta III tubulin), and osteogenic (osteonectin, osteocalcin, osteopontin, Runx-2, BMP-2, BMP-4 and type I collagen)
markers without stimulation towards differentiation. These expression patterns indicated why these cells can easily differentiate
into multiple lineages both in vitro and in vivo. Ultrastructural characteristics of rBM-MSCs showed more developed and metabolically
active cells. 相似文献
159.
Erkan Yurtcu Akin Yilmaz Zubeyde Ozkurt Emine Kolukisa Murat Yilmaz Hatice Keles Mehmet Ali Ergun Ilhan Yetkin Adnan Menevse 《Biochemical genetics》2009,47(3-4):295-300
Obesity is a complex disease caused by both genetics and environmental factors. Melanocortin-4 receptor (MC4R) (MIM 155541) gene polymorphisms were reported to be the cause of monogenic obesity in humans. We studied three polymorphisms (Val50Met, Val103Ile, and Ser58Cys) and a mutation (Asn274Ser) of the MC4R gene in 203 obese patients and in 110 healthy subjects in the Turkish population. A high incidence of Val103Ile and Val50Met polymorphisms as well as the Asn274Ser mutation was found in the obese patients, whereas no significant correlation was found regarding the Ser58Cys polymorphism. We conclude that there is a concordance between the polymorphisms (Val103Ile, Val50Met, Ser58Cys) that were first studied in the Turkish population with obesity. 相似文献
160.