A simple and rapid method for the preparation of adenosine triphosphatase from submitochondrial particles.

An almost pure form of the bovine heart mitochondrial adenosine triphosphatase (ATPase) is released from the membrane by shaking submitochondrial particles with chloroform. Analyses on polyacrylamide gels and by electron microscopy, and also sensitivity to inhibitors, show that the chloroform-released enzyme is similar to other ATPase preparations from bovine heart mitochondria.     

Vitamin C amelioration of the adrenal stress response in broiler chickens being prepared for slaughter

1. At 32 hr prior to slaughter, broiler chickens were treated for 24 hr with either: 1200 ppm of L-ascorbic acid (AA; Vitamin C) in their drinking water or with no-AA supplementation (controls; simultaneously maintained on tap water). 2. Following the dietary treatments, birds were stressed by cooping/starvation for 8 hr before being hauled to an abattoir for processing. As indices of stress status, blood corticosterone (B) levels and heterophil/lymphocyte (H/L) ratios were determined at 32 and 8 hr prior to slaughter, and immediately before slaughter (0 hr). 3. The results indicated that AA supplementation reduces the stress response (i.e. elevated plasma B levels and H/L ratios) evident in broiler chickens being prepared for processing.     

Evidence that cutaneous antigen-presenting cells migrate to regional lymph nodes during contact sensitization

These studies address the hypothesis that Ag-bearing epidermal Langerhans cells migrate to the regional lymph node during contact sensitization and function as APC. Skin from C3H mice was grafted onto BALB/c nude mice, and 7 or 14 days later, the recipients were sensitized with FITC through the grafts. APC from lymph nodes draining the site of sensitization were capable of sensitizing C3H recipients to FITC. Because sensitization is MHC restricted, only cells reaching the lymph node from the grafted skin could have induced contact hypersensitivity in C3H mice. Examination of the FITC+ draining lymph node cells by immunofluorescence and immunoelectron microscopy demonstrated that all were Ia+, most were F4/80+, and some contained Birbeck granules. These studies demonstrate that Ia+, FITC+ cells from the skin, at least some of which are Langerhans cells, leave the skin after epicutaneous sensitization with FITC and participate in the initiation of the contact hypersensitivity response within the regional lymph node.