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81.
Reversal of X-inactivation in female mouse somatic cells hybridized with murine teratocarcinoma stem cells in vitro 总被引:14,自引:0,他引:14
A series of near-diploid embryonal carcinoma-like hybrid cells were obtained from polyethylene glycol mediated cell fusion between murine embryonal carcinoma cells (PSA-6TG1 or OTF9-63) having one X chromosome and thymocytes or bone marrow cells from female mice carrying Cattanach's or Searle's translocation. Prior to fusion with EC cells the somatic cells are presumed to contain only one active X chromosome. Following hybrid formation, the chronology of X chromosome replication and the expression of X-linked gene Pgk-1 indicated that all X chromosomes contributed by both parents were active in these hybrids. Experiments were performed to rule out the possibility that the hybrids were formed by fusion of EC cells with rare somatic cells in which both X chromosomes were active. Taken together the data indicate that within four days of fusion there is reactivation of the entire inactive X chromosome. 相似文献
82.
Koichiro Shimada Toyoko Yoshida Tetsuo Kuroishi Masahide Ishibashi 《Biochimica et Biophysica Acta (BBA) - Gene Structure and Expression》1983,740(2):169-178
A restriction map of chicken embryo lethal orphan (CELO) virus DNA was reported with ten restriction endonucleases (XbaI, XhoI, SalI, HindIII, EcoRI, BglI, KpnI, BamHI, PstI and SstI). CELO virus DNA was estimated by comparing CELO virus DNA fragments with marker DNA fragments to have a molecular weight of 29.3·106. 相似文献
83.
Kunio Yamato I-Yih Huang Helmut Muensch Akira Yoshida Heinz-Werner Goedde Dharam P. Agarwal 《Biochemical genetics》1983,21(1-2):135-145
The usualE 1 u and atypicalE 1 a human pseudocholinesterases (acylocholine acylhydrolase, EC 3.1.1.8) were purified to homogeneity. The active-site serine residue was conjugated with diisopropyl fluorophosphate and digested with trypsin. The tryptic peptide containing the active site was isolated by gel filtration followed by two-dimensional paper chromatography and electrophoresis. The amino acid sequence of the active site peptide obtained from the usualE 1 u enzyme was found to be Gly-Glu-Ser-Ala-Gly-Ala-Ser-Ala-Val-Ser-Leu. A remarkable structural homology exists between the human and the horse enzymes in their active sites. From the difference in electrophoretic mobility of the active-site peptides obtained from the usual and atypical enzymes, the probable structure of the atypical human enzyme was deduced as Gly-His-Ser-Ala-Gly-Ala-Ser-Ala-Val-Ser-Leu. 相似文献
84.
85.
The nucleotide sequency of tRNA Gly from yeast 总被引:6,自引:0,他引:6
M Yoshida 《Biochemical and biophysical research communications》1973,50(3):779-784
Analysis of the factor for proximity and orientation is given for cyclopentadiene dimerization and for the general case of more complex biological molecules. Using either a probability or a statistical mechanical approach, the same ratios are obtained. The proximity effect factor is found to be about per reacting pair of molecules which show no net attraction or repulsion. The use of translational entropy without inclusion of a rotational entropy term gives a misleading value for this proximity factor. The calculations support the argument that orientation factors can play a large role in the catalytic power of enzymes. 相似文献
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A novel composition of phosvitins from salmon and trout roe 总被引:1,自引:0,他引:1
90.