Competitive strategies between Anastrepha fraterculus and Anastrepha obliqua (Diptera: Tephritidae) regulating the use of host fruits

Interspecific competition between individuals of different species can result in reductions in their fecundity, growth or survival, reflecting differential exploitation of resources that become intensified due to spatial co-occurrence, ecological similarity and increased population densities. As two species cannot occupy the same niche, coexistence is only possible if the available resources are used in non-overlapping manners such as niche partitioning or the use of refuges. Among agricultural insect pests, such as fruit flies of the family Tephritidae, competitive interactions can result in competitive displacement, host changes, or the expansion or restriction of the numbers of hosts utilized that can have negative consequences for human agricultural activities. We evaluated the competitive interactions between two fruit fly species of the genus Anastrepha, Anastrepha obliqua (Macquart, 1835) and Anastrepha fraterculus (Wiedmann, 1830), on their respective preferred hosts (mangoes and guava). Experiments of larval competition and competition for ovipositioning sites by adult females were performed to compare the parameters of larval development time, numbers of pupae and emerged adults and numbers of ovipositions in the presence or absence of interspecific competition. We observed that the interactions between those species were asymmetrical and hierarchical, and our results suggest a competitive displacement of A. fraterculus by A. obliqua when those two species are present on the same fruit, whether mangoes or guavas.     

Using untapped telemetry data to explore the winter biology of freshwater fish

Winter is a challenging period for aquatic research—weather is uncomfortable, ice is hazardous, equipment fails, and daylength is short. Consequently, until recently relatively little research on freshwater fishes has included winter. Telemetry methods for tracking fish and observing movement behavior are an obvious solution to working in harsh conditions because much of the data can be collected remotely, and passive methods collect data year-round without winter maintenance. Yet, many telemetry studies do not collect data during winter or, if they do, only report data from the ice-free seasons while the remaining data are unused. Here, we briefly summarize the advantages and limitations of using telemetry methods in winter, including acoustic and radio telemetry and passive integrated transponder technology, then review the range of questions related to fish ecology, behavior, bioenergetics, and habitat use that can be addressed in winter using telemetry. Our goals are to highlight the untapped potential of winter fish biology and to motivate scientists to revisit their four-season telemetry data and incorporate objectives specific to winter biology in future study plans.

     

Resveratrol Prevents Stress-Related Dysfunction of Mitochondria

This study was conducted to investigate the antistress potential of resveratrol, a natural polyphenol, in models that reproduce the conditions of acute hypobaric hypoxia and acute alcohol intoxication. Acute alcohol intoxication and acute hypobaric hypoxia induced an increase in the intensity of lipid peroxidation in the membranes of liver mitochondria from mice. Activation of lipid peroxidation was accompanied by swelling and variations in the levels of fatty acids with C₁₈ and C_20–22 in the composition of the total lipid fraction of mitochondrial membranes. The index of the unsaturation of fatty acids with C₁₈ was decreased by 7.5% (from 1.69 ± 0.01 to 1.52 ± 0.01). Furthermore, the (20:3ω6+20:5ω3)/22:6ω3 index decreased from 0.23 ± 0.02 to 0.13 ± 0.01 for fatty acids under acute hypobaric hypoxia conditions, suggesting a decrease in eicosanoid metabolism. The administration of 2 × 10^–5 mol/kg of resveratrol in animals for 5 days prevented changes in fatty acid composition, inhibiting activation of lipid peroxidation and swelling of mitochondria, thereby affecting physiological parameters. Thus, the adaptogenic properties of resveratrol may be ascribed to the prevention of lipid peroxidation in mitochondrial membranes, which probably affects the functional state of these organelles, contributing to the maintenance of cellular energy metabolism under stress conditions.

     

MyD88 oligomer size functions as a physical threshold to trigger IL1R Myddosome signaling

A recurring feature of innate immune receptor signaling is the self-assembly of signaling proteins into oligomeric complexes. The Myddosome is an oligomeric complex that is required to transmit inflammatory signals from TLR/IL1Rs and consists of MyD88 and IRAK family kinases. However, the molecular basis for how Myddosome proteins self-assemble and regulate intracellular signaling remains poorly understood. Here, we developed a novel assay to analyze the spatiotemporal dynamics of IL1R and Myddosome signaling in live cells. We found that MyD88 oligomerization is inducible and initially reversible. Moreover, the formation of larger, stable oligomers consisting of more than four MyD88s triggers the sequential recruitment of IRAK4 and IRAK1. Notably, genetic knockout of IRAK4 enhanced MyD88 oligomerization, indicating that IRAK4 controls MyD88 oligomer size and growth. MyD88 oligomer size thus functions as a physical threshold to trigger downstream signaling. These results provide a mechanistic basis for how protein oligomerization might function in cell signaling pathways.