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71.
Bahrami G Mohammadi B Kiani A 《Journal of chromatography. B, Analytical technologies in the biomedical and life sciences》2008,872(1-2):38-42
A new straightforward method based on cloud-point extraction (CPE) has been developed, optimized and validated for the determination of venlafaxine in human plasma by reversed-phase high-performance liquid chromatography with fluorescence detection. The non-ionic surfactant Triton X-114 (polyethylene glycol tert-octylphenyl ether) was chosen as the extract solvent. Separation was obtained using a reversed-phase Diamonsil column (C(18), 250mmx4.6mm I.D., 5mum) and a mobile phase composed of acetonitrile-phosphate buffer solution (pH 3.0)-triethylamine (33.5:66.5:0.4). Fluorescence detection was used (lambda(ex) 276nm, lambda(em) 598nm). Maprotiline was used as the internal standard. Under the optimum conditions, the linear range of venlafaxine in human plasma was 10-800ngmL(-1) (r(2)=0.9995). The limit of detection (LOD) was less than 2ngmL(-1) (S/N=3) and the limit of quantification (LOQ) was less than 10ngmL(-1) (S/N=10). The method was successfully applied for the evaluation of pharmacokinetic profiles of venlafaxine capsules in nine healthy volunteers. 相似文献
72.
Medici D Razzaque MS Deluca S Rector TL Hou B Kang K Goetz R Mohammadi M Kuro-O M Olsen BR Lanske B 《The Journal of cell biology》2008,182(3):459-465
Fibroblast growth factor 23 (FGF-23) and Klotho are secretory proteins that regulate mineral-ion metabolism. Fgf-23(-/-) or Klotho(-/-) knockout mice exhibit several pathophysiological processes consistent with premature aging including severe atrophy of tissues. We show that the signal transduction pathways initiated by FGF-23-Klotho prevent tissue atrophy by stimulating proliferation and preventing apoptosis caused by excessive systemic vitamin D. Because serum levels of active vitamin D are greatly increased upon genetic ablation of Fgf-23 or Klotho, we find that these molecules have a dual role in suppression of apoptotic actions of vitamin D through both negative regulation of 1alpha-hydroxylase expression and phosphoinositide-3 kinase-dependent inhibition of caspase activity. These data provide new insights into the physiological roles of FGF-23 and Klotho. 相似文献
73.
Identification of Botrytis spp. on Plants Grown in Iran 总被引:1,自引:0,他引:1
S. Mirzaei E. Mohammadi Goltapeh M. Shams-Bakhsh N. Safaie 《Journal of Phytopathology》2008,156(1):21-28
A total of 363 isolates were collected from all over Iran. They were isolated from apple, arum lily, briar rose, bride wort, broad bean, camellia, canola, carnation, cucumber, egg plant, feijoa, geranium, gerbera, gladiolus, grape, guilder rose, hibiscus, iris, kiwifruit, oleander, onion, orange, pear, pomegranate, primrose, quince, redbud, robinia, rose, rubber plant, sow thiste, spathe flower, strawberry, tomato, violet, wall flower and wheat. To identify the species, morphological characters such as conidiophore length, conidial and sclerotial dimensions were measured. According to morphological and cultural characters, eight Botrytis species were identified: B. aclada sensu lato, B. cinerea, B. fabae, B. convoluta, B. gladiolorum, B. paeoniae, B. pelargonii and B. porri. As far as we are aware, this is the first report of the last five species from Iran. These species were examined by polymerase chain reaction (PCR) using necrosis and ethylene‐inducing protein (NEP2) and C729 primers. A 835 bp band was amplified in B. cinerea, B. fabae and B. pelargonii, using NEP2, but not in others. However, C729 primers amplified a 700 bp band in B. cinerea and B. pelargonii and a 600 bp in B. fabae. 相似文献
74.
A.R. Eivazi M.R. Naghavi M. Hajheidari S.M. Pirseyedi M.R. Ghaffari S.A. Mohammadi I. Majidi G.H. Salekdeh & M. Mardi 《The Annals of applied biology》2008,152(1):81-91
The genetic diversity among 10 Iranian bread wheat (Triticum aestivum) genotypes was analysed using 12 quality traits, 320 amplified fragment length polymorphisms (AFLP) polymorphic fragments, 491 simple sequence repeats (SSR) alleles and 294 proteome markers. The results revealed that the genotypes differed for quality traits, AFLP, SSR and proteome markers. The average genetic diversity based on quality traits (0.684 with a range of 0.266–0.997) was higher than AFLP (0.502 with a range of 0.328–0.717), SSR (0.503 with a range of 0.409–0.595) and proteome (0.464 with a range of 0.264–0.870) markers. Although there were apparent similarities between the groupings of particular genotypes, the overall correspondence between the distance matrices appeared to be rather low. In this study, the cluster analysis based on AFLP data showed the closest agreement with genotypes’ regions of origin or pedigree information. In addition to the genetic diversity assessment, specific proteins with known function were detected uniquely for the studied genotypes. Our results suggest that the classification based on quality traits and genotypic markers of these wheat genotypes will be useful for wheat breeders to plan crosses for positive traits. 相似文献
75.
Payam Pour Mohammadi Ahmad Moieni Asa Ebrahimi Farzad Javidfar 《Plant Cell, Tissue and Organ Culture》2012,108(2):251-256
An efficient method for producing doubled haploid plants of oilseed rape (Brassica
napus L.) was established using in vitro colchicine treatment of haploid embryos. Haploid embryos in the cotyledonary stage were
treated with one of four colchicine concentrations (125, 250, 500 and 1,000 mg/L); for one of three treatment durations (12,
24 and 36 h) at one of the two temperatures (8 and 25°C) and were compared to control embryos (without colchicine treatment).
The number of chromosomes, seed recovery, size and density of leaf stomata, and pollen grain size from regenerated plants
were determined. No doubled haploid plants were regenerated from control embryos; however, the doubled haploid plants were
regenerated from colchicine-treated embryos. A high doubling efficiency, 64.29 and 66.66% of regenerated plants, was obtained
from 250 mg/L colchicine treatment for 24 h and 500 mg/L colchicine treatment for 36 h, respectively, at 8°C. Following 500 mg/L
colchicine treatment for 36 h, a few plants regenerated (9 plants). At the higher colchicine concentration (1,000 mg/L), no
plant regenerated. These results indicate that the colchicine treatment of embryos derived from microspores can induce efficient
chromosome doubling for the production of doubled haploid lines of oilseed rape. 相似文献
76.
Mousavi Shokouh Najafpour Ghasem D. Mohammadi Maedeh Seifi Mohammad Hasan 《Bioprocess and biosystems engineering》2018,41(4):519-530
Bioprocess and Biosystems Engineering - Cultivation of microalgae in wastewater is a promising and cost-effective approach for both CO2 biofixation and wastewater remediation. In this study, a new... 相似文献
77.
Neil A. Koorbanally Neil R. Crouch Avinash Harilal Bavani Pillay Dulcie A. Mulholland 《Biochemical Systematics and Ecology》2006
We report on the first phytochemical investigation of a member of the African genus Resnova (Hyacinthoideae: Hyacinthaceae). From the dichloromethane extract of the bulbs of both Resnova humifusa and Eucomis montana (Hyacinthoideae: Hyacinthaceae) a novel 3-benzyl-4-chromanone homoisoflavonoid, 5,6-dimethoxy-7-hydroxy-3-(4′-hydroxybenzyl)-4-chromanone, was isolated. A further 11 known homoisoflavonoids were also identified, the 12 in total presenting a clear biosynthetic sequence. Eight of the 12 compounds found were common to both species. 相似文献
78.
79.
Biomechanics and Modeling in Mechanobiology - This paper is focused on specification of conditions at failure in bovine cortical bone. Both experimental and analytical studies are conducted. The... 相似文献
80.