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131.
Zhang Hongling Ran Chao Teame Tsegay Ding Qianwen Hoseinifar Seyed Hossein Xie Mingxu Zhang Zhen Yang Yalin Olsen Rolf Erik Gatlin Delbert M. Ringø Einar Duan Ming Zhou Zhigang 《Reviews in Fish Biology and Fisheries》2020,30(4):569-586
Reviews in Fish Biology and Fisheries - The intestinal mucosal barrier plays a critical role in the maintenance of host health. In farmed teleost fish, the intestinal epithelium is challenged by a... 相似文献
132.
以甘肃省的民勤绿洲为例,选择土壤水分最为亏缺的7月,对白刺(Nitraria tangutorum)沙包不同迎风面,以及绿洲和荒漠交错地带的土壤水分进行了取样分析。结果表明,对于白刺沙包而言,其上层和深层土壤水分是迎风坡大于背风坡,中间层次(40~80 cm)则刚好相反。在绿洲与荒漠的交错带上,灌丛沙包与沙丘间低地水分变化有很大的不同,总体而言,丘间地水分含量大于灌丛沙包,但在距离绿洲406 m处,二者均出现一个土壤水分的最低值。在丘间地表层,水分变化是距离绿洲愈近,土壤含水量愈高;而深层土壤水分的变化趋 相似文献
133.
Yating Wang Jun Wang Ding Bai Jinlin Song Rui Ye Zhihe Zhao Lei Lei Jin Hao Chunmiao Jiang Shanbao Fang Shu An Qian Cheng Juan Li 《Journal of cellular physiology》2013,228(9):1935-1942
The presence of an appropriate number of viable cells is prerequisite for successive differentiation during chondrogenesis. Chondrogenic differentiation has been reported to be influenced by mechanical stimuli. This research aimed to study the effects of cyclic compressive stress on cell viability of rat bone marrow‐derived MSCs (BMSCs) during chondrogenesis as well as its underlying mechanisms. The results showed that dynamic compression increased cell quantity and viability remarkably in the early stage of chondrogenesis, during which the expression of Ihh, Cyclin D1, CDK4, and Col2α1 were enhanced significantly. Possible signal pathways implicated in the process were explored in our study. MEK/ERK and p38 MAPK were not found to function in this process while BMP signaling seemed to play an important role in the mechanotransduction during chondrogenic proliferation. In conclusion, dynamic compressive stress could enhance cell viability during chondrogenesis, which might be achieved by activating BMP signaling. J. Cell. Physiol. 228: 1935–1942, 2013. © 2013 Wiley Periodicals, Inc. 相似文献
134.
重组大肠杆菌表达铜绿假单胞菌溶血性磷脂酶C 总被引:1,自引:0,他引:1
[目的]构建产溶血性磷脂酶C (Hemolytic Phospholipase C,PLCH)的重组大肠杆菌(Escherich coli菌株,并初步优化其发酵条件.[方法]首先利用卵黄硼砂平板分离法筛选到一株产磷脂酶C(Phospholipase C,PLC)活性较高的菌株,命名为铜绿假单胞菌(Pseudomonas aeruginosa)41;进一步以P.aeruginosa 41基因组DNA为模板设计引物,PCR扩增获得溶血性磷脂酶C(PLCH)基因,构建重组大肠杆菌表达质粒并转化大肠杆菌E.coli BL21 (DE3);筛选转化子并检测PLC活性和溶血能力,并初步优化其发酵条件.[结果]成功构建了重组大肠杆菌E.coli BL21(DE3) /pET28a-plcH;在硼砂卵黄平板上对重组菌进行PLC活性测定,显示重组菌有明显的磷脂酶C活性;在哥伦比亚血琼脂平板上对重组菌进行溶血性试验,表明PLCH具有较强的溶血活性;初步优化摇瓶发酵条件为:5%转接量,37℃、200 r/min下培养4h添加IPTG至终浓度为0.9 mmol/L,转为25℃、150 r/min诱导培养14 h;优化后重组菌的酶活可达到722.89±0.47 U/mL.[结论]本文成功构建了一株产溶血性磷脂酶C活性较高的重组大肠杆菌菌株,并通过优化发酵条件使其酶活达到了722.89±0.47 U/mL,本实验在国内首次实现了铜绿假单胞菌来源的溶血性磷脂酶C基因在大肠杆菌的胞内表达,该研究为研究磷脂酶C产业化奠定了一定的基础. 相似文献
135.
Bai Y Xue Y Gao H Wang L Ding T Bai W Fan A Zhang J An Q Xu Z 《Protein expression and purification》2008,59(2):189-196
The completion of Mycobacterium tuberculosis genome sequence has opened a new way for the identification and characterization of bacterial antigens, such as ESAT-6, CFP10, MPT64, and Ag85 complex, which are helpful for tuberculosis control. In this work, genes of ESAT-6 and MPT64 were fused and expressed in Escherichia coli in form of inclusion bodies with a histidine tag. The expressed fusion protein was purified by nitrilotriacetic acid (Ni-NTA) affinity chromatography under denaturing conditions, and the yield was 18mg/L of culture. In mice, the purified ESAT-6-MPT64 fusion protein elicited stronger humoral response, greater splenic lymphocyte stimulated index, and higher levels of IFN-gamma and IL-12 production than that of the single MPT64 inoculation group, and rendered modest protection on the experimental tuberculosis mouse models. In short, the ESAT-6-MPT64 fusion protein might be a potential candidate vaccine for tuberculosis. 相似文献
136.
A hidden aggregation‐prone structure in the heart of hypoxia inducible factor prolyl hydroxylase 下载免费PDF全文
Hamid Hadi‐Alijanvand Elizabeth A. Proctor Feng Ding Nikolay V. Dokholyan Ali A. Moosavi‐Movahedi 《Proteins》2016,84(5):611-623
Prolyl hydroxylase domain‐containing protein 2 (PHD2), as one of the most important regulators of angiogenesis and metastasis of cancer cells, is a promising target for cancer therapy drug design. Progressive studies imply that abnormality in PHD2 function may be due to misfolding. Therefore, study of the PHD2 unfolding pathway paves the way for a better understanding of the influence of PHD2 mutations and cancer cell metabolites on the protein folding pathway. We study the unfolding of the PHD2 catalytic domain using differential scanning calorimetry (DSC), fluorescence spectroscopy, and discrete molecular dynamics simulations (DMD). Using computational and experimental techniques, we find that PHD2 undergoes four transitions along the thermal unfolding pathway. To illustrate PHD2 unfolding events in atomic detail, we utilize DMD simulations. Analysis of computational results indicates an intermediate species in the PHD2 unfolding pathway that may enhance aggregation propensity, explaining mutation‐independent PHD2 malfunction. Proteins 2016; 84:611–623. © 2016 Wiley Periodicals, Inc. 相似文献
137.
为了筛选冬青属(Ilex)植物优良防火品种,了解该属植物叶片燃烧表现特征,优化园林树种防火性能的评价方法,该文以克恩氏冬青(Ilex×koehneana)、史蒂芬冬青(Ilex ‘Nellie R. Stevens’)、大别山冬青(I. dabieshanensis)、博福德冬青(I. cornuta ‘Burfordii’)、双核冬青(I. dipyrena)为研究对象,以海桐(Pittosporum tobira)和石楠(Photinia serrulata)作为参照,采用因子分析法对这七种植物鲜叶和干叶的防火性能分别进行综合评价。结果表明:(1)不同冬青品种鲜叶火灾性能指数存在显著性差异,其余指标均呈极显著性差异。(2)经过因子分析进行评价得出鲜叶抗火性从强到弱依次为博福德冬青史蒂芬冬青大别山冬青石楠海桐双核冬青克恩氏冬青;干叶抗火性从强到弱依次为史蒂芬冬青大别山冬青博福德冬青海桐双核冬青克恩氏冬青石楠。(3)综合鲜叶及干叶的评分结果,得出博福德冬青、史蒂芬冬青、大别山冬青得分较高,防火性能优于石楠和海桐,防火性能表现更突出。 相似文献
138.
139.
Jieyao Cheng Jinlin Hou Huiguo Ding Guofeng Chen Qing Xie Yuming Wang Minde Zeng Xiaojuan Ou Hong Ma Jidong Jia 《PloS one》2015,10(12)
Background and Aims
Noninvasive models have been developed for fibrosis assessment in patients with chronic hepatitis B. However, the sensitivity, specificity and diagnostic accuracy in evaluating liver fibrosis of these methods have not been validated and compared in the same group of patients. The aim of this study was to verify the diagnostic performance and reproducibility of ten reported noninvasive models in a large cohort of Asian CHB patients.Methods
The diagnostic performance of ten noninvasive models (HALF index, FibroScan, S index, Zeng model, Youyi model, Hui model, APAG, APRI, FIB-4 and FibroTest) was assessed against the liver histology by ROC curve analysis in CHB patients. The reproducibility of the ten models were evaluated by recalculating the diagnostic values at the given cut-off values defined by the original studies.Results
Six models (HALF index, FibroScan, Zeng model, Youyi model, S index and FibroTest) had AUROCs higher than 0.70 in predicting any fibrosis stage and 2 of them had best diagnostic performance with AUROCs to predict F≥2, F≥3 and F4 being 0.83, 0.89 and 0.89 for HALF index, 0.82, 0.87 and 0.87 for FibroScan, respectively. Four models (HALF index, FibroScan, Zeng model and Youyi model) showed good diagnostic values at given cut-offs.Conclusions
HALF index, FibroScan, Zeng model, Youyi model, S index and FibroTest show a good diagnostic performance and all of them, except S index and FibroTest, have good reproducibility for evaluating liver fibrosis in CHB patients.Registration Number
ChiCTR-DCS-07000039. 相似文献140.
Ning Ding Shu Wang Yan Xiao Shuai Huang Lin Chen Jiang Xie Xian-Li Zhou 《化学与生物多样性》2023,20(5):e202300058
Four new aconitine-type C19-diterpenoid alkaloids, were isolated from the roots of Aconitum nagarum Stapf which were named as nagarutines A–D ( 1–4 ), together with eleven known compounds ( 5–15 ). The structures of the compounds were identified by IR, HR-ESI-MS, 1D and 2D NMR spectra. All compounds were tested for the inhibitory effect on LPS induced NO production in RAW 264.7 macrophages, compound 7 showed moderate anti-inflammatory activity effect and Inhibition rate is about 44.50%. 相似文献