全文获取类型
收费全文 | 143篇 |
免费 | 10篇 |
出版年
2022年 | 2篇 |
2021年 | 3篇 |
2020年 | 8篇 |
2019年 | 9篇 |
2018年 | 5篇 |
2017年 | 3篇 |
2016年 | 5篇 |
2015年 | 5篇 |
2014年 | 4篇 |
2013年 | 6篇 |
2012年 | 12篇 |
2011年 | 17篇 |
2010年 | 8篇 |
2009年 | 7篇 |
2008年 | 7篇 |
2007年 | 7篇 |
2006年 | 4篇 |
2005年 | 2篇 |
2004年 | 2篇 |
2003年 | 5篇 |
2002年 | 2篇 |
2001年 | 2篇 |
2000年 | 3篇 |
1999年 | 1篇 |
1998年 | 2篇 |
1997年 | 1篇 |
1995年 | 2篇 |
1993年 | 1篇 |
1991年 | 3篇 |
1990年 | 1篇 |
1982年 | 1篇 |
1978年 | 1篇 |
1977年 | 1篇 |
1976年 | 1篇 |
1974年 | 2篇 |
1972年 | 1篇 |
1970年 | 2篇 |
1969年 | 2篇 |
1966年 | 1篇 |
1964年 | 2篇 |
排序方式: 共有153条查询结果,搜索用时 31 毫秒
21.
Markus Grabenbauer Kurt Sätzler Eveline Baumgart H. Dariush Fahimi 《Cell biochemistry and biophysics》2000,32(1-3):37-49
Peroxisomes in the human hepatoblastoma cell line, HepG2, exhibit distinct alterations of shape, size, and distribution, dependent
on culture conditions (cell density, duration in culture, and presence of specific growth factors). Although many cells with
elongated tubular peroxisomes are present in thinly seeded cultures, spherical particles forming large focal clusters are
found in confluent cultures. The authors have analyzed the ultrastructure and the spatial relationship of peroxisomes of HepG2
cells at different stages of differentiation, using three-dimensional (3D)-reconstruction of ultrathin serial sections, and
electronic image processing. Cells were prepared for immunofluorescence using different antibodies against peroxisomal matrix
and membrane proteins, as well as for electron microscopy after the alkaline 3,3′-diaminobenzidine staining for catalase.
The results indicate that the tubular peroxisomes, which can reach a length of several microns, are consistently isolated,
and never form an interconnected peroxisomal reticulum. At the time of disappearance of tubular peroxisomes, rows of spherical
peroxisomes, arranged like beads on a string, are observed, suggesting fission of tubular ones. In differentiated confluent
cultures, clusters of several peroxisomes are seen, which, by immunofluorescence, appear as large aggregates, but after 3D
reconstruction consist of single spherical and angular peroxisomes without interconnections. The majority of such mature spherical
peroxisomes (but not the tubular ones) exhibit tail-like, small tubular and vesicular attachments to their surface, suggesting
a close functional interaction with neighboring organelles, particularly the endoplasmic reticulum, which is often observed
in close vicinity of such peroxisomes. 相似文献
22.
The peroxisome: an update on mysteries 总被引:1,自引:0,他引:1
Peroxisomes contribute to several crucial metabolic processes such as β-oxidation of fatty acids, biosynthesis of ether phospholipids and metabolism of reactive oxygen species, which render them indispensable to human health and development. Peroxisomes are highly dynamic organelles that rapidly assemble, multiply and degrade in response to metabolic needs. In recent years, the interest in peroxisomes and their physiological functions has significantly increased. This review intends to highlight recent discoveries and trends in peroxisome research, and represents an update as well as a continuation of a former review article. Novel exciting findings on the biological functions, biogenesis, formation and degradation of peroxisomes, on peroxisomal dynamics and division, as well as on the interaction and cross-talk of peroxisomes with other subcellular compartments are addressed. Furthermore, recent findings on the role of peroxisomes in the brain are discussed. 相似文献
23.
24.
Sanam Sadreddini Behzad Baradaran Ali Aghebati-Maleki Sevil Sadreddini Dariush Shanehbandi Ali Fotouhi Leili Aghebati-Maleki 《Journal of cellular physiology》2019,234(6):8541-8549
Among the main promising systems to triggering therapeutic antitumor immunity is the blockade of immune checkpoints. Immune checkpoint pathways regulate the control and eradication of infections, malignancies, and resistance against a host of autoantigens. Initiation point of the immune response is T cells, which have a critical role in this pathway. As several immune checkpoints are initiated by ligand–receptor interactions, they can be freely blocked by antibodies or modulated by recombinant forms of ligands or receptors. Antibodies against cytotoxic T-lymphocyte-associated antigen 4 (CTLA-4) were the first immunotherapeutics that achieved the US Food and Drug Administration approval. Preliminary clinical results with the blockers of additional immune checkpoint proteins, such as programmed cell death protein 1 (PD-1) indicate extensive and different chances to boost antitumor immunity with the objective of conferring permanent clinical effects. This study provides an overview of the immune checkpoint pathways, including CTLA-4, PD-1, lymphocyte activation gene 3, T-cell immunoglobulin and mucin domain 3, B7-H3, and diacylglycerol kinase α and implications of their inhibition in the cancer therapy. 相似文献
25.
26.
Dariush Shokri Mohammad Rabbani Khorasgani Milad Mohkam Seyed Masih Fatemi Younes Ghasemi Asghar Taheri-Kafrani 《Probiotics and antimicrobial proteins》2018,10(1):34-42
The emergence of antibiotic-resistant and food-spoilage microorganisms has renewed efforts to identify safe and natural alternative agents of antibiotics such as probiotics. The aim of this study was the isolation of lactobacilli as potential probiotics from local dairy products with broad antibacterial and anti-biofilm activities against antibiotic-resistant strains of Pseudomonas aeruginosa and determination of their inhibition mechanism. Antibiotic susceptibility and classification of acquired resistance profiles of 80 P. aeruginosa strains were determined based on Centers for Disease Control and Prevention (CDC) new definition as multidrug-resistant (MDR), extensively drug-resistant (XDR), and pan-drug-resistant (PDR) followed by antibacterial assessment of lactobacilli against them by different methods. Among the 80 P. aeruginosa strains, 1 (1.3%), 50 (62.5%), and 78 (97.5%) were PDR, XDR, and MDR, respectively, and effective antibiotics against them were fosfomycin and polymyxins. Among 57 isolated lactobacillus strains, two strains which were identified as Lactobacillus fermentum using biochemical and 16S rDNA methods showed broad inhibition/killing and anti-biofilm effects against all P. aeruginosa strains. They formed strong biofilms and had bile salts and low pH tolerance. Although investigation of inhibition mechanism of these strains showed no bacteriocin production, results obtained by high-performance liquid chromatography (HPLC) analysis indicated that their inhibitory effect was the result of production of three main organic acids including lactic acid, acetic acid, and formic acid. Considering the broad activity of these two L. fermentum strains, they can potentially be used in bio-control of drug-resistant strains of P. aeruginosa. 相似文献
27.
Summary The immunofluorescence technique has become an important tool for the investigation of peroxisomes in cell culture. We have used this method for the study of peroxisomes in the human hepatoblastoma cell line HepG2. A marked heterogeneity of peroxisomal forms was detected. Besides spherical (about 100 nm) and rod-shaped structures (about 300 nm) many elongated, undulating tubular forms (up to 5 m) were found. Further observations indicate that the appearance of the peroxisomal forms in immunofluorescence is dependent on the fixation procedure used. Whereas the fixation with methanol-acetone (–20°C) or ethanol results in a punctate pattern with spherical particles, the use of formaldehyde/Triton X-100 fixation shows well-preserved tubules and rods. These observations may be of special importance for studies on the biogenesis of peroxisomes. 相似文献
28.
29.
30.
Russo R Fox E Cinel C Boldini A Defeyter MA Mirshekar-Syahkal D Mehta A 《Bioelectromagnetics》2006,27(3):215-220
Recent studies have indicated that acute exposure to low level radiofrequency (RF) electromagnetic fields generated by mobile phones affects human cognition. However, the relatively small samples used, in addition to methodological problems, make the outcomes of these studies difficult to interpret. In our study we tested a large sample of volunteers (168) using a series of cognitive tasks apparently sensitive to RF exposure (a simple reaction task, a vigilance task, and a subtraction task). Participants performed those tasks twice, in two different sessions. In one session they were exposed to RFs, with half of subjects exposed to GSM signals and the other half exposed to CW signals, while in the other session they were exposed to sham signals. No significant effects of RF exposure on performance for either GSM or CW were found, independent of whether the phone was positioned on the left or on the right side. 相似文献