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ABSTRACT. Sensitivity to cooling stress in the last instar larvae of Galleria mellonella was measured as (a) the number of extra larval moults, (b) the number of larvae retaining the ability to secrete silk, and (c) the number showing arrested development. With respect to (a) and (b) there were considerable differences in sensitivity across the day. A relationship was observed between the number of additional larval moults induced by chilling and the ability of prepupal larvae to spin silk: the periods during the 24 h when the most larvae passed through additional larval moults were periods characterized by the smallest number of larvae capable of spinning, and vice versa. These daily changes were apparently partly independent of developmental age. Daily variations in sensitivity also occurred when larvae of the same age were cooled at different times of day. It is suggested that these rhythms in cold-sensitivity are related to a cold-sensitive rhythm in juvenile hormone secretion, or hormone sensitivity in the tissues. 相似文献
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Desaturation of fatty acids in seeds of higher plants 总被引:5,自引:0,他引:5
Photosynthesizing flax, soybean, and safflower plants were exposed to (14)CO(2) at seed-setting stage for a 1 hr period. Seed was sampled periodically thereafter and the lipids were extracted. A triglyceride-rich fraction was methanolyzed; the resultant methyl esters were analyzed by gas-liquid chromatography and assayed for radioactivity. Of the C(18) unsaturated acids, oleic was the first to acquire radioactivity, which subsequently and successively appeared in linoleic and linolenic acids. The shapes of the radioactivity-time curves provide evidence that consecutive desaturation reactions occur in the seeds of these higher plants. 相似文献
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WIESAW BABIK†† ROGER K. BUTLIN† WILLIAM J. BAKER‡ ALEXANDER S. T. PAPADOPULOS MATTHIEU BOULESTEIX MARIE-CHARLOTTE ANSTETT§ CHRISTIAN LEXER¶ IAN HUTTON VINCENT SAVOLAINEN‡ 《Molecular ecology》2009,18(17):3629-3638
The two species of the palm genus Howea (Arecaceae) from Lord Howe Island, a minute volcanic island in the Tasman Sea, are now regarded as one of the most compelling examples of sympatric speciation, although this view is still disputed by some authors. Population genetic and ecological data are necessary to provide a more coherent and comprehensive understanding of this emerging model system. Here, we analyse data on abundance, juvenile recruitment, pollination mode and genetic variation and structure in both species. We find that Howea forsteriana is less abundant than Howea belmoreana . The genetic data based on amplified fragment length polymorphisms markers indicate similar levels of variation in the two species, despite the estimated census population size of H. belmoreana being three times larger than that of H. forsteriana . Genetic structure within species is low although some weak isolation by distance is detectable. Gene flow between species appears to be extremely limited and restricted to early-generation hybrids – only three admixed individuals, classified as F2s or first generation backcrosses to a parental species, were found among sampled palms. We conclude that speciation in Howea was indeed sympatric, although under certain strict definitions it may be called parapatric. 相似文献