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61.
Susumu Ishii Masaru Wada Shuichi Wakabayashi Hidetsugu Sakai Yoshinori Kubodera Nobuyuki Yamaguchi Motoshi Kikuchi 《Journal of biosciences》1994,19(4):491-502
Once the Japanese ibis, or the Japanese crested ibis, was widely distributed in Asia including Japan, Korea, China and Siberia,
and was not a rare species. However, this species started to disappear over its entire range beginning in the late 19th or
early 20th century. Currently, only a single population of 15–20 individuals survives in wild in Yang Xian, Shaanxi, China.
Several individuals, mostly immature birds, are kept in captivity in Beijing zoo. One of them is an adult male captured in
1981 in Japan and sent to Beijing zoo for breeding two years ago. In Japan, only, a single old female survives in captivity.
Scientists of the Japanese Ibis Preservation Center in Sado Island and Ueno zoo, Tokyo, had attempted several times to breed
Japanese ibises in captivity, but they have failed in all of their attempts. In Beijing zoo, a similar attempt is now being
carried out.
As the basis of an artificial breeding programme of this and other species of birds, the authors have attempted to establish
a noninvasive method for estimation of gonadal activities of birds and also a method to induce a complete series of the ovarian
activity,i.e., ovarian growth, ovulation and oviposition, by means of hormone administration to some species of birds. In this communication,
the author briefly reports recent results of these attempts in addition to results of measurements of gonadotropin levels
in plasma of captive Japanese ibises and white ibises, a closely related species,Threskiornis aethiopicus. 相似文献
62.
Palm mutants in DNA polymerases alpha and eta alter DNA replication fidelity and translesion activity
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Niimi A Limsirichaikul S Yoshida S Iwai S Masutani C Hanaoka F Kool ET Nishiyama Y Suzuki M 《Molecular and cellular biology》2004,24(7):2734-2746
We isolated active mutants in Saccharomyces cerevisiae DNA polymerase alpha that were associated with a defect in error discrimination. Among them, L868F DNA polymerase alpha has a spontaneous error frequency of 3 in 100 nucleotides and 570-fold lower replication fidelity than wild-type (WT) polymerase alpha. In vivo, mutant DNA polymerases confer a mutator phenotype and are synergistic with msh2 or msh6, suggesting that DNA polymerase alpha-dependent replication errors are recognized and repaired by mismatch repair. In vitro, L868F DNA polymerase alpha catalyzes efficient bypass of a cis-syn cyclobutane pyrimidine dimer, extending the 3' T 26000-fold more efficiently than the WT. Phe34 is equivalent to residue Leu868 in translesion DNA polymerase eta, and the F34L mutant of S. cerevisiae DNA polymerase eta has reduced translesion DNA synthesis activity in vitro. These data suggest that high-fidelity DNA synthesis by DNA polymerase alpha is required for genomic stability in yeast. The data also suggest that the phenylalanine and leucine residues in translesion and replicative DNA polymerases, respectively, might have played a role in the functional evolution of these enzyme classes. 相似文献
63.
Ikehara T Yamaguchi H Hosokawa K Houchi H Park KH Minakuchi K Kashimoto H Kitamura M Kinouchi Y Yoshizaki K Miyamoto H 《Biochimica et biophysica acta》2005,1724(1-2):8-16
Exposure of pheochromocytoma (PC 12) cells to a time-varying 1.51 T magnetic field inhibited an increase in the intracellular Ca2+ concentration ([Ca2+]i) induced by addition of caffeine to Ca(2+)-free medium. This inhibition occurred after a 15-min exposure and was maintained for at least 2 h. [Ca2+]i sharply increased in cells loaded with cyclic ADP-ribose, and 2-h exposure significantly suppressed the increase. Addition of ATP induced a transient increase in intracellular Ca2+ release mediated by IP3 receptor, and this increase was strongly inhibited by the exposure. Results indicated that the magnetic field exposure strongly inhibited Ca2+ release mediated by both IP3 and ryanodine receptors in PC 12 cells. However, thapsigargin-induced Ca2+ influx (capacitative Ca2+ entry) across the cell membrane was unaffected. The ATP content was maintained at the normal level during the 2-h exposure, suggesting that ATP hydrolysis was unchanged. Therefore, Mg2+ which is known to be released by ATP hydrolysis and inhibit intracellular Ca2+ release may not relate the exposure-caused inhibition. Eddy currents induced in culture medium appear to change cell membrane properties and indirectly inhibit Ca2+ release from endoplasmic reticulum and other Ca2+ stores in PC 12 cells. 相似文献
64.
We used a power spectrum method to identify periodic patterns in nucleotide sequence, and characterized nucleotide sequences that confer periodicities to prokaryotic and eukaryotic genomes and genomes. A 10-bp periodicity was prevalent in hyperthermophilic bacteria and archaebacteria, and an 11-bp periodicity was prevalent in eubacteria. The 10-bp periodicity was also prevalent in the eukaryotes such as the worm Caenorhabditis elegans. Additionally, in the worm genome, a 68-bp periodicity in chromosome I, a 59-bp periodicity in chromosome II, and a 94-bp periodicity in chromosome III were found. In human chromosomes 21 and 22, approximately 167- or 84-bp periodicity was detected along the entire length of these chromosomes. Because the 167-bp is identical to the length of DNA that forms two complete helical turns in nucleosome organization, we speculated that the respective sequences may correspond to arrays of a special compact form of nucleosomes clustered in specific regions of the human chromosomes. This periodic element contained a high frequency of TGG. TGG-rich sequences are known to form a specific subset of folded DNA structures, and therefore, the sequences might have potential to form specific higher order structures related to the clustered occurrence of a specific form of the speculated nucleosomes. 相似文献
65.
66.
Kurosaki Yoshifumi Imoto Akemi Kawakami Fumitaka Ouchi Motoshi Morita Asuka Yokoba Masanori Takenaka Tsuneo Ichikawa Takafumi Katagiri Masato Nielsen Rikke Ishii Naohito 《Molecular and cellular biochemistry》2022,477(3):689-699
Molecular and Cellular Biochemistry - Bardoxolone methyl [methyl-2-cyano-3, 12-dioxooleana-1, 9(11)dien-28-oate (CDDO-Me)], an activator of the nuclear factor erythroid-derived 2-related factor2... 相似文献
67.
Noriko Miyake Hiroyasu Tsukaguchi Eriko Koshimizu Akemi Shono Satoko Matsunaga Masaaki Shiina Yasuhiro Mimura Shintaro Imamura Tomonori Hirose Koji Okudela Kandai Nozu Yuko Akioka Motoshi Hattori Norishige Yoshikawa Akiko Kitamura Hae?Il Cheong Shoji Kagami Michiaki Yamashita Atsushi Fujita Satoko Miyatake Yoshinori Tsurusaki Mitsuko Nakashima Hirotomo Saitsu Kenichi Ohashi Naoko Imamoto Akihide Ryo Kazuhiro Ogata Kazumoto Iijima Naomichi Matsumoto 《American journal of human genetics》2015,96(4):555-564
We investigated complex genomic rearrangements (CGRs) consisting of triplication copy-number variants (CNVs) that were accompanied by extended regions of copy-number-neutral absence of heterozygosity (AOH) in subjects with multiple congenital abnormalities. Molecular analyses provided observational evidence that in humans, post-zygotically generated CGRs can lead to regional uniparental disomy (UPD) due to template switches between homologs versus sister chromatids by using microhomology to prime DNA replication—a prediction of the replicative repair model, MMBIR. Our findings suggest that replication-based mechanisms might underlie the formation of diverse types of genomic alterations (CGRs and AOH) implicated in constitutional disorders. 相似文献
68.
69.
Kajiho Y Harita Y Kurihara H Horita S Matsunaga A Tsurumi H Kanda S Sugawara N Miura K Sekine T Hattori S Hattori M Igarashi T 《The FEBS journal》2012,279(17):3010-3021
The slit diaphragm (SD) is an intercellular junction between renal glomerular epithelial cells (podocytes) that is essential for permselectivity in glomerular ultrafiltration. The SD components, nephrin and Neph1, assemble a signaling complex in a tyrosine phosphorylation dependent manner, and regulate the unique actin cytoskeleton of podocytes. Mutations in the NPHS1 gene that encodes nephrin cause congenital nephrotic syndrome (CNS), which is characterized by the loss of the SD and massive proteinuria. Recently, we have identified the expression of the transmembrane glycoprotein signal regulatory protein α (SIRPα) at the SD. In the present study, we analyzed the expression of SIRPα in developing kidneys, in kidneys from CNS patients and in proteinuric rat models. The possibility that SIRPα interacts with known SD proteins was also investigated. SIRPα was concentrated at the SD junction during the maturation of intercellular junctions. In the glomeruli of CNS patients carrying mutations in NPHS1, where SD formation is disrupted, the expression of SIRPα as well as Neph1 and nephrin was significantly decreased, indicating that SIRPα is closely associated with the nephrin complex. Indeed, SIRPα formed hetero-oligomers with nephrin in cultured cells and in glomeruli. Furthermore, the cytoplasmic domain of SIRPα was highly phosphorylated in normal glomeruli, and its phosphorylation was dramatically decreased upon podocyte injury in?vivo. Thus, SIRPα interacts with nephrin at the SD, and its phosphorylation is dynamically regulated in proteinuric states. Our data provide new molecular insights into the phosphorylation events triggered by podocyte injury. Structured digital abstract ? Sirp-alpha?physically interacts?with?Nephrin?by?anti bait coimmunoprecipitation?(View interaction) ? Sirp-alpha?physically interacts?with?Nephrin?by?anti tag coimmunoprecipitation?(View interaction). 相似文献
70.
Suzuki M Lee DY Inyamah N Stadtman TC Tjandra N 《The Journal of biological chemistry》2008,283(38):25936-25943
Selenium is an important nutrient. The lack of selenium will suppress expression of various enzymes that will lead to cell abnormality and diseases. However, high concentrations of free selenium are toxic to the cell because it adversely affects numerous cell metabolic pathways. In Methanococcus vannielii, selenium transport in the cell is established by the selenium-binding protein, SeBP. SeBP sequesters selenium during transport, thus regulating the level of free selenium in the cell, and delivers it specifically to the selenophosphate synthase enzyme. In solution, SeBP is an oligomer of 8.8-kDa subunits. It is a symmetric pentamer. The solution structure of SeBP was determined by NMR spectroscopy. Each subunit of SeBP is composed of an alpha-helix on top of a 4-stranded twisted beta-sheet. The stability of the five subunits stems mainly from hydrophobic interactions and supplemented by hydrogen bond interactions. The loop containing Cys(59) has been shown to be important for selenium binding, is flexible, and adopts multiple conformations. However, the cysteine accessibility is restricted in the structure, reducing the possibility of the binding of free selenium readily. Therefore, a different selenium precursor or other factors might be needed to facilitate opening of this loop to expose Cys(59) for selenium binding. 相似文献