全文获取类型
收费全文 | 1011篇 |
免费 | 48篇 |
国内免费 | 1篇 |
出版年
2023年 | 2篇 |
2022年 | 7篇 |
2021年 | 13篇 |
2020年 | 6篇 |
2019年 | 13篇 |
2018年 | 14篇 |
2017年 | 14篇 |
2016年 | 19篇 |
2015年 | 22篇 |
2014年 | 28篇 |
2013年 | 52篇 |
2012年 | 54篇 |
2011年 | 54篇 |
2010年 | 36篇 |
2009年 | 38篇 |
2008年 | 72篇 |
2007年 | 71篇 |
2006年 | 62篇 |
2005年 | 66篇 |
2004年 | 45篇 |
2003年 | 51篇 |
2002年 | 41篇 |
2001年 | 29篇 |
2000年 | 27篇 |
1999年 | 22篇 |
1998年 | 14篇 |
1997年 | 5篇 |
1996年 | 8篇 |
1995年 | 9篇 |
1994年 | 4篇 |
1993年 | 9篇 |
1992年 | 21篇 |
1991年 | 17篇 |
1990年 | 19篇 |
1989年 | 15篇 |
1988年 | 7篇 |
1987年 | 2篇 |
1986年 | 8篇 |
1985年 | 14篇 |
1984年 | 10篇 |
1983年 | 9篇 |
1982年 | 5篇 |
1981年 | 7篇 |
1980年 | 3篇 |
1979年 | 6篇 |
1977年 | 3篇 |
1975年 | 3篇 |
1974年 | 1篇 |
1971年 | 1篇 |
1969年 | 1篇 |
排序方式: 共有1060条查询结果,搜索用时 15 毫秒
21.
Tissue and subcellular distributions of an inhibitory GDP/GTP exchange protein (GDI) for smg p25A by use of its antibody 总被引:3,自引:0,他引:3
H Nonaka K Kaibuchi K Shimizu J Yamamoto Y Takai 《Biochemical and biophysical research communications》1991,174(2):556-563
We have recently purified from bovine brain cytosol to near homogeneity a GDP/GTP exchange protein for smg p25A, named smg p25A GDI, that inhibits the dissociation of GDP from and the subsequent binding of GTP to smg p25A. In the present study, we made an antiserum against smg p25A GDI and studied its tissue distribution in rat and its subcellular distribution in rat cerebrum by use of this antiserum. smg p25A GDI was found in secretory cells with both regulated and constitutive secretion types. Since smg p25A was previously found in only secretory cells with a regulated secretion type, this result suggests that small GTP-binding proteins different from smg p25A but recognized by smg p25A GDI are present in secretory cells with a constitutive secretion type, and that smg p25A GDI is involved in both regulated and constitutive secretory processes. In subcellular fractionation analysis of rat cerebrum, smg p25A GDI was mostly found in the cytosol fraction of neuron body and synaptosome. In synaptosome, it was mainly found in the synaptic cytosol. 相似文献
22.
A microbial sensor system consisting of the bacterium (Alteromonas putrefaciens) immobilized within membranes, a flow cell, an oxygen electrode, peristaltic pumps, a buffer tank, a thermostatically controlled bath and a recorder, was constructed for the nondestructive quality evaluation of bluefin tuna. The chemical compounds on fish meat surfaces which are the indicators of fish meat quality were rapidly determined by using the proposed sensor system. Fish meat quality was determined from the rate of current decrease of the sensor. Good correlations were obtained between fish meat quality and sensor response. One assay could be completed within one minute. 相似文献
23.
24.
25.
Single crystals of NADPH-adrenodoxin oxidoreductase were grown in 50 mM potassium phosphate (pH 7.4) containing 5% glycerol and ammonium sulfate. The crystals are monoclinic, belong to space group P21 and have dimensions of a= 83.4 A, b = 62.6 A, c = 59.3 A, alpha = gamma = 90 degrees, and beta = 107.1 degrees. There is one molecule per asymmetric unit. 相似文献
26.
7-O-Galloyl-(+)-catechin and 3-O-galloylprocyanidin B-3, along with gambiriins A-1 and B-3 and four polygalloylglucoses, have been isolated fro 相似文献
27.
Experimental acute degenerative changes in skeletal muscle accompanied by a drastic increase in cathepsins B&L were induced in rats by intramuscular injection of a local anesthetic, bupivacaine. Cathepsins B&L have been implicated in the rapid disappearance of muscle fibers. Degenerating muscle showed a spotty fluorescence when stained with antibodies against cathepsin B, indicating that the increased cathepsin B did not originate from the muscle itself, but from invading phagocytes. We report here results showing that cathepsin B of nonmuscle cell origin is involved in the breakdown of myofibrillar proteins in acute bupivacaine-induced muscle degeneration. 相似文献
28.
Masahide Nonaka 《Phytochemistry》1985,25(1):73-75
Eight saponins were isolated from alfalfa roots (Medicago sativa). The sensitivity of Trichoderma viride to the saponin varied with the individual saponin isolate. Seven isolates appeared to contain the aglycone, medicagenic acid, and while the other did not, it inhibited the growth of the fungus at higher concentrations than the other isolates. One pair and a triplet of saponins with divergent Rfs evoked near identical biological responses suggesting structural similarity toxic to T. viride. 相似文献
29.
Takahashi Yumiko Sarkar Juli Yamada Jumpei Matsunaga Yutaka Nonaka Yudai Banjo Mai Sakaguchi Ryo Shinya Terunaga Hatta Hideo 《Journal of physiology and biochemistry》2021,77(3):469-480
Journal of Physiology and Biochemistry - To identify factors that influence post-exercise muscle glycogen repletion, we compared the glycogen recovery after level running with downhill running, an... 相似文献
30.
Hidetaka Sugihara Takatsugu Ishimoto Masayuki Watanabe Hiroshi Sawayama Masaaki Iwatsuki Yoshifumi Baba Yoshihiro Komohara Motohiro Takeya Hideo Baba 《PloS one》2013,8(11)
Bmi1 is overexpressed in a variety of human cancers including gastrointestinal cancer. The high expression level of Bmi1 protein is associated with poor prognosis of gastrointestinal cancer patients. On the other hand, tumor-associated macrophages (TAMs) contribute to tumor growth, invasion, and metastasis by producing various mediators in the tumor microenvironment. The aim of this study was to investigate TAM-mediated regulation of Bmi1 expression in gastrointestinal cancer. The relationship between TAMs and Bmi1 expression was analyzed by immunohistochemistry and quantitative real-time PCR (qRT-PCR), and results showed a positive correlation with tumor-infiltrating macrophages (CD68 and CD163) and Bmi1 expression in cancer cells. Co-culture with TAMs triggered Bmi1 expression in cancer cell lines and enhanced sphere formation ability. miRNA microarray analysis of a gastric cancer cell line co-cultured with macrophages was conducted, and using in silico methods to analyze the results, we identified miR-30e* as a potential regulator of Bmi1 expression. Luciferase assays using miR-30e* mimic revealed that Bmi1 was a direct target for miR-30e* by interactions with the putative miR-30e* binding sites in the Bmi1 3′ untranslated region. qRT-PCR analysis of resected cancer specimens showed that miR-30e* expression was downregulated in tumor regions compared with non-tumor regions, and Bmi1 expression was inversely correlated with miR-30e* expression in gastric cancer tissues, but not in colon cancer tissues. Our findings suggest that TAMs may cause increased Bmi1 expression through miR-30e* suppression, leading to tumor progression. The suppression of Bmi1 expression mediated by TAMs may thus represent a possible strategy as the treatment of gastrointestinal cancer. 相似文献