全文获取类型
收费全文 | 938篇 |
免费 | 57篇 |
国内免费 | 1篇 |
专业分类
996篇 |
出版年
2024年 | 4篇 |
2023年 | 11篇 |
2022年 | 38篇 |
2021年 | 55篇 |
2020年 | 35篇 |
2019年 | 59篇 |
2018年 | 56篇 |
2017年 | 29篇 |
2016年 | 51篇 |
2015年 | 49篇 |
2014年 | 54篇 |
2013年 | 69篇 |
2012年 | 84篇 |
2011年 | 60篇 |
2010年 | 35篇 |
2009年 | 31篇 |
2008年 | 32篇 |
2007年 | 32篇 |
2006年 | 25篇 |
2005年 | 32篇 |
2004年 | 27篇 |
2003年 | 22篇 |
2002年 | 26篇 |
2001年 | 9篇 |
2000年 | 5篇 |
1999年 | 2篇 |
1997年 | 5篇 |
1996年 | 3篇 |
1995年 | 7篇 |
1994年 | 3篇 |
1993年 | 2篇 |
1992年 | 2篇 |
1991年 | 3篇 |
1990年 | 2篇 |
1989年 | 4篇 |
1985年 | 3篇 |
1984年 | 4篇 |
1982年 | 2篇 |
1977年 | 2篇 |
1976年 | 1篇 |
1975年 | 2篇 |
1974年 | 2篇 |
1972年 | 1篇 |
1970年 | 2篇 |
1969年 | 2篇 |
1967年 | 4篇 |
1965年 | 1篇 |
1962年 | 1篇 |
1961年 | 1篇 |
1960年 | 1篇 |
排序方式: 共有996条查询结果,搜索用时 9 毫秒
21.
Thirty-eight genera and 81 species of fungi were isolated and identified from 120 samples of 24 kinds of spices collected from different places at Assiut Governorate, Egypt. Predominant genera wereAspergillus (25 species) andPenicillium (7 species) of whichA. flavus, A. niger, A. ochraceus, A. fumigatus, A. flavus var.columnaris, A. terreus, P. chrysogenum andP. corylophilum were the most commonly occurring. 相似文献
22.
Ansari-Lari M Saadat M Hadi N 《Biochemical and biophysical research communications》2003,312(2):299-302
Workers in the petroleum distribution trades experience relatively low-level exposures to gasoline vapors whose consequences have not been fully elucidated. The purpose of this study was to investigate changes in the hematological parameters among filling station workers who were occupationally exposed to gasoline. The target group for the study consisted of 41 workers from eight filling stations of Shiraz (south of Iran). The control group consisted of 27 healthy subjects matched for age and sex from general population. The complete blood count analysis was done in one laboratory. Using PCR-based method, the genotypes of glutathione S-transferase T1 (GSTT1) and M1 (GSTM1) were determined. Workers were divided into three exposure groups according to employment history: duration less than 1 year, 1-5 years, and more than 5 years. Comparison was performed using Kruskal-Wallis test. In the individuals with the presence of both GSTT1 and GSTM1 functional alleles, comparison between four exposure groups revealed no significant difference for studied hematological variables. There were statistically significant differences between study groups, with only one functional allele, either GSTT1 or GSTM1, for relative number of lymphocytes (chi(2)=9.147, df=3, P=0.027) and neutrophils (chi(2)=9.951, df=3, and P=0.019), and absolute number of lymphocytes (chi(2)=9.135, df=3, and P=0.028), and RBC (chi(2)=10.586, df=3, and P=0.014). These findings could indicate the possible protective effect of concurrent presence of GSTM1 and GSTT1 enzymes on the hematopoietic system of filling station workers. 相似文献
23.
Abu Hena Mostafa Kamal Ki-Hyun Kim Kwang-Hyun Shin Hyung-Seok Seo Hisashi Tsujimoto Hwa-Young Heo Jong-Soon Choi Chul-Soo Park Sun-Hee Woo 《Journal of Plant Biology》2009,52(6):533-542
Glutenin is a major determinant of baking performance and viscoelasticity, which are responsible for high-quality bread with
a light porous crumb structure of a well-leavened loaf. We analyzed the diversity of glutenin genes from six wheat cultivars
(Korean cvs. Keumgang and Jinpum, Chinese cvs. China-108 and Yeonnon-78, and Japanese cvs. Norin-61 and Kantou-107). Glutenins
contain two types of isoforms such as high molecular weight glutenin subunit (HMW-GS) and low molecular weight glutenin subunit
(LMW-GS). Glutenin fractions were extracted from wheat endosperm using Osborne solubility method. A total of 217 protein spots
were separated on two-dimensional gel electrophoresis with isoelectric focusing (wide range of pH 3–10). The proteins spots
were subjected to tryptic digestion and identified by matrix assisted laser desorption/ionization–time of flight mass spectrometry.
HMW-GS (43 isoforms) and LMW-GS (seven isoforms) are directly responsible for producing high-quality bread and noodles. Likewise,
all the seed storage proteins are digested to provide nutrients for the embryo during seed germination and seedling growth.
We identified the diverse glutenin subunits in wheat cultivars and compared the gluten isoforms among different wheat cultivars
according to quality. This work gives an insight on the quality improvement in wheat crop. 相似文献
24.
Meamar AR Guyot K Certad G Dei-Cas E Mohraz M Mohebali M Mohammad K Mehbod AA Rezaie S Rezaian M 《Applied and environmental microbiology》2007,73(3):1033-1035
Isolates of Cryptosporidium spp. from human and animal hosts in Iran were characterized on the basis of both the 18S rRNA gene and the Laxer locus. Three Cryptosporidium species, C. hominis, C. parvum, and C. meleagridis, were recognized, and zoonotically transmitted C. parvum was the predominant species found in humans. 相似文献
25.
26.
Zahra Rahmani Chang‐Jun Kim Mostafa Ghafouri Moghaddam Ehsan Rakhshani 《Entomological Research》2019,49(9):409-415
Occurrence of the family Ismaridae Thomson is recorded for the first time from Iran, represented by a single species, Ismarus rugulosus Förster, 1850. A series of diagnostic characters and morphometric ratios for this species is presented, as well as a distribution map throughout the Holarctic region. 相似文献
27.
The genes involved in DNA repair system play a crucial role in the protection against mutations. It has been hypothesized
that functional deficiencies in highly conserved DNA repair processes resulting from polymorphic variation may increase genetic
susceptibility to breast cancer (BC). The aim of the present study was to evaluate the association of genetic polymorphisms
in 2 DNA repair genes, XPD (Asp312Asn) and XRCC1 (A399G), with BC susceptibility. We further investigated the potential combined
effect of these DNA repair variants on BC risk. Both XPD (xeroderma pigmentosum group D) and XRCC1 (X-ray repair cross-complementing
group 1) polymorphisms were characterized in 100 BC Egyptian females and 100 healthy women who had no history of any malignancy
by amplification refractory mutation system-polymerase chain reaction (ARMS-PCR) method and PCR with confronting two-pair
primers (PCR-CTPP), using DNA from peripheral blood in a case control study. Our results revealed that the frequencies of
AA genotype of XPD codon 312 polymorphism were significantly higher in the BC patients than in the normal individuals (P ≤ 0.003), and did not observe any association between the XRCC1 Arg399Gln polymorphism and risk of developing BC. Also, no
association between both XPD Asp312Asn and XRCC1 A399G polymorphisms and the clinical characteristics of disease. Finally,
the combination of AA(XPD) + AG(XRCC1) were significantly associated with BC risk. Our results suggested that, XPD gene is
an important candidate gene for susceptibility to BC. Also, gene–gene interaction between XPD(AA) + XRCC1(AG) polymorphism
may be associated with increased risk of BC in Egyptian women. 相似文献
28.
M Rahimnejad GD Najafpour AA Ghoreyshi F Talebnia GC Premier G Bakeri JR Kim SE Oh 《Journal of microbiology (Seoul, Korea)》2012,50(4):575-580
Microbial fuel cells (MFCs) have been shown to be capable of clean energy production through the oxidation of biodegradable organic waste using various bacterial species as biocatalysts. In this study we found Saccharomyces cerevisiae, previously known electrochemcially inactive or less active species, can be acclimated with an electron mediator thionine for electrogenic biofilm formation in MFC, and electricity production is improved with facilitation of electron transfer. Power generation of MFC was also significantly increased by thionine with both aerated and non-aerated cathode. With electrochemically active biofilm enriched with swine wastewater, MFC power increased more significantly by addition of thionine. The optimum mediator concentration was 500 mM of thionine with S. cerevisae in MFC with the maximum voltage and current generation in the microbial fuel cell were 420 mV and 700 mA/m(2), respectively. Cyclic voltametry shows that thionine improves oxidizing and reducing capability in both pure culture and acclimated biofilm as compared to non-mediated cell. The results obtained indicated that thionine has great potential to enhance power generation from unmediated yeast or electrochemically active biofilm in MFC. 相似文献
29.
The D-3-hydroxybutyrate dehydrogenase (BDH) (EC 1.1.1.30) from liver jerboa (Jaculus orientalis), a ketone body converting enzyme in mitochondria, in two populations of mitochondria (heavy and light) has been studied in different jerboa states (euthermic, prehibernating and hibernating). The results reveal: (1) important variations between states in terms of ketones bodies, glucose and lipid levels; (2) significant differences between the BDH of the two mitochondrial populations in term of protein expression and kinetic properties. These results suggest that BDH leads an important conformational change depending on the physiological state of jerboa. This BDH structural change could be the consequence of the lipid composition modifications in inner mitochondrial membrane leading to changes in BDH catalytic properties. 相似文献
30.
An isoenzyme analysis was performed on 109 individuals from 11 populations of Juniperus excelsa complex collected from Iran. A total of 61 allozymes from five enzyme systems were scored. The mean numbers of bands per presumed isoenzyme, ranged from 2.07 to 3.23. The value of Euclidean distance ranged from 1.920 to 4.411. The average value of Shannon diversity index (H′) estimated for each population ranged from 0.317 in the population sampled from ‘Fasa’ to 1.053 in the population of ‘Golestan’, and the average H′ value for each enzyme system ranged from 0.031 (SOD-3) to 1.670 (PPO-1). To reveal the relationships among these populations, a cluster analysis based on Euclidean distances was performed. The results of the analysis suggest recognizing three taxa in the complex in Iran: Juniperus polycarpos subsp. polycarpos, Juniperus polycarpos subsp. turcomanica, and one cryptic species not recorded from Iran until now. Moreover, the analysis presented, does not confirm the occurrence of J. excelsa in Iran. 相似文献