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121.
Iron-responsive regulation of biofilm formation in staphylococcus aureus involves fur-dependent and fur-independent mechanisms
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We have shown that Staphylococcus aureus biofilm production is induced in iron-restricted conditions and is repressed by iron via a Fur-independent mechanism, while Fur has both positive and negative regulatory roles in low iron. Furthermore, there is no significant increase in polymeric N-acetylglucosamine polysaccharide expression to account for induction of biofilms in low iron. 相似文献
122.
AIMS: To study the role of the indigenous yeast flora in traditional Irish cider fermentations. METHODS AND RESULTS: Wallerstein laboratory nutrient agar supplemented with biotin, ferric ammonium citrate, calcium carbonate and ethanol was employed together with PCR-restriction fragment length polymorphism analysis of the region spanning the internal transcribed spacers (ITS1 and ITS2) and the 5.8S rRNA gene in the identification of indigenous yeasts at the species level, from traditional Irish cider fermentations. By combining the molecular approach and the presumptive media it was possible to distinguish between a large number of yeast species, and to track them within cider fermentations. The Irish cider fermentation process can be divided into three sequential phases based on the predominant yeast type present. Kloeckera/Hanseniaspora uvarum type yeasts predominate in the initial 'fruit yeast phase'. Thereafter Saccharomyces cerevisiae type yeast dominate in the 'fermentation phase', where the alcoholic fermentation takes place. Finally the 'maturation phase' which follows, is dominated by Dekkera and Brettanomyces type yeasts. H. uvarum type yeast were found to have originated from the fruit. Brettanomyces type yeast could be traced back to the press house, and also to the fruit. The press house was identified as having high levels of S. cerevisiae type yeast. A strong link was noted between the temperature profile of the cider fermentations, which ranged from 22 to 35 degrees C and the yeast strain population dynamics. CONCLUSIONS: Many different indigenous yeast species were identified. The mycology of Irish cider fermentations appears to be very similar to that which has previously been reported in the wine industry. SIGNIFICANCE AND IMPACT OF THE STUDY: This study has allowed us to gain a better understanding of the role of indigenous yeast species in 'Natural' Irish cider fermentations. 相似文献
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Morrissey JP Walsh UF O'Donnell A Moënne-Loccoz Y O'Gara F 《Antonie van Leeuwenhoek》2002,81(1-4):599-606
The major growth seen in the biotechnology industry in recent decades has largely been driven by the exploitation of genetic
engineering techniques. The initial benefits have been predominantly in the biomedical area, with products such as vaccines
and hormones that have received broad public approval. In the environmental biotechnology and industrial ecology sectors,
biotechnology has the potential to make significant advances through the use of genetically modified (GM) microbial inoculants
that can reduce agri-chemical usage or remediate polluted environments. Although many GM inoculants have been developed and
tested under laboratory conditions, commercial exploitation has lagged behind. Here, we review scientific and regulatory requirements
that must be satisfied as part of that exploitation process. Particular attention is paid to new European Union (EU) regulations
(Directives) that govern the testing and release of genetically modified organisms and microbial plant protection inoculants
in the EU. With regard to the release of GM inoculants, the impact of the inoculant and the fate of modified genes are important
concerns. Long term monitoring of release sites is necessary to address these issues. Data are reported from the monitoring
of a site 6 years after release of GM Sinorhizobium meliloti strains. It was found that despite the absence of a host plant, the GM strains persisted in the soil for at least 6 years.
Horizontal transfer and microevolution of a GM plasmid between S. meliloti strains was also observed. These data illustrate the importance of assessing the long-term persistence of GM inoculants.
This revised version was published online in August 2006 with corrections to the Cover Date. 相似文献
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Horuk R Clayberger C Krensky AM Wang Z Grone HJ Weber C Weber KS Nelson PJ May K Rosser M Dunning L Liang M Buckman B Ghannam A Ng HP Islam I Bauman JG Wei GP Monahan S Xu W Snider RM Morrissey MM Hesselgesser J Perez HD 《The Journal of biological chemistry》2001,276(6):4199-4204
Chemokines like RANTES appear to play a role in organ transplant rejection. Because RANTES is a potent agonist for the chemokine receptor CCR1, we examined whether the CCR1 receptor antagonist BX471 is efficacious in a rat heterotopic heart transplant rejection model. Treatment of animals with BX471 and a subtherapeutic dose of cyclosporin (2.5 mg/kg), which is by itself ineffective in prolonging transplant rejection, is much more efficacious in prolonging transplantation rejection than animals treated with either cyclosporin or BX471 alone. We have examined the mechanism of action of the CCR1 antagonist in in vitro flow assays over microvascular endothelium and have discovered that the antagonist blocks the firm adhesion of monocytes triggered by RANTES on inflamed endothelium. Together, these data demonstrate a significant role for CCR1 in allograft rejection. 相似文献
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Mary Cloud B. Ammons Kathryn Morrissey Brian P. Tripet James T. Van Leuven Anne Han Gerald S. Lazarus Jonathan M. Zenilman Philip S. Stewart Garth A. James Valérie Copié 《PloS one》2015,10(5)
Chronic, non-healing wounds contribute significantly to the suffering of patients with co-morbidities in the clinical population with mild to severely compromised immune systems. Normal wound healing proceeds through a well-described process. However, in chronic wounds this process seems to become dysregulated at the transition between resolution of inflammation and re-epithelialization. Bioburden in the form of colonizing bacteria is a major contributor to the delayed headlining in chronic wounds such as pressure ulcers. However how the microbiome influences the wound metabolic landscape is unknown. Here, we have used a Systems Biology approach to determine the biochemical associations between the taxonomic and metabolomic profiles of wounds colonized by bacteria. Pressure ulcer biopsies were harvested from primary chronic wounds and bisected into top and bottom sections prior to analysis of microbiome by pyrosequencing and analysis of metabolome using 1H nuclear magnetic resonance (NMR) spectroscopy. Bacterial taxonomy revealed that wounds were colonized predominantly by three main phyla, but differed significantly at the genus level. While taxonomic profiles demonstrated significant variability between wounds, metabolic profiles shared significant similarity based on the depth of the wound biopsy. Biochemical association between taxonomy and metabolic landscape indicated significant wound-to-wound similarity in metabolite enrichment sets and metabolic pathway impacts, especially with regard to amino acid metabolism. To our knowledge, this is the first demonstration of a statistically robust correlation between bacterial colonization and metabolic landscape within the chronic wound environment. 相似文献
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Kennedy J O'Leary ND Kiran GS Morrissey JP O'Gara F Selvin J Dobson AD 《Journal of applied microbiology》2011,111(4):787-799
Marine ecosystems are home to bacteria which are exposed to a wide variety of environmental conditions, such as extremes in temperature, salinity, nutrient availability and pressure. Survival under these conditions must have necessitated the adaptation and the development of unique cellular biochemistry and metabolism by these microbes. Thus, enzymes isolated from these microbes have the potential to possess quite unique physiological and biochemical properties. This review outlines a number of function-based metagenomic approaches which are available to screen metagenomic libraries constructed from marine ecosystems to facilitate the exploitation of some of these potentially novel biocatalysts. Functional screens to isolate novel cellulases, lipases and esterases, proteases, laccases, oxidoreductases and biosurfactants are described, together with approaches which can be employed to help overcome some of the typical problems encountered with functional metagenomic-based screens. 相似文献