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91.
M Iu Gololobov I P Morozova T L Voiushina E A Timokhina V M Stepanov 《Biokhimii?a (Moscow, Russia)》1991,56(2):230-240
A comparative study of the hydrolysis of various p-nitroanilide substrates (Z-A2-A1-pNA, Z-A3-A2-A1-pNA, and Z-A4-A3-A2-A1-pNA, where A1-An are various amino acid residues, Z is the benzoyloxycarbonylic group and pNA is the p-nitroanilide group), catalyzed by serine proteinase from Bacillus subtilis strain 72, was carried out. It was found that depending on the substrate structure, the hydrolysis may involve both the peptide-p-nitroaniline and the amino acid-amino acid bonds. A kinetic analysis of substrate hydrolysis occurring simultaneously at these two bonds was carried out. The physico-chemical meaning of the kinetic parameters of the given scheme was determined. The quantitative estimation of the enzyme specificity with respect to both hydrolyzing bonds can be found by using the parameters calculated during the analysis of the kinetic curve of p-nitroaniline production. It was found that according to their specificity the amino acid residues at position A1 can be arranged in the following order: L-Leu greater than P-Phe greater than L-Ile greater than L-Ala. The beta-branched amino acid residues, L-Val and L-Ile, do not bind to subsite S1. If these residues occupy position A1, the substrate splitting occurs exclusively between residues A1 and A2. The tetrapeptide N-protected p-nitroanilide substrates are also hydrolyzed at this bond. Partial hydrolysis of the amino acid-amino acid bond between residues A1 and A2 occurs in two cases: i) when residue A1 is loosely bound to subsite S1 and/or, ii) when residue A2 is firmly bound to subsite S1. 相似文献
92.
93.
The experimental data are presented concerning the effect of polyribonucleotides on the immunologic and colony forming ability of bone marrow or irradiated mice. All the compounds under study exhibited a pronounced, but to a different degree, colony-forming and immunostimulating action. The comparative study of the influence of polyribonucleotides on the number of endogenous colonies and antibody-forming cells showed an inverse relationship between these parameters: The preparations exerting the most pronounced immunostimulating effect had an insignificant colony-forming action and vice versa. This is evidently indicative of the capacity of these preparations to turn the differentiation of haemopoietic stem cells towards the immunopoiesis. 相似文献
94.
95.
M V Ivanov B A Lianda-Geller A D Morozova V S Pirogov G V Samsonov 《Prikladnaia biokhimiia i mikrobiologiia》1986,22(6):806-809
The effect of heat treatment used in the course of tuberculin production by Mycobacteria tuberculosis cells on the structure and biological activity of tuberculin was studied. Methods of preparative and analytical gel-filtration were used. The largest quantity of the biologically active component was obtained after 2-hour heat treatment of the mycobacteria culture. 相似文献
96.
97.
Morozova MP Gavrilova SA Zemtsova LV Pogodina LS Postnikov AB Chentsov IuS 《Rossi?skii fiziologicheski? zhurnal imeni I.M. Sechenova / Rossi?skaia akademiia nauk》2012,98(2):269-282
Myocarditis development was investigated after immunization rats with single subcutaneous injection of cardiac myosin (800 microg/kg) with incomplete Freund's adjuvant (IFA) (M + IFA group). Control group received equal volume of IFA alone or nothing (intact group). On days 4, 14, and 21 after injection, light and electron microscopy of heart sections, morphometric analysis, estimation of proinflammatory cytokines (IL-1p, IL-6, VEGF, TNFa and iNOS) expression were used to evaluate inflammatory response in myocardium. In addition, we estimated cardiac myosin antibody levels in blood serum and nitrite and nitrate levels in blood serum. Our data showed that immunization with cardiac myosin combined with IFA led to inflammatory response in the rat myocardium. Acute inflammation (i.e. lymphocyte infiltration of myocardium and increase of proinflammatory cytokines level) in M + IFA group occurred on 21 days after immunization. 相似文献
98.
Kharitonova MA Vershinina VI Morozova OV Znamenskaia LV 《Molekuliarnaia genetika, mikrobiologiia i virusologiia》2006,(4):15-19
Guanylspecific ribonucleases from B. intermedius (binase) and B.pumilus (RNase Bpu) are structural and functional homologues, and their biosynthesis is subjected to the same laws. At the same time, there are essential differences in the expression efficiency of binase and RNase Bpu genes. This was first suggested to be due to differences in nucleotide sequences of promoters of the genes. Therefore, we constructed plasmids changing each different nucleotide in binase promoter for corresponding one from RNase Bpu and vise versa. It was found that the difference in RNase Bpu and binase expression was due to the only nucleotide in RNase Bpu promoter. 相似文献
99.
M. V. Semenova O. A. Sinitsyna V. V. Morozova E. A. Fedorova A. V. Gusakov O. N. Okunev L. M. Sokolova A. V. Koshelev T. V. Bubnova Yu. P. Vinetskii A. P. Sinitsyn 《Applied Biochemistry and Microbiology》2006,42(6):598-602
A new enzyme preparation of fungal pectin lyase (EC 4.2.2.10) was shown to be useful for the production of cranberry juice and clarification of apple juice in the food industry. A comparative study showed that the preparation of pectin lyase is competitive with commercial pectinase products. The molecular weight of homogeneous pectin lyase was 38 kDa. Properties of the homogeneous enzyme were studied. This enzyme was most efficient in removing highly esterified pectin. 相似文献
100.
Kosse LV Lebedeva SA Cherniavskaia AS Nekliaev VN Markina OV Morozova IV Eremenko NS Sal'nikova OI 《Zhurnal mikrobiologii, epidemiologii, i immunobiologii》2006,(3):76-81
Components of the capsule antigen (Baker), described early as F17, F18, F43, Flp, which positively reacts with commercial poly- and monoclonal antifractions plague diagnosticums were studied. Differences and their impacts on vaccine bacteria survival within peritoneal macrophages, guinea pigs and protection of white mice after immunization and fast protection against plague were shown. Hemolytic, cytolytic and hemo- and cytoagglutinations activities of lipoprotein (Flp) and capacity of glycoproteins F17 and F43 for induction of hemo- and cytoagglutinations were detected. Similarity of properties of enumerated components and lectins and their role in infection development and immunogenesis are discussed. 相似文献