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581.
582.
I. I. Ermakova T. A. Chertkova A. L. Mokrushin G. A. Sakuta A. V. Romaniouk V. I. Morozov 《Cell and Tissue Biology》2008,2(5):538-545
Proteoglycans were isolated from the extracellular matrix (ECM) of L6J1 rat myoblasts; their influence on myoblast adhesion has been studied. Proteoglycan digestion with chondroitinase AC and heparinase III, which degrade polysaccharide moieties, has revealed that chondroitin sulfate proteoglycans are a major class of myoblast extracellular matrix proteoglycans. Electrophoresis of enzymatically processed proteoglycans was used to examine their core proteins. Myoblast adhesion was suppressed by proteoglycans or a mixture of proteoglycans and a fibronectin-extracellular matrix. Myoblast adhesion to a substrate composed of fibronectin and proteoglycans is restored after the substrate was treated with chondroitinase AC. In conclusion, proteoglycans of L6J1 rat myoblast ECMs were isolated and purified. Chondroitin sulfate proteoglycans are a major class of proteoglycans. Isolated proteoglycans suppressed myoblast adhesion; the effect was mediated by polysaccharide moieties of proteoglycans. 相似文献
583.
N. V. Kosheleva I. V. Ilina K. V. Kozhina I. M. Zurina A. E. Roskova A. A. Gorkun A. V. Ovchinnikov M. B. Agranat S. G. Morozov I. N. Saburina 《Russian Journal of Developmental Biology》2017,48(1):56-64
In this study, modern techniques of laser microsurgery of cell spheroids have been used to develop a new simple, reproducible model for studying the mechanisms of repair and regeneration in vitro. Nanosecond laser pulses were applied to perform a microdissection of the outer and the inner zones of the spheroids from dermal fibroblasts. To achieve effective dissection and preservation of spheroid viability, the optimal parameters were chosen: 355 nm wavelength, 100 Hz frequency, 2 ns pulse duration, laser pulses in the range of 7–9 μJ. After microdissection, we observed injury of the spheroids: the edges of the wound surface opened and the angular opening reached a value of more than 180°. As early as during the first hour after spheroid microdissection with laser radiation, the surviving cells changed their shape: cells on the spheroid surface and directly in the damaged area became rounded. One day after microdissection, the structure of the spheroids began to partially recover, the cells in the surface layers began to take the original flattened shape; debris of dead damaged cells and their fragments was gradually cleared from the spheroid composition. In the proposed model, the first data on stimulation of structure recovery of injured spheroids from dermal fibroblasts with a P199 synthetic polypeptide, which is used in cosmetology for the initiation of antiaging and regenerative effects in the skin, were received. After microdissection, recovery of the spheroids structure with a few surface layers of flattened imbricated arranged cells and polygonal cells of the inner zone in the presence of P199 peptide was faster than in the control group, and was completed within 7 days, presumably due to the remodeling of the survived cells. 相似文献
584.
How does feedback from phage infections influence the evolution of phase variation in Campylobacter?
Campylobacter jejuni (C. jejuni) causes gastroenteritis following the consumption of contaminated poultry meat, resulting in a large health and economic burden worldwide. Phage therapy is a promising technique for eradicating C. jejuni from poultry flocks and chicken carcasses. However, C. jejuni can resist infections by some phages through stochastic, phase-variable ON/OFF switching of the phage receptors mediated by simple sequence repeats (SSR). While selection strength and exposure time influence the evolution of SSR-mediated phase variation (PV), phages offer a more complex evolutionary environment as phage replication depends on having a permissive host organism. Here, we build and explore several continuous culture bacteria-phage computational models, each analysing different phase-variable scenarios calibrated to the experimental SSR rates of C. jejuni loci and replication parameters for the F336 phage. We simulate the evolution of PV rates via the adaptive dynamics framework for varying levels of selective pressures that act on the phage-resistant state. Our results indicate that growth reducing counter-selection on a single PV locus results in the stable maintenance of the phage, while compensatory selection between bacterial states affects the evolutionary stable mutation rates (i.e. very high and very low mutation rates are evolutionarily disadvantageous), whereas, in the absence of either selective pressure the evolution of PV rates results in mutation rates below the basal values. Contrastingly, a biologically-relevant model with two phase-variable loci resulted in phage extinction and locking of the bacteria into a phage-resistant state suggesting that another counter-selective pressure is required, instance, the use of a distinct phage whose receptor is an F336-phage-resistant state. We conclude that a delicate balance between counter-selection and phage-attack can result in both the evolution of phase-variable phage receptors and persistence of PV-receptor-specific phage. 相似文献
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L A Piruzian Iu E Morozov A V Limantsev V A Lomonosov V A Dement'ev 《Izvestiia Akademii nauk SSSR. Seriia biologicheskaia》1989,(1):35-41
Complicated ecological conditions, caused by wide and active application of artificial chemicals, makes it necessary to reveal their effect on human and animal organism and take measures for protecting health. The possibility to use advanced computing and information devices in combination with specialized methodical approaches allowed to elaborate and build a model of a system for revealing fine and multi-aspect influence of various factors on organisms of experimental animals. The results obtained allow to consider creation of similar systems a necessary and feasible task. 相似文献
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590.
tRNA(adenine-1-)-methyltransferase (EC 2.1.1.36) was isolated from the extreme thermophile Thermus thermophilus strain HB8. The specific activity of the enzyme is about 50 000 and the yield of activity more than 20%. The method of isolation consists of five steps and is valid for isolation of mg quantities of the enzyme. The purified protein preparation is practically homogeneous in SDS-gel electrophoresis, the position of the protein band corresponds to a molecular weight of 25 000. By gel filtration on Sephadex G-100 the molecular weight of the native protein was found to be 70 000. These data allow to suggest a subunit structure of the enzyme. The enzyme is highly thermostable and is most active at 80 degrees C. The only activity of the enzyme is to methylate A58 in the T psi X loop of tRNA. 相似文献