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11.
Dorota A. Dobrzanska Matthew T. F. Lamaudière Jessica Rollason Lauren Acton Michael Duncan Sharon Compton John Simms Gareth D. Weedall Igor Y. Morozov 《Microbial biotechnology》2020,13(3):669-682
In agriculture, antibiotics are used for the treatment and prevention of livestock disease. Antibiotics perturb the bacterial gut composition but the extent of these changes and potential consequences for animal and human health is still debated. Six calves were housed in a controlled environment. Three animals received an injection of the antibiotic florfenicol (Nuflor), and three received no treatment. Faecal samples were collected at 0, 3 and 7 days, and bacterial communities were profiled to assess the impact of a therapy on the gut microbiota. Phylogenetic analysis (16S-rDNA) established that at day 7, antibiotic-treated microbiota showed a 10-fold increase in facultative anaerobic Escherichia spp, a signature of imbalanced microbiota, dysbiosis. The antibiotic resistome showed a high background of antibiotic resistance genes, which did not significantly change in response to florfenicol. However, the maintenance of Escherichia coli plasmid-encoded quinolone, oqxB and propagation of mcr-2, and colistin resistance genes were observed and confirmed by Sanger sequencing. The microbiota of treated animals was enriched with energy harvesting bacteria, common to obese microbial communities. We propose that antibiotic treatment of healthy animals leads to unbalanced, disease- and obese-related microbiota that promotes growth of E. coli carrying resistance genes on mobile elements, potentially increasing the risk of transmission of antibiotic resistant bacteria to humans. 相似文献
12.
Orlova A. A. Aleskerova L. E. Vasilieva S. G. Morozov A. S. Ismailov A. D. Lobakova E. S. 《Applied Biochemistry and Microbiology》2021,57(7):828-835
Applied Biochemistry and Microbiology - The toxicity of polyethylenimine-based sorbents and their extracts was evaluated, and their effect on the bioluminescence of Photobacterium phosphoreum... 相似文献
13.
Goncharov Alexey I. Levina Inna S. Shliapina Viktoriia L. Morozov Ivan A. Rubtsov Petr M. Zavarzin Igor V. Smirnova Olga V. Shchelkunova Tatiana A. 《Biochemistry. Biokhimii?a》2021,86(11):1446-1460
Biochemistry (Moscow) - Progesterone and its synthetic analogues act on cells through different types of receptors, affecting proliferation and apoptosis. These compounds exert their effect through... 相似文献
14.
Morozov V. N. Mukhin A. N. Kolyvanova M. A. Belousov A. V. Bushmanov Y. A. Grebennikova T. V. Samoylov A. S. 《Biophysics》2021,66(4):589-595
Biophysics - In recent years, members of the Coronaviridae family have caused outbreaks of respiratory diseases (MERS, SARS, and COVID-19). At the same time, the potential of radiation-induced... 相似文献
15.
Belousov A. V. Morozov V. N. Krusanov G. A. Moiseev A. N. Davydov A. S. Shtil A. A. Klimanov V. A. Kolyvanova M. A. Samoylov A. S. 《Biophysics》2020,65(4):541-547
Biophysics - Gold nanoparticles are promising radiosensitizers for proton radiotherapy. However, the physical mechanisms of gold nanoparticles radiosensitization remain unclear. In the present... 相似文献
16.
Alexander Y. Сhurbanov Tatiana M. Karafet Igor V. Morozov Valeriia Yu. Mikhalskaia Marina V. Zytsar Alexander A. Bondar Olga L. Posukh 《PloS one》2016,11(4)
Hearing loss (HL) is one of the most common sensorineural disorders and several dozen genes contribute to its pathogenesis. Establishing a genetic diagnosis of HL is of great importance for clinical evaluation of deaf patients and for estimating recurrence risks for their families. Efforts to identify genes responsible for HL have been challenged by high genetic heterogeneity and different ethnic-specific prevalence of inherited deafness. Here we present the utility of whole exome sequencing (WES) for identifying candidate causal variants for previously unexplained nonsyndromic HL of seven patients from four unrelated Altaian families (the Altai Republic, South Siberia). The WES analysis revealed homozygous missense mutations in three genes associated with HL. Mutation c.2168A>G (SLC26A4) was found in one family, a novel mutation c.1111G>C (OTOF) was revealed in another family, and mutation c.5254G>A (RAI1) was found in two families. Sanger sequencing was applied for screening of identified variants in an ethnically diverse cohort of other patients with HL (n = 116) and in Altaian controls (n = 120). Identified variants were found only in patients of Altaian ethnicity (n = 93). Several lines of evidences support the association of homozygosity for discovered variants c.5254G>A (RAI1), c.1111C>G (OTOF), and c.2168A>G (SLC26A4) with HL in Altaian patients. Local prevalence of identified variants implies possible founder effect in significant number of HL cases in indigenous population of the Altai region. Notably, this is the first reported instance of patients with RAI1 missense mutation whose HL is not accompanied by specific traits typical for Smith-Magenis syndrome. Presumed association of RAI1 gene variant c.5254G>A with isolated HL needs to be proved by further experimental studies. 相似文献
17.
Morozov I. A. Kamenetskikh A. S. Beliaev A. Yu. Scherban M. G. Lemkina L. M. Eroshenko D. V. Korobov V. P. 《Biophysics》2019,64(3):410-415
Biophysics - Abstract—Elastic polyurethanes are flexible materials used in biomedical products. Plasma treatment is a promising method of surface modification. However, external deformation... 相似文献
18.
Physical maps of the cobmtDNA region were constructed and compared between sugar beet Beta vulgarisL. plants with normal fertility and with cytoplasmic male sterility (CMS). A CMS-associated rearrangement did not affect the coding region of coband combined two mtDNA regions which are normally about 150 kb apart. Two point substitutions were found in the 3"-untranslated region of cob. 相似文献
19.
20.
Minna Ruokonen Laura Kvist Tomas Aarvak Juha Markkola Vladimir V. Morozov Ingar J. Øien Eugeny E. Syroechkovsky Jr. Petteri Tolvanen Jaakko Lumme 《Conservation Genetics》2004,5(4):501-512
The lesser white-fronted goose is a sub-Arctic species with a currently fragmented breeding range, which extends from Fennoscandia to easternmost Siberia. The population started to decline at the beginning of the last century and, with a current world population estimate of 25,000 individuals, it is the most threatened of the Palearctic goose species. Of these, only 30–50 pairs breed in Fennoscandia. A fragment of the control region of mtDNA was sequenced from 110 individuals from four breeding, one staging and two wintering areas to study geographic subdivisions and gene flow. Sequences defined 15 mtDNA haplotypes that were assigned to two mtDNA lineages. Both the mtDNA lineages were found from all sampled localities indicating a common ancestry and/or some level of gene flow. Analyses of molecular variance showed significant structuring among populations (
ST 0.220, P < 0.001). The results presented here together with ecological data indicate that the lesser white-fronted goose is fragmented into three distinctive subpopulations, and thus, the conservation status of the species should be reconsidered. 相似文献