全文获取类型
收费全文 | 882篇 |
免费 | 57篇 |
专业分类
939篇 |
出版年
2022年 | 4篇 |
2021年 | 9篇 |
2019年 | 4篇 |
2018年 | 6篇 |
2017年 | 6篇 |
2016年 | 21篇 |
2015年 | 20篇 |
2014年 | 29篇 |
2013年 | 51篇 |
2012年 | 30篇 |
2011年 | 38篇 |
2010年 | 24篇 |
2009年 | 31篇 |
2008年 | 42篇 |
2007年 | 46篇 |
2006年 | 43篇 |
2005年 | 34篇 |
2004年 | 36篇 |
2003年 | 29篇 |
2002年 | 21篇 |
2001年 | 30篇 |
2000年 | 28篇 |
1999年 | 32篇 |
1998年 | 14篇 |
1997年 | 14篇 |
1996年 | 6篇 |
1995年 | 7篇 |
1994年 | 9篇 |
1993年 | 16篇 |
1992年 | 22篇 |
1991年 | 23篇 |
1990年 | 25篇 |
1989年 | 21篇 |
1988年 | 17篇 |
1987年 | 20篇 |
1986年 | 15篇 |
1985年 | 15篇 |
1984年 | 10篇 |
1983年 | 6篇 |
1982年 | 6篇 |
1981年 | 6篇 |
1980年 | 11篇 |
1979年 | 8篇 |
1978年 | 6篇 |
1977年 | 6篇 |
1976年 | 4篇 |
1975年 | 6篇 |
1974年 | 4篇 |
1973年 | 4篇 |
1972年 | 6篇 |
排序方式: 共有939条查询结果,搜索用时 0 毫秒
1.
Toshihiko Umemoto Isamu Namikawa Zensaku Yoshii Hisanori Konishi 《Microbiology and immunology》1982,26(3):191-198
The osmium-dimethyl sulfoxide-osmium method for clear visualization of intracellular structure was used to observe the detailed inner structure of the spherical bodies produced in vitro by a human oral treponeme. Scanning electron microscopy of the cracked spherical body revealed no morphological differences between the outer and inner surfaces of the spherical body membrane, and that multiple folded or somewhat linear main bodies adhere closely to the inner surface. In addition, axial flagella partially free from the main bodies spread widely within the body to make a network, and a number of blebs ranging from approximately 1 μm to 0.2 μm in diameter were located near the terminal or subterminal areas of the main bodies. The origin of the blebs and the mechanism of spherical body formation are discussed. 相似文献
2.
A chaperonin has been purified from a thermophilic bacterium, Thermus thermophilus. It consists of two kinds of proteins with approximate Mr 58,000 and 10,000 and shows a 7-fold rotational symmetry from the top view and a "football"-like shape from the side view under the electron microscopic view. Its weak ATPase activity is inhibited by sulfite and activated by bicarbonate. ATP causes change of its mobility in nondenaturating polyacrylamide gel electrophoresis. The T. thermophilus chaperonin can promote in vitro refolding of several guanidine HCl-denatured enzymes from thermophilic bacteria. At high temperatures above 60 degrees C, where the native enzymes are stable but their spontaneous refoldings upon dilution of guanidine HCl fail, the chaperonin induces productive refolding in an ATP-dependent manner. No or very poor refolding is induced when the chaperonin is added to the solution aged after dilution. An excess amount of the chaperonin is inhibitory for refolding. At middle temperatures (30-50 degrees C), where spontaneous refoldings of the enzymes occur, the chaperonin arrests refolding in the absence of ATP and refolding is induced when ATP is supplemented. At temperatures below 20 degrees C, where spontaneous refoldings also occur, the chaperonin arrests the refolding but ATP does not induce refolding. 相似文献
3.
A 1000-bp fragment of Enterococcus hirae genomic DNA was amplified by the polymerase chain reaction method, using the oligonucleotide primers designed from amino acid sequences of both amino-terminal and a tryptic fragment of the Na(+)-ATPase alpha-subunit in this organism. DNA sequencing of this product revealed that the amino acid sequence of Na(+)-ATPase alpha-subunit is highly homologous to the corresponding sequences of large (alpha) subunits of vacuolar (archaebacterial) type H(+)-ATPases, supporting our proposal [Kakinuma, Y. and Igarashi, K. (1990) FEBS Lett. 271, 97-101] that the Na(+)-ATPase of this organism belongs to the vacuolar-type ATPase. 相似文献
4.
N Iwai Y Yamano S Chaki F Konishi S Bardhan C Tibbetts K Sasaki M Hasegawa Y Matsuda T Inagami 《Biochemical and biophysical research communications》1991,177(1):299-304
The nucleotide and amino acid sequences for rat type I angiotensin II receptor were deduced through molecular cloning and sequence analysis of its complementary DNAs. The rat angiotensin II receptor consists of 359 amino acid residues and has a sequence similar to G protein-coupled receptors. The expression of this receptor gene was detected in the adrenal, liver and kidney by Northern blotting. Sodium deprivation positively modulated the expression of the receptor gene in the adrenal. No detectable change was observed in the expression levels of this receptor gene between spontaneously hypertensive rats and Wistar-Kyoto rats in the tissues examined including the adrenal, brain, kidney and liver. Interestingly the expression of this receptor gene was developmentally regulated. 相似文献
5.
The reaction products of methyl thioglycolate with dynemicin A, dynemicin H and dynemicin S, were isolated by HPLC purification and identified spectroscopically. The major product, dynemicin H (C30H23NO9), was determined to be a C-8 hydrogen analogue of dynemicins L and N in which the enediyne core is aromatized. The minor product, dynemicin S (C33H27No11S), is an adduct of methyl thioglycolate at the C-8 position. By using NADPH instead of methyl thioglycolate, the reaction with dynemicin A also gives the same major product (dynemicin H). The nucleotide-specific cleavage of dynemicin A induced by addition of methyl thioglycolate is remarkably similar to that induced by addition of NADPH, whereas dynemicins H and S show no DNA cleavage activities. The formation of dynemicins H and S provides a rationale for the reductive and nucleophilic activations of dynemicin A. 相似文献
6.
Hydrocortisone-induced enhancement of expression and changes in methylation of pepsinogen genes in stomach mucosa of the developing rat 总被引:1,自引:0,他引:1
7.
A new method is described for the microdetermination of anionic and cationic surfactants. Anionics can be determined by measuring the degree of their inhibition of enzyme activity (inhibition method). On the other hand, cationics are determined by a method utilizing the finding that the original inhibition of a potent inhibitor previously added to a substrate solution is suppressed by the addition of small amount of cationics (suppression method). In this study, the enzyme is acid phosphatase and p-nitrophenyl phosphate is used as substrate. Employing the method described above, 5–50 ppm of alkylbenzene sulfonate (ABS), 10–90 ppm of sodium dodecyl sulfate (SDS) and 5–15 ppm of dodecyl trimethyl ammonium chloride and dodecyl pyridinium chloride can be determined. The procedure is relatively simple and the analysis requires only 4–5 min. 相似文献
8.
S Yamaguchi K Yonebayashi H Konishi S Tuzi A Naito J K Lanyi R Needleman H Sait? 《European journal of biochemistry》2001,268(8):2218-2228
We have examined the (13)C-NMR spectra of [3-(13)C] Ala-labeled bacteriorhodopsin and its mutants by varying a variety of environmental or intrinsic factors such as ionic strength, temperature, pH, truncation of the C-terminal alpha helix, and site-directed mutation at cytoplasmic loops, in order to gain insight into a plausible surface structure arising from the C-terminal alpha helix and loops. It is found that the surface structure can be characterized as a complex stabilized by salt bridges or metal-mediated linkages among charged side chains. The surface complex in bacteriorhodopsin is most pronounced under the conditions of 10 mM NaCl at neutral pH but is destabilized to yield relaxed states when environmental factors are changed to high ionic strength, low pH and higher temperature. These two states were readily distinguished by associated spectral changes, including suppressed (cross polarization-magic angle spinning NMR) or displaced (upfield) (13)C signals from the C-terminal alpha helix, or modified spectral features in the loop region. It is also noteworthy that such spectral changes, when going from the complexed to relaxed states, occur either when the C-terminal alpha helix is deleted or site-directed mutations were introduced at a cytoplasmic loop. These observations clearly emphasize that organization of the cytoplasmic surface complex is important in the stabilization of the three-dimensional structure at ambient temperature, and subsequently plays an essential role in biological functions. 相似文献
9.
Sato M Sano H Iwaki D Kudo K Konishi M Takahashi H Takahashi T Imaizumi H Asai Y Kuroki Y 《Journal of immunology (Baltimore, Md. : 1950)》2003,171(1):417-425
The lung collectin surfactant protein A (SP-A) has been implicated in the regulation of pulmonary host defense and inflammation. Zymosan induces proinflammatory cytokines in immune cells. Toll-like receptor (TLR)2 has been shown to be involved in zymosan-induced signaling. We first investigated the interaction of TLR2 with zymosan. Zymosan cosedimented the soluble form of rTLR2 possessing the putative extracellular domain (sTLR2). sTLR2 directly bound to zymosan with an apparent binding constant of 48 nM. We next examined whether SP-A modulated zymosan-induced cellular responses. SP-A significantly attenuated zymosan-induced TNF-alpha secretion in RAW264.7 cells and alveolar macrophages in a concentration-dependent manner. Although zymosan failed to cosediment SP-A, SP-A significantly reduced zymosan-elicited NF-kappaB activation in TLR2-transfected human embryonic kidney 293 cells. Because we have shown that SP-A binds to sTLR2, we also examined whether SP-A affected the binding of sTLR2 to zymosan. SP-A significantly attenuated the direct binding of sTLR2 to zymosan in a concentration-dependent fashion. From these results, we conclude that 1) TLR2 directly binds zymosan, 2) SP-A can alter zymosan-TLR2 interaction, and 3) SP-A down-regulates TLR2-mediated signaling and TNF-alpha secretion stimulated by zymosan. This study supports an important role of SP-A in controlling pulmonary inflammation caused by microbial pathogens. 相似文献
10.
Two patterns were found in the shifts of absorption peaks inspectra of intact etiolated Pharbitis cotyledons illuminatedat room temperature. One was a well-known pattern, P649C678C683C672,called the "high-intensity illumination pattern" in this study.The other, called the "low-intensity illumination pattern,"was P649C672. (Received June 16, 1976; ) 相似文献