全文获取类型
收费全文 | 6481篇 |
免费 | 375篇 |
国内免费 | 1篇 |
出版年
2022年 | 23篇 |
2021年 | 68篇 |
2020年 | 42篇 |
2019年 | 54篇 |
2018年 | 76篇 |
2017年 | 63篇 |
2016年 | 104篇 |
2015年 | 175篇 |
2014年 | 202篇 |
2013年 | 471篇 |
2012年 | 295篇 |
2011年 | 313篇 |
2010年 | 194篇 |
2009年 | 182篇 |
2008年 | 271篇 |
2007年 | 289篇 |
2006年 | 279篇 |
2005年 | 287篇 |
2004年 | 277篇 |
2003年 | 257篇 |
2002年 | 268篇 |
2001年 | 232篇 |
2000年 | 238篇 |
1999年 | 189篇 |
1998年 | 79篇 |
1997年 | 71篇 |
1996年 | 65篇 |
1995年 | 71篇 |
1994年 | 73篇 |
1993年 | 75篇 |
1992年 | 145篇 |
1991年 | 118篇 |
1990年 | 124篇 |
1989年 | 129篇 |
1988年 | 97篇 |
1987年 | 110篇 |
1986年 | 92篇 |
1985年 | 87篇 |
1984年 | 72篇 |
1983年 | 69篇 |
1982年 | 38篇 |
1981年 | 48篇 |
1980年 | 29篇 |
1979年 | 62篇 |
1978年 | 29篇 |
1977年 | 46篇 |
1976年 | 31篇 |
1975年 | 30篇 |
1974年 | 39篇 |
1973年 | 33篇 |
排序方式: 共有6857条查询结果,搜索用时 250 毫秒
971.
972.
Fluorescence banding patterns of the rat chromosomes 总被引:1,自引:0,他引:1
973.
974.
Yasuko Koumoto Ryuji Tsugeki Tomoo Shimada Hitoshi Mori Maki Kondo Ikuko Hara-Nishimura Mikio Nishimura 《The Plant journal : for cell and molecular biology》1996,10(6):1119-1125
Chaperonin (Cpn) is one of the molecular chaperones. Cpn10 is a co-factor of Cpn60, which regulates Cpn60-mediated protein folding. It is known that Cpn10 is located in mitochondria and chloroplasts in plant cells. The Escherichia coli homologue of Cpn10 is called GroES. A cDNA for the Cpn10 homologue was isolated from Arabidopsis thaliana by functional complementation of the E. coli groES mutant. The cDNA was 647 bp long and encoded a polypeptide of 98 amino acids. The deduced amino acid sequence showed approximately 50% identity to mammalian mitochondrial Cpn10s and 30% identity to GroES. A Northern blot analysis revealed that the mRNA for the Cpn10 homologue was expressed uniformly in various organs and was markedly induced by heat-shock treatment. The Cpn10 homologue was constitutively expressed in transgenic tobaccos. Immunogold and immunoblot analyses following the subcellular fractionation of leaves from transgenic tobaccos revealed that the Cpn10 homologue was localized in mitochondria and accumulated at a high level in transgenic tobaccos. 相似文献
975.
Kanji Takeo Reiko Tanaka Makoto Miyaji Kazuko Nishimura 《FEMS microbiology letters》1995,129(2-3):231-235
Abstract Stationary-phase cells of Cryptococcus neoformans displayed two morphological characteristics: virtually all the cells were unbudded even in the early stationary phase and even when grown in rich media, and average cell size increased from that of exponential-phase cells. DNA contents for small and large stationary-phase cells were determined by quantitative fluorescence microscopy after DNA staining with propidium iodide or DAPI. Small cells contained G, DNA, whereas large unbudded cells had either a G2 or G1 DNA content, indicating that Cr. neoformans can enter into the stationary phase from either the G1 or G2 period. 相似文献
976.
par Masanao Matsui Yôichi Nakatani Yasuki Mori et Zirô Nikuni 《Bioscience, biotechnology, and biochemistry》2013,77(9):647-649
The synthesis of 3-hydroxymethyl-4,5,7-trimethoxy-2-naphthoic acid lactone (II) is described. 相似文献
977.
Kiyoshi Isono Saburo Suzuki Michio Tanaka Takeo Nanbata Kunitoyo Shibuya 《Bioscience, biotechnology, and biochemistry》2013,77(9):1571-1579
5-Carboxyuracil derivatives were shown to react with aqueous sodium bisulfite in mild condition resulting in facile decarboxylation to give corresponding 5-decarboxy-5,6-dihydrouracil-6-sulfonates and uracils in good yield. The former compounds were quantitatively transformed to the latter in alkaline condition. Mechanistic feature of this reaction was discussed, which implied the initial nucleophilic addition of bisulfite across the 5,6-double bond. 5-Carboxycytosine was also shown to react similarly, however, accompanied by hydrolytic deamination. 相似文献
978.
Takashi Misawa Genichiro Tsuji Takeo Takahashi Eiji Ochiai Ken-ichiro Takagi Kyohei Horie Shinji Kakuda Midori Takimoto-Kamimura Masaaki Kurihara Yosuke Demizu 《Bioorganic & medicinal chemistry》2018,26(23-24):6146-6152
Non-secosteroidal VDR ligands without any assymmetric carbon were designed and synthesized based on the structure of the previously reported non-secosteroidal VDR agonist LG190178. The VDR-agonistic activity of all synthesized compounds was evaluated, and 7b emerged as a potent agonist activity with an EC50 value of 9.26?nM. Moreover, a docking simulation analysis was also performed to determine the binding mode of 7b with VDR-LBD. 相似文献
979.
980.
Hisashi Johno Kentaro Yoshimura Yuki Mori Tokuhide Kimura Manabu Niimi Masaki Yamada Tetsuo Tanigawa Jianglin Fan Sen Takeda 《Metabolomics : Official journal of the Metabolomic Society》2018,14(4):38