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61.
Christopher J Larson Jose G Moreno Kenneth J Pienta Steve Gross Madeline Repollet S Mark O'hara Thomas Russell Leon W M M Terstappen 《Cytometry. Part A》2004,62(1):46-53
BACKGROUND: The prescence of circulating tumor cells (CTCs) in the peripheral blood of cancer patients and their frequency has been correlated with disease status. METHODS: In this study, CTCs were characterized by flow cytometry and fluorescence microscopy after immunomagnetic enrichment from 7.5-ml blood samples collected from patients with prostate cancer in evacuated blood-draw tubes that contained an anticoagulant and a preservative. Events were classified as tumor cell candidates if they expressed cytokeratin, lacked CD45, and stained with the nucleic acid dye 4,6-diamidino-2-phenylindole. RESULTS: In the blood of prostate cancer patients, only few of these events were intact cells. Other CTC events appeared as damaged cells or cell fragments by microscopy. By flow cytometry, these events stained variably with 4,6-diamidino-2-phenylindole and frequently expressed the apoptosis-induced, caspase-cleaved cytokeratin 18. Similar patterns of cell disintegration were observed when cells of the prostate line LNCaP were exposed to paclitaxel before spiking the cells into normal blood samples. CONCLUSIONS: The different observed stages of tumor cell degradation or apoptosis varied greatly between patients and were not found in blood of normal donors. Enumeration of CTCs and identification of CTCs undergoing apoptosis may provide relevant information to evaluate the response to therapy in cancer patients. 相似文献
62.
Z. Adámek I. Sukop P. Moreno Rendón J. Kouil 《Zeitschrift fur angewandte Ichthyologie》2003,19(3):165-169
Natural diets of tench (Tinca tinca L.), carp (Cyprinus carpio L.) and bigmouth buffalo (Ictiobus cyprinellus Val.) were studied to determine food competition among them in polyculture stocks of two different densities. Tench diet consisted mainly of zooplankton (43.8%) and bottom sediments (21.2%). In contrast, carp diet consisted mainly of bottom items including plant debris and detritus (68.8%), which dominated over zooplankton (19.1%). In bigmouth buffalo, most food bulk comprised zooplankton (80.7%). Thus, the resulting food competition (i.e. similarity) was most pronounced between tench and carp (60.8%) and between tench and bigmouth buffalo (47.4%). When comparing growth performance of the three cultured species, the ratios between stocking and harvesting size were: in normal stocking density (392 kg ha?1) 1.72, 3.67 and 2.13, and in doubled stocking density (777 kg ha?1) 1.07, 2.33 and 2.16 in tench, carp and bigmouth buffalo, respectively. 相似文献
63.
In all eukaryotes, entry into mitosis from G2 phase is initiated by a complex of the cdc2 kinase and a B-type cyclin. It has now been shown that, in fission yeast, B-type cyclins also activate cdc2 in G1, thus governing cell-cycle commitment, as well as the onset of S phase. In this article, Karim Labib and Sergio Moreno review the evidence that ruml inhibits the kinase activity of cdc2 associated with B-type cyclins and is an important regulator o f G1 progression in fission yeast. 相似文献
64.
Summary Contents of arginine, ornithine, urea and ammonia in wine were monitored during aging process by two flor veil-forming yeast strains. The patterns of yeast growth were different for the two strains studied. Urea excretion and uptake were dependent on yeast strain, so that finished wines showed different concentrations of urea. The effect of limited aeration was also investigated for Saccharomyces cerevisiae var. capensis. The results indicated that aeration promoted yeast growth in veil, thus reducing urea concentration until its exhaustion in wine. 相似文献
65.
Juan S. Jiménez María J. Benítez Carmen G. Lechuga Manuel Collado Josefa González-Nicólas Francisco J. Moreno 《Molecular and cellular biochemistry》1995,152(1):1-6
In order to elucidate the relationship between hypertension and hypertrophy in the production of heat shock proteins, we studied the induction of the HSP72 synthesis by the heart and gracilis muscles of normo (WKY) and hypertensive (SHR) rats subjected to hyperthermia (42°C±0.5 for 15 min). Two age groups were investigated in each strain: young (2 months, with developing cardiac hypertrophy) and old (18 months, with fully developed chronic cardiac hypertrophy). The gracilis muscle never developed hypertrophy, independently of hypertension or aging. 72 kDa inducible protein was determined by Western blot analysis using a specific monoclonal antibody. We also used a commercial standard, loaded on each blot, to quantitate densitometrically the signal.The heart of young SHR responds to heat shock more than their normotensive age-matched control (298.8±24.7% vs 88.3 ±8.5%, p<0.001). This response is not maintained during aging as we did not find any significant difference between normo-and hypertensive old rats after exposure to hyperthermia (43.6±5.3% vs 65.3±10.4%).Unlike the heart, the gracilis muscle shows a basal spontaneous HSP72 synthesis in both the SHR (71.4±10.8%) and WKY (40.6±11.7%) animals. There was a significant increase in HSP72 synthesis in the gracilis muscle of young SHR with respect to their control (186.2±18.7% vs 115.8±9.9%, p<0.02) which was maintained also during aging (171.9±17.3% vs 95.2±10.5%, p<0.01).In conclusion, these data show that hypertension results in an increased synthesis of HSP72 both in cardiac and gracilis muscle in response to heat shock. This abnormal response is attenuated by aging in the heart but not in the gracilis muscle. Thus, the abnormality seems to be independent from hypertrophy and linked to genetic determination of the disease. 相似文献
66.
67.
Recent advances on cyclins, CDKs and CDK inhibitors 总被引:4,自引:0,他引:4
In eukaryotes, cell division is controlled by cyclin-dependent kinases (CDKs). Here we summarize a few new developments on the regulation of the cell cycle by CDK-cyclin complexes. We have focused on three aspects in which there has been recent progress: the structural analysis of these complexes, the phenotypes of mice carrying knockouts of CDK inhibitors and the role of proteolysis in the regulation of the cell cycle. 相似文献
68.
G. Ramírez-Zarzosa F. Gil R. Latorre A. Ortega A. García-Alcaráz E. Abellán J. M. Vázquez O. López-Albors A. Arencibia F. Moreno 《Cell and tissue research》1995,280(2):217-224
Fibre-type differentiation of the lateral musculature has been studied in Sparus aurata (L.) and Dicentrarchus labrax (L.) during larval development. Histochemical and ultrastructural techniques show two presumptive muscle layers and two germinative zones of presumptive myoblasts. At hatching, myotomal muscle consists of a monolayer of thin undifferentiated cells near the skin (first germinative zone) overlying another mono-layer of small diameter fibres extending hypaxially and epaxially away from the transverse septum. Below this, there is a much thicker, deep layer of fibres, generally large in diameter and polygonal in shape. The presumptive myoblasts are located between these two layers of fibres in the second germinative zone. Initially, the superficial and deep muscle fibres show high and low myosin ATPase activity, respectively. Both layers grow by generating new fibres from the two mentioned germinative zones. At the end of larval life, the superficial layer changes its histochemical profile from high to low myosin ATPase activity and, at the same time, intermediate or pink muscle fibres can be observed by oxidative activity (the NADH-TR reaction). Morphometric analysis shows a significant increase in mean fibre diameter during successive ages, as shown by the Student's t-test (hypertrophic growth). Skewness and kurtosis values of fibre diameters point to the generation of a new fibre population from the germinative zones (hyperplastic growth). 相似文献
69.
Dinucleotide repeat polymorphism at the D4S2458 locus close to the PKD2 locus on human chromosome 4q
Miguel Viribay Dolores Tellería Eladio Velasco Felipe Moreno José L. San Millán 《Human genetics》1995,95(5):601-602
A new polymorphic CA repeat sequence was identified within the candidate region fot the autosomal dominant polycystic kidney disease type 2 (PKD2) locus. It should be a useful marker in the localization of this gene. 相似文献
70.
José Moreno Herminia Rodríguez M. Angeles Vargas Joaquín Rivas Miguel G. Guerrero 《Journal of applied phycology》1995,7(1):17-23
Ten strains of filamentous, heterocystous nitrogen-fixing blue-green algae (cyanobacteria) were screened for growth performance
and tolerance to temperature, pH, irradiance and salinity, together with their potential as producers of phycobiliprotein
pigments. Phycobiliproteins typically accounted for about 50% total cell protein, the prevalent type being C-phycocyanin,
followed by alloppycocyanin, with levels of 17 and 11% d.wt, respectively, in some strains of Anabaena and Nostoc. C-phycoerythrin was the major pigment in several Nostoc strains, reaching 10% d.wt. Some strains represent, therefore, excellent sources of one or more phycobiliproteins. All strains
tolerated an irradiance of ca 2000 μmol photon m-2 s-1. Anabaena sp. ATCC 33047 and Nostoc sp. (Albufera) exhibited the widest optimum range of both temperature (30–45 and 25–40 °C) and pH (6.5–9.5 and 6.0–9.0) for
growth, the former also showing significant salt tolerance. In an outdoor open system, productivity of cultures of two phycoerythrin-rich
strains of Nostoc was over 20 g (d.wt) m-2 d-1 during summer. The growth performance of the allophycocyanin-rich Anabaena sp. ATCC 33047 in outdoor semi-continuous culture has been assessed throughout the year. Productivity values under optimized
conditions ranged from 9 (winter) to 24 (summer) g (d.wt) m-2 d-1. 相似文献