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11.
Three monomorphic and four highly polymorphic microsatellites of Nectomys squamipes were isolated and characterised in a sample of 141 specimens from eight different Brazilian localities. These seven microsatellites and four others previously described in this species were tested in seven other nonfocus sigmodontine species. At least three loci were successfully amplified in every species, but none was amplified in all species. All sequenced products in nonfocus species showed (GT)(n) motifs as in N. squamipes. Several loci were amplified in Nectomys rattus and Oligoryzomys nigripes, while absence of PCR products was observed more frequently in Oxymycterus dasythricus and Akodon cursor. Two of three monomorphic loci in N. squamipes were polymorphic in other species. 相似文献
12.
Ricardo Fagundes da Rocha Marcos Roberto de Oliveira Matheus Augusto de Bittencourt Pasquali Michael Éverton Andrades Max William Soares Oliveira Guilherme Antônio Behr José Cláudio Fonseca Moreira 《Cell biochemistry and function》2010,28(3):190-196
Exercise training has been used for treatment/prevention of many cardiovascular diseases, but the mechanisms need to be clarified. Thus, our aim was to compare oxidative stress parameters between rats submitted to a swimming training and sedentary rats (control). Twelve male rats were divided into two groups: control and exercise training. The exercise training had daily 1 h swimming sessions for 8 weeks and a load (5% of its body mass) was placed in rat's tail. Thereafter the animals were killed, aorta and heart were surgically removed and blood was collected. Body mass gain, thiobarbituric acid reactive species (TBARS), carbonyl content, total reactive antioxidant potential (TRAP), total antioxidant reactivity (TAR), superoxide dismutase (SOD) activity and catalase (CAT) activity were evaluted. The trained rats showed a lower body mass gain and no modifications on heart. An increased SOD activity was observed on aorta after the training, but no changes were seen for CAT activity, which led to an increased SOD/CAT ratio. The arterial TBARS was also increased for trained rats. The decrease in TRAP in exercise training was the single modification on plasma. Our findings suggest that the increased SOD activity could play a role in vascular adaptations to exercise training. Copyright © 2010 John Wiley & Sons, Ltd. 相似文献
13.
K S Moore C L Bevins N Tomassini K M Huttner K Sadler J E Moreira J Reynolds M Zasloff 《The journal of histochemistry and cytochemistry》1992,40(3):367-378
We have characterized a novel peptide-containing cell within the gastric mucosa of Xenopus laevis. The cell is a spherical, multinucleated syncytial structure containing a cytoplasmic space filled with dense rice-shaped granules, and is strikingly similar in morphology to the well-studied granular gland of the amphibian skin. Immunohistochemical and immunogold methods were used to demonstrate that several peptides previously isolated from the granular glands of the skin, including the antimicrobial peptides magainin and PGLa (a peptide with amino-terminal glycine and carboxy-terminal leucinamide), are also stored in granules present in these enteric cells. These data demonstrate that this enteric peptide-producing cell is strikingly similar both morphologically and biochemically to the granular gland, previously considered a highly specialized structure of the amphibian integument. This novel gastric mucosal cell, which we have designated a "granular multinucleated cell," is distinct in its morphology and its diversity of stored peptide products from other well-characterized peptide-containing cells in the vertebrate gastrointestinal tract. 相似文献
14.
Proteins from two species of the genusArtocarpus (A. integrifolia L. andA. incisa L.) were compared by ammonium sulphate fractionation, molecular sieve chromatography and SDS-polyacrylamide gel electrophoresis,
with special attention to the lectins. The protein content and hemagglutinating activity were markedly different in the two
seeds. The protein pattern obtained by both molecular sieve chromatography and SDS-polyacrylamide gel electrophoresis were
quite different. The only similarities found were the elution volume of the lectins in the Sephadex G-100 column and the lectin
bands (11 500 and 15 000 daltons) in SDS-polyacrylamide gel electrophoresis. 相似文献
15.
Filipe Pereira Cláudia Moreira Luís Fonseca Barbara van Asch Manuel Mota Isabel Abrantes António Amorim 《PloS one》2013,8(2)
The pinewood nematode, Bursaphelenchus xylophilus, is one of the greatest threats to coniferous forests worldwide, causing severe ecological damage and economic loss. The biology of B. xylophilus is similar to that of its closest relative, B. mucronatus, as both species share food resources and insect vectors, and have very similar morphological characteristics, although little pathogenicity to conifers has been associated with B. mucronatus. Using both nuclear and mitochondrial DNA markers, we show that B. xylophilus and B. mucronatus form distinct phylogenetic groups with contrasting phylogeographic patterns. B. xylophilus presents lower levels of intraspecific diversity than B. mucronatus, as expected for a species that evolved relatively recently through geographical or reproductive isolation. Genetic diversity was particularly low in recently colonised areas, such as in southwestern Europe. By contrast, B. mucronatus displays high levels of genetic diversity and two well-differentiated clades in both mitochondrial and nuclear DNA phylogenies. The lack of correlation between genetic and geographic distances in B. mucronatus suggests intense gene flow among distant regions, a phenomenon that may have remained unnoticed due to the reduced pathogenicity of the species. Overall, our findings suggest that B. xylophilus and B. mucronatus have different demographic histories despite their morphological resemblance and ecological overlap. These results suggest that Bursaphelenchus species are a valuable model for understanding the dispersion of invasive species and the risks posed to native biodiversity and ecosystems. 相似文献
16.
Felipe F.D. Oliveira Diego C.B.D. Santos Alexandre A.M. Lapis José R. Corrêa Alexandre F. Gomes Fabio C. Gozzo Paulo F. Moreira Virgínia C. de Oliveira Frank H. Quina Brenno A.D. Neto 《Bioorganic & medicinal chemistry letters》2010,20(20):6001-6007
Newly designed 2,1,3-benzothiadiazole-containing fluorescent probes with four excited state intramolecular proton transfer (ESIPT) sites were successfully tested in live cell-imaging assays using a confluent monolayer of human stem-cells (tissue). All tested dyes were compared with the commercially available DAPI and gave far better results. 相似文献
17.
Nunney L Yuan X Bromley R Hartung J Montero-Astúa M Moreira L Ortiz B Stouthamer R 《PloS one》2010,5(11):e15488
Invasive diseases present an increasing problem worldwide; however, genomic techniques are now available to investigate the timing and geographical origin of such introductions. We employed genomic techniques to demonstrate that the bacterial pathogen causing Pierce's disease of grapevine (PD) is not native to the US as previously assumed, but descended from a single genotype introduced from Central America. PD has posed a serious threat to the US wine industry ever since its first outbreak in Anaheim, California in the 1880s and continues to inhibit grape cultivation in a large area of the country. It is caused by infection of xylem vessels by the bacterium Xylella fastidiosa subsp. fastidiosa, a genetically distinct subspecies at least 15,000 years old. We present five independent kinds of evidence that strongly support our invasion hypothesis: 1) a genome-wide lack of genetic variability in X. fastidiosa subsp. fastidiosa found in the US, consistent with a recent common ancestor; 2) evidence for historical allopatry of the North American subspecies X. fastidiosa subsp. multiplex and X. fastidiosa subsp. fastidiosa; 3) evidence that X. fastidiosa subsp. fastidiosa evolved in a more tropical climate than X. fastidiosa subsp. multiplex; 4) much greater genetic variability in the proposed source population in Central America, variation within which the US genotypes are phylogenetically nested; and 5) the circumstantial evidence of importation of known hosts (coffee plants) from Central America directly into southern California just prior to the first known outbreak of the disease. The lack of genetic variation in X. fastidiosa subsp. fastidiosa in the US suggests that preventing additional introductions is important since new genetic variation may undermine PD control measures, or may lead to infection of other crop plants through the creation of novel genotypes via inter-subspecific recombination. In general, geographically mixing of previously isolated subspecies should be avoided. 相似文献
18.
Luiz Henrique Soares de Andrade Wilson Max Almeida Monteiro de Moraes Eduardo Hiroshi Matsuo Junior Elizabeth de Orleans Carvalho de Moura Hanna Karen Moreira Antunes Jairo Montemor Ednei Luiz Antonio Danilo Sales Bocalini Andrey Jorge Serra Paulo José Ferreira Tucci Patricia Chakur Brum Alessandra Medeiros 《Molecular and cellular biochemistry》2015,402(1-2):193-202
19.
A. I. Rodarte-Morales G. Feijoo M. T. Moreira J. M. Lema 《World journal of microbiology & biotechnology》2011,27(8):1839-1846
Today, more than 3,000 pharmaceutical and personal care products (PPCPs) are used and released into the environment at low
doses but they are barely degraded in wastewater treatment plants. One of the potential alternatives to effectively degrade
PPCPs is based on the use of white-rot fungi (WRF) and involves the oxidative action of extracellular fungal enzymes. The
aim of this work is to study the potential ability of three WRF strains, an anamorph species of Bjerkandera sp. R1, Bjerkandera adusta and Phanerochaete chrysosporium, to degrade PPCPs belonging to different therapeutic groups: anti-depressants (citalopram and fluoxetine), antibiotics (sulfamethoxazole),
anti-inflammatory drugs (diclofenac, ibuprofen and naproxen), anti-epileptics (carbamazepine), tranquilizers (diazepam) and
fragrances (celestolide, galaxolide and tonalide). The results reported complete degradation of all the PPCPs except for fluoxetine
and diazepam, which were partially removed in percentages from 23 to 57%. In the case of fragrances, these compounds were
neither detected in the fungal cultures nor in the abiotic controls, indicating the possibility of volatilization during the
experiment. 相似文献
20.