全文获取类型
收费全文 | 47800篇 |
免费 | 3898篇 |
国内免费 | 5篇 |
专业分类
51703篇 |
出版年
2023年 | 231篇 |
2021年 | 328篇 |
2020年 | 298篇 |
2019年 | 285篇 |
2018年 | 923篇 |
2017年 | 954篇 |
2016年 | 1028篇 |
2015年 | 939篇 |
2014年 | 1125篇 |
2013年 | 1935篇 |
2012年 | 3224篇 |
2011年 | 3607篇 |
2010年 | 1856篇 |
2009年 | 1190篇 |
2008年 | 2947篇 |
2007年 | 3006篇 |
2006年 | 2805篇 |
2005年 | 2511篇 |
2004年 | 2414篇 |
2003年 | 2254篇 |
2002年 | 2251篇 |
2001年 | 1617篇 |
2000年 | 1893篇 |
1999年 | 1015篇 |
1998年 | 463篇 |
1997年 | 357篇 |
1996年 | 436篇 |
1995年 | 370篇 |
1994年 | 405篇 |
1993年 | 359篇 |
1992年 | 448篇 |
1991年 | 397篇 |
1990年 | 362篇 |
1989年 | 309篇 |
1988年 | 326篇 |
1987年 | 305篇 |
1986年 | 268篇 |
1985年 | 367篇 |
1984年 | 423篇 |
1983年 | 369篇 |
1982年 | 343篇 |
1981年 | 327篇 |
1980年 | 278篇 |
1979年 | 304篇 |
1978年 | 288篇 |
1977年 | 283篇 |
1976年 | 261篇 |
1975年 | 316篇 |
1974年 | 240篇 |
1973年 | 235篇 |
排序方式: 共有10000条查询结果,搜索用时 15 毫秒
81.
W. Schäfer 《Helgoland Marine Research》1985,39(3):341-356
Early developmental stages of theAnemonia germ are characterized by asynchronously dividing nuclei and an extreme delay of blastomere differentiation. The nuclei migrate to the periphery, whereas nutritive substances remain in the interior. Following this stage, the appearance of cell boundaries results in the formation of the blastoderm and the simultaneous division of the yolk into many fragments. Most of them are exclusively filled with reserve material; only very few contain zooxanthellae or nuclei. On the embryo's surface, conically shaped bundles of long microvilli are obvious. They appear to be less regularly arranged than the spines of oocytes before insemination. Pigment granules that have originated from fusing Golgi vesicles are crowded peripherally in the blastoderm cells. In the nucleoplasm single annulate lamellae that seem to be cut off from the nuclear envelope can frequently be found. There is no further cellular differentiation until gastrulation is completed. Though yolk-containing cellular fragments occupy nearly the whole blastocoel, entoderm formation occurs by invagination. Ultrastructural observations provide evidence of the existence of interstices between entoderm cells that allow all nutritive substances to pass gradually into the gastric cavity. In the region of the blastoporus there are cellular processes enveloping reserve material. Presumably, these observations indicate a so-called filtration of nutritive yolk (Korschelt & Heider, 1909) that might represent an additional mode for the transfer of yolk-containing cellular fragments from blastocoel into gastrocoel. 相似文献
82.
83.
84.
85.
Walter Fahn Eva Laußermair Brigitte Deus-Neumann Joachim Stöckigt 《Plant cell reports》1985,4(6):337-340
From differentiated plants of Catharanthus roseus (L.) G. Don we have isolated a specific enzyme of the vindoline biosynthetic pathway catalysing the S-adenosylmethionine-dependent methylation of 11-O-demethyl-17-O-deacetyl-vindoline. The enzyme we named S-adenosyl-L-methionine : 11-O-demethyl-17-O-deacetylvindoline 11-O-methyltransferase. This transferase exhibits a high substrate specificity. Obviously the O-methylation at C-11 precedes the O-acetylation at the C-17 position during the biosynthesis of vindoline.A second enzyme was detected which hydrolyses the acetyl function of vindoline. The distribution of this acetylesterase in C. roseus plants demonstrates that the enzyme is not specifically associated with the vindoline distribution in the plant material. Most probably this enzyme plays no essential role in the biosynthesis of vindoline. 相似文献
86.
Walter Fahn Heidrun Gundlach Brigitte Deus-Neumann Joachim Stöckigt 《Plant cell reports》1985,4(6):333-336
From differentiated plants of Catharanthus roseus (L.) G. Don, a specific enzyme was isolated and named acetyl-CoA : 17-O-deacetylvindoline 17-O-acetyltransferase, acting on the biosynthetic formation of the Aspidosperma type alkaloid vindoline.The enzyme shows a high selectivity towards different substrates. The acetyl-CoA-dependent transferase also catalyses the reverse reaction by hydrolysis of the 17-O-acetyl group of vindoline in the presence of free CoA. This enzyme is localized only in vindoline-containing plant parts, but was so far not detectable in cell suspension cultures of C. roseus. The enzyme allows the synthesis of labelled vindoline with high specific activity, applicable for instance as tracer for radioimmunoassays of vindoline. 相似文献
87.
Primary production data from the south-eastern Weddell Sea 总被引:2,自引:2,他引:0
Klaus von Bröckel 《Polar Biology》1985,4(2):75-80
Summary Phytoplankton production for three size classes (<20 m, 20–100 m, >100 m), total primary production and qualitative composition of phytoplankton populations were recorded from 18 stations in the south-eastern Weddell Sea in February/March 1983. Total primary production ranged between 80 and 1670 mg C m-2 d-1 with an average of 670 mg C m-2 d-1, nearly 70% of which was contributed by the <20 m size fraction (usually pennate and/or centric diatoms). Production of phytoplankton was in the higher range of values reported by other authors for the same region. Variations in primary production could not be attributed to composition of populations, ambient light levels or concentrations of macronutrients (N, P, Si). Phytoplankton populations had a higher diversity in the deeper parts of the Weddell Sea and coincided with different oceanographic situations. Three zones (along the shelf-ice edge from Atka Bay to Halley Bay, west of Halley Bay and off the Filchner/Rønne Ice Shelf) with different communities could be clearly distinguished. 相似文献
88.
Jan Dahmén Torbjörn Frejd Göran Magnusson Ghazi Noori Anne-Sofie Carlström 《Carbohydrate research》1984,125(2):237-245
The applicability of 2-bromoethyl glycosides in carbohydrate synthesis is demonstrated by the synthesis of glycosides of alpha-L-Fuc-(1----2)-D-Gal and beta-D-Gal-(1----4)-D-GlcNAc. The bromoethyl aglycon was transformed into the methoxycarbonylethylthioethyl spacer, which allowed coupling of the sugars to proteins (BSA and KLH). 相似文献
89.
Lipid/myelin basic protein multilayers. A model for the cytoplasmic space in central nervous system myelin 总被引:4,自引:0,他引:4
A multilayered complex forms when a solution of myelin basic protein is added to single-bilayer vesicles formed by sonicating myelin lipids. Vesicles and multilayers have been studied by electron microscopy, biochemical analysis, and X-ray diffraction. Freeze-fracture electron microscopy shows well-separated vesicles before myelin basic protein is added, but afterward there are aggregated, possibly multilayered, vesicles and extensive planar multilayers. The vesicles aggregate and fuse within seconds after the protein is added, and the multilayers form within minutes. No intra-bilayer particles are seen, with or without the protein. Some myelin basic protein, but no lipid, remains in the supernatant after the protein is added and the complex sedimented for X-ray diffraction. A rather variable proportion of the protein is bound. X-ray diffraction patterns show that the vesicles are stable in the absence of myelin basic protein, even under high g-forces. After the protein is added, however, lipid/myelin basic protein multilayers predominate over single-bilayer vesicles. The protein is in every space between lipid bilayers. Thus the vesicles are torn open by strong interaction with myelin basic protein. The inter-bilayer spaces in the multilayers are comparable to the cytoplasmic spaces in central nervous system myelins . The diffraction indicates the same lipid bilayer thickness in vesicles and multilayers, to within 1 A. By comparing electron-density profiles of vesicles and multilayers, most of the myelin basic protein is located in the inter-bilayer space while up to one-third may be inserted between lipid headgroups. When cytochrome c is added in place of myelin basic protein, multilayers also form. In this case the protein is located entirely outside the unchanged bilayer. Comparison of the various profiles emphasizes the close and extensive apposition of myelin basic protein to the lipid bilayer. Numerous bonds may form between myelin basic protein and lipids. Cholesterol may enhance binding by opening gaps between diacyl-lipid headgroups. 相似文献
90.
Isaksson Axel Philips Anna–Carin Göransson Eva Björkenfeldt Henrik 《Acta veterinaria Scandinavica》1987,28(3-4):455-457
The electrical conductivity (EC) of milk is mainly a function of the electrolyte concentration in the milk and therefore raised in mastitis. The present investigation was aimed at elaborating, if possible, a diagnostic model for screening purposes based on EC determinations and consistent with the diagnostic procedures and interpretations commonly used in laboratory milk diagnosis in the Nordic countries (Klastrup 1975). According to this diagnosis (here called reference diagnosis) cell numbers above 300,000/ml (cell count or the corresponding CMT-score) in foremilk quarter samples during the main part of the lactation period and significantly above the lowest value on within-udder comparison during late lactation are considered indicative of mastitis and bacteriological examinations are made when called for. 相似文献