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961.
962.
Human-driven landscape changes may promote plant invasions by increasing propagule pressure and providing favourable conditions for non-native species to establish and spread. The increase in invasion levels might not be immediate but rather exhibit a time-lag (i.e. invasion debt). Moreover, the relationship between invasion and landscape history (i.e. changes in landscape composition) might be extremely complex as it might also include extinction debts of invasive species currently in regression. In order to understand the effect of landscape history on plant invasion, we studied the invasion level in coastal plant communities affected by intense human-driven landscape alteration in the last 50 years. We identified all non-native plant species within 809 grid cells (250 × 250 m) along ~500 km of the Spanish SW coast. We tested the association of (1) non-native richness conditional on the overall presence of non-natives (at least one species), and (2) the occurrence of the most frequent non-native species, with the percentage of human land-cover categories in 1956, 1991 and 2007 using a multimodel inference approach. We used these models to project future invasion patterns in the region. We found non-native richness to be more associated with land-cover variables in 1956 than in 2007, suggesting the existence of an invasion debt, but not for the overall presence of non-natives. For most frequent species, the effects of past landscape alteration depended on the introduction pathway and the region of origin. Species used in agriculture were more related to past cropland area, while most of the species used in gardening or forestry showed higher affinity for recently altered areas. These results highlight the relevance of the species’ introduction history and landscape history in assessing future long-term invasion trends.  相似文献   
963.
Introduction: The application of new proteomics methods may help to identify new diagnostic/predictive molecular markers in an attempt to improve the clinical management of atherosclerosis.

Areas covered: Technological advances in proteomics have enhanced its sensitivity and multiplexing capacity, as well as the possibility of studying protein interactions and tissue structure. These advances will help us better understand the molecular mechanisms at play in atherosclerosis as a biological system. Moreover, this should help identify new predictive/diagnostic biomarkers and therapeutic targets that may facilitate effective risk stratification and early diagnosis, with the ensuing rapid implementation of treatment. This review provides a comprehensive overview of the novel methods in proteomics, including state-of-the-art techniques, novel biological samples and applications for the study of atherosclerosis.

Expert commentary: Collaboration between clinicians and researchers is crucial to further validate and introduce new molecular markers to manage atherosclerosis that are identified using the most up to date proteomic approaches.  相似文献   

964.
Biotic and abiotic particles shape the microspatial architecture that defines the microbial aquatic habitat, being particles highly variable in size and quality along oceanic horizontal and vertical gradients. We analysed the prokaryotic (bacterial and archaeal) diversity and community composition present in six distinct particle size classes ranging from the pico‐ to the microscale (0.2 to 200 μm). Further, we studied their variations along oceanographic horizontal (from the coast to open oceanic waters) and vertical (from the ocean surface into the meso‐ and bathypelagic ocean) gradients. In general, prokaryotic community composition was more variable with depth than in the transition from the coast to the open ocean. Comparing the six size‐fractions, distinct prokaryotic communities were detected in each size‐fraction, and whereas bacteria were more diverse in the larger size‐fractions, archaea were more diverse in the smaller size‐fractions. Comparison of prokaryotic community composition among particle size‐fractions showed that most, but not all, taxonomic groups have a preference for a certain size‐fraction sustained with depth. Species sorting, or the presence of diverse ecotypes with distinct size‐fraction preferences, may explain why this trend is not conserved in all taxa.  相似文献   
965.
A rapid and sensitive non-radioactive Northern blot protocol is described which has been optimised in several critical steps. This is based on a formaldehyde-denaturing agarose gel electrophoresis, an alkaline transfer, hybridisation with digoxigenin-DNA probes and detection with a chemiluminescent substrate. This method allows even low abundance mRNAs to be detected in total RNA samples from mammalian tissues.  相似文献   
966.
The fungus Pleurotus ostreatus was investigated to assess its ability to remove diclofenac, ketoprofen, and atenolol spiked at 10 mg/L each one in hospital wastewater. The degradation test was carried out in a fluidized bed bioreactor testing both the batch and the continuous mode (hydraulic retention time in the range 1.63–3 days). In batch mode, diclofenac disappeared in less than 24 h, ketoprofen was degraded up to almost 50% in 5 days while atenolol was not removed. In continuous mode, diclofenac and ketoprofen removals were about 100% and 70% respectively; atenolol degradation was negligible during the first 20 days but it increased up to 60% after a peak of laccase production and notable biomass growth. In order to identify the enzymatic system involved, further experiments were carried out in flasks. Purified laccase completely transformed atenolol and diclofenac in less than 5 h, but not ketoprofen. In vivo experiments suggested that cytochrome P450 could be involved in diclofenac and ketoprofen degradation, while partial correlation studies confirmed the role of laccase in atenolol and diclofenac degradation. Two intermediates of diclofenac and ketoprofen were detected by nuclear magnetic resonance. Moreover P. ostreatus was able to reduce chemical oxygen demand of the hospital wastewater which is an important advantage comparing to other fungi in order to develop a wastewater treatment process. © 2017 American Institute of Chemical Engineers Biotechnol. Prog., 33:1529–1537, 2017  相似文献   
967.
Conservation Implications of Invasion by Plant Hybridization   总被引:12,自引:0,他引:12  
The increasing number of invasive exotic plant species in many regions and the continuing alteration of natural ecosystems by humans promote hybridization between previously allopatric species; among both native as well as between native and introduced species. We review the ecological factors and mechanisms that promote such hybridization events and their negative consequences on biological diversity. Plant invasions through hybridization may occur in four different ways: hybridization between native species, hybridization between an exotic species and a native congener, hybridization between two exotics and by the introduction and subsequent spread of hybrids. The main harmful genetic effect of such hybrids on native species is the loss of both genetic diversity and of locally adapted populations, such as rare and threatened species. The spread of aggressive hybrid taxa can reduce the growth of, or replace, native species. The main factor promoting the formation of hybrids is species dispersal promoted by humans. However, the success and spread of hybrids is increased by disturbance and fragmentation of habitats, thus overcoming natural crossing barriers, and range expansions due to human activity. There are differences in flowering, pollination and seed dispersal patterns between parental species and hybrids. Hybrid resistance to pathogens and herbivores may also enhance the success of hybrids. To predict the mechanisms and consequences of invasions mediated by hybridization, extensive data on hybrid ecology and biology are needed, as well as carefully designed field experiments focused on the comparative ecology of parental populations and hybrids.  相似文献   
968.
Abstract. This study explored the validity of three responses of vegetation to increased soil erosion: reduction of vegetation cover, number of species and reduced substitution of species. 201 relevés, including edaphic and geomorphological data, were surveyed in the intensely eroded Eocene marls of the Prepyrenees (NE Spain). Changes in plant species’ presence in relevés from different degradation stages were compared. The level of vegetation degradation was defined as the total phanerogam cover which, in the studied area, was correlated to the degree of soil erosion. The considered trends were validated. Reduction of phanerogam cover and species number were gradual from low to high‐eroded areas. Vegetation degradation explained 48% of the species number variance. In the later stages of degradation a significant substitution of species was not observed, only a lower frequency of occurrence of several species that appeared in the whole set of relevés. Through the process of degradation, 47% of species displayed significantly reduced frequencies as degradation increased, none showed a significant increase in frequency. It is concluded that there are no characteristic species in these plant communities that survive in the severely eroded marls. Among the few species that had increased in frequency, most only colonised favourable micro‐environments.  相似文献   
969.
During the last century, otter populations in the Mediterranean area of the Iberian Peninsula were dramatically reduced and disappeared in many localities. A reintroduction programme was established in north-eastern Spain (Muga and Fluvià basins and the “Aiguamolls de l’Empordà” wetlands), by releasing 42 otters from four different Iberian populations, between 1995 and 2002. In order to evaluate the success of the reintroduction programme, we investigated microsatellite variation in the native populations of released otters as well as in the population present in the release area in 2004. We used non-invasively collected samples as the DNA source to avoid disturbing the animals in the wild. Laboratory procedures included the screening of samples and a multiple-tubes approach to detect and correct genotyping errors. Our results show that founders have been replaced by descendants in the release area and the population is in Hardy-Weinberg equilibrium, with a 1:1 sex ratio. Western Iberian otters—representing two-thirds of the founder group and released earlier—have greatly contributed to the genetic composition of the current population. The genetic patterns of otters found in a basin north of the studied area suggest a common origin with the released population. We also detected in a few otters the presence of alleles not identified in the original founder group, and we shall discuss the possible origin of these alleles. This paper is dedicated to the memory of Dr. Xavier Domingo-Roura.  相似文献   
970.
Double strand breaks (DSB) are critical lesions involved in the formation of chromosomal aberrations. In response to DNA damage, the cell has mechanisms of repair and cell-cycle control to maintain the genome integrity in which BRCA1 gene is implicated. In the present study an evaluation of the radio-induced damage in G(2) phase of the cell cycle in lymphocytes from BRCA1 heterozygotes is presented. For this purpose Calyculin-A-based premature chromosome condensation (PCC) combined with mitotic arrest has been applied to examine with conventional cytogenetics the damage in G(2) and M phase cells, and to evaluate the G(2)-to-M phase transition. Irradiated peripheral blood lymphocytes from seven heterozygote females (BRCA1(+/-)) and seven control females (BRCA1(+/+)) have been analyzed. The mean proportion of G(2) cells in BRCA1(+/-) was significantly higher than in BRCA1(+/+), indicating a higher G(2) delay after IR exposure in cells from BRCA1(+/-) females. On the other hand, whereas the mean frequency of chromatid breaks (chtb) in G(2) cells was not statistically different between both groups, the mean frequency of chtb in M cells of the BRCA1(+/-) group was significantly higher than in the BRCA1(+/+) one. Moreover, the mean proportion of M cells with aberrations was significantly higher in BRCA1(+/-) than in BRCA1(+/+) suggesting that in spite of the higher G(2) delay of BRCA1(+/-) more damaged cells are able to pass the G(2)-to-M transition.  相似文献   
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