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631.
Angel Montoya Maria J. Gómez-Lechón Jose V. Castell 《In vitro cellular & developmental biology. Plant》1989,25(4):358-364
Summary Supplementation of Ham's F12 culture medium with essential amino acids (EAA) up to the rat plasma levels increased the rates
of synthesis of albumin and transferrin by cultured rat hepatocytes by 1.3 and 1.7, respectively. Fifty percent of this increase
could be attributed to three of the EAA: the branched-chain amino acids (BCAA: Leu Ile and Val). Non-branched-chain essential
amino acids (non-BC-EAA) stimulated only 25% of the increase produced by the whole EAA mixture. When each EAA was tested individually,
none of them caused an appreciable increase in albumin and transferrin in culture medium. When the concentrations of all EAA
were raised to rat postprandial portal levels, albumin and transferrin synthesis rates reached a maximum, increasing by 3.2
and 3.5, respectively. Supplementation with BCAA at postprandial portal concentrations increased albumin and transferrin synthesis
rates by 2.2 and 2.0, respectively, and had no noteworthy effect on the synthesis of cellular proteins. Non-BC-EAA at their
postprandial portal concentrations increased albumin and transferrin synthesis rates by 1.7 and 1.9, respectively. Supplementation
with alanine to reach a nitrogen content equal to that of the modified EAA-enriched medium had no stimulatory effect. Our
results show that EAA have a specific effect on the synthesis of plasma proteins by cultured hepatocytes, and that BCAA at
physiologic concentrations account for the major part of this stimulatory effect. Consequently, EAA and particularly BCAA
concentration should be elevated in serum-free nutrient media to sustain maximum plasma protein synthesis. 相似文献
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Colby Stotesbury Eric B. Wong Lingjuan Tang Brian Montoya Cory J. Knudson Carolina R. Melo‐Silva Luis J. Sigal 《Aging cell》2020,19(7)
It is known that aging decreases natural resistance to viral diseases due to dysfunctional innate and adaptive immune responses, but the nature of these dysfunctions, particularly in regard to innate immunity, is not well understood. We have previously shown that C57BL/6J (B6) mice lose their natural resistance to footpad infection with ectromelia virus (ECTV) due to impaired maturation and recruitment of natural killer (NK) cells to the draining popliteal lymph node (dLN). More recently, we have also shown that in young B6 mice infected with ECTV, the recruitment of NK cells is dependent on a complex cascade whereby migratory dendritic cells (mDCs) traffic from the skin to the dLN, where they produce CCL2 and CCL7 to recruit inflammatory monocytes (iMOs). In the dLN, mDCs also upregulate NKG2D ligands to induce interferon gamma (IFN‐γ) expression by group 1 innate lymphoid cells (G1‐ILCs), mostly NK in cells but also some ILC1. In response to the IFN‐γ, the incoming uninfected iMOs secret CXCL9 to recruit the critical NK cells. Here, we show that in aged B6 mice, the trafficking of mDCs to the dLN in response to ECTV is decreased, resulting in impaired IFN‐γ expression by G1‐ILCs, reduced accumulation of iMOs, and attenuated CXCL9 production by iMOs, which likely contributes to decrease in NK cell recruitment. Together, these data indicate that defects in the mDC response to viral infection during aging result in a reduced innate immune response in the dLN and contribute to increased susceptibility to viral disease in the aged. 相似文献
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Roya Yousefi Eugenio F Fornasiero Lukas Cyganek Julio Montoya Stefan Jakobs Silvio O Rizzoli Peter Rehling David PacheuGrau 《EMBO reports》2021,22(4)
Mitochondria possess a small genome that codes for core subunits of the oxidative phosphorylation system and whose expression is essential for energy production. Information on the regulation and spatial organization of mitochondrial gene expression in the cellular context has been difficult to obtain. Here we devise an imaging approach to analyze mitochondrial translation within the context of single cells, by following the incorporation of clickable non‐canonical amino acids. We apply this method to multiple cell types, including specialized cells such as cardiomyocytes and neurons, and monitor with spatial resolution mitochondrial translation in axons and dendrites. We also show that translation imaging allows to monitor mitochondrial protein expression in patient fibroblasts. Approaching mitochondrial translation with click chemistry opens new avenues to understand how mitochondrial biogenesis is integrated into the cellular context and can be used to assess mitochondrial gene expression in mitochondrial diseases. 相似文献
637.
Knowledge Integration and Good Marine Governance: A Multidisciplinary Analysis and Critical Synopsis
Poto Margherita Paola Kuhn Annegret Tsiouvalas Apostolos Hodgson Kara K. Treffenfeldt Montoya Valentina M.Beitl Christine 《Human ecology: an interdisciplinary journal》2022,50(1):125-139
Human Ecology - Our research addresses knowledge integration for the good governance of the environment and the oceans: (a) through a comprehensive legal, political science, and anthropological... 相似文献
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