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G. De Benedictis E. Falcone G. Rose R. Ruffolo P. Spadafora G. Baggio S. Bertolini D. Mari R. Mattace D. Monti M. Morellini P. Sansoni C. Franceschi 《Human genetics》1997,99(3):312-318
To identify possible genetic factors affecting human longevity we compared allele pools at two candidate loci for longevity
between a sample of 143 centenarians (S) and a control sample of 158 individuals (C). The candidate loci were APOB and TPO,
which code for apolipoprotein B and thyroid peroxidase, respectively. Both restriction fragment length (RFL) (XbaI2488 and EcoRI4154) and variable number of tandem repeat (VNTR) (3′APOB-VNTR) polymorphisms were analysed at the APOB locus; the TPO-VNTR polymorphism
(intron 10) was analysed at the TPO locus. The main result of the investigation was that there is an association between the
APOB locus and longevity that is revealed only when multiallelic polymorphisms are considered. In particular: (i) the frequency
of 3′APOB-VNTR alleles with fewer than 35 repeats is significantly lower in cases than in controls; (ii) the linkage disequilibrium
between the XbaI-RFLP and the EcoRI-RFLP is significantly different from 0 in cases but not in controls; (iii) the EcoRI-RFLP and XbaI-RFLP allele frequencies do not discriminate between cases and controls. The differences observed between case and control
allele pools are specific to the APOB locus, since no significant difference was observed at the TPO locus.
Received: 27 November 1995 / Revised: 24 July 1996 相似文献
114.
Sylvie Baltora-Rosset Danielle Marty François-Yves Dupradeau Emmanuel Pauthe Véronique Larreta-Garde Gilles Gacel Jean-Pierre Monti 《International journal of peptide research and therapeutics》1997,4(4-6):391-395
Summary The conformations of thermolysin synthetic substrates in H2O/D2O (9/1) and glycerol-d
5 (5 M) are investigated using two-dimensional nuclear magnetic resonance spectroscopy and molecular modeling. The structures
obtained from molecular modeling and NMR studies are compared. Comparisons of these structures with bound inhibitor in the
active site of thermolysin are also discussed. 相似文献
115.
François-Yves Dupradeau Guillaume Le Flem Jean-Michel Wieruszeski Manuel Dauchez Alain Alix Véronique Larreta-Garde Jean-Pierre Monti 《International journal of peptide research and therapeutics》1997,4(4-6):489-495
Summary
1H-NMR studies of the bovine insulin S-sulfonated B-chain are reported in H2O/D2O (9/1) and in glycerol-d
5 (5 M) using two-dimensional NMR spectroscopy. The first results show that the oxidized insulin B-chain secondary structure
differs from that of native insulin by a loss of the α-helix between the two disulfide bridges and that the glycerol favours
the structuring of the peptide. 相似文献
116.
M G Battelli S Musiani B Monti L Buonamici M Sparapani A Contestabile F Stirpe 《Neurochemistry international》2001,39(2):83-93
Microglial cells, like macrophages, are very sensitive to ricin, a galactose-specific toxic lectin belonging to the family of ribosome-inactivating proteins. This toxin can be taken up by most cells through the binding of its B chain to galactose-containing molecules on the cell membrane. In macrophagic cell types it can be internalised also by mannose receptors which are present on the surface of these cells. Endocytosis of the toxin by either pathway was evaluated by ricin toxicity to primary cultures of rat microglial cells and to a microglial N11 cell line in the presence or absence of lactose and mannan, which compete for the endocytosis via the ricin lectin chain or cellular mannose receptors, respectively. Results were compared with those obtained in cultures of mouse macrophages, human monocytes, and a monocytic JM cell line. All cultures were protected from ricin toxicity more by lactose than by mannan, indicating that ricin endocytosis via its lectin B chain is prevalent over that mediated by cellular mannose receptors. However, a partial protection by mannan was observed in all cases but not-stimulated N11 cells, either in the form of direct protection or of significant additional protection over that afforded by lactose. Mannose receptor expression by N11 cells was negative before, and positive after, treatment with endotoxin, as assessed by the specific binding of 125I-mannose-bovine serum albumin. Moreover, a partial protection from ricin toxicity by mannan was induced in the N11 microglial line after stimulation, consistently with an inducible expression of the mannose receptor by activated cells switched towards a microglial phenotype. 相似文献
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