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Floriane Almire Selim Terhzaz Sandra Terry Melanie McFarlane Rommel J. Gestuveo Agnieszka M. Szemiel Margus Varjak Alma McDonald Alain Kohl Emilie Pondeville 《PLoS pathogens》2021,17(9)
As mosquito females require a blood meal to reproduce, they can act as vectors of numerous pathogens, such as arboviruses (e.g. Zika, dengue and chikungunya viruses), which constitute a substantial worldwide public health burden. In addition to blood meals, mosquito females can also take sugar meals to get carbohydrates for their energy reserves. It is now recognised that diet is a key regulator of health and disease outcome through interactions with the immune system. However, this has been mostly studied in humans and model organisms. So far, the impact of sugar feeding on mosquito immunity and in turn, how this could affect vector competence for arboviruses has not been explored. Here, we show that sugar feeding increases and maintains antiviral immunity in the digestive tract of the main arbovirus vector Aedes aegypti. Our data demonstrate that the gut microbiota does not mediate the sugar-induced immunity but partly inhibits it. Importantly, sugar intake prior to an arbovirus-infected blood meal further protects females against infection with arboviruses from different families. Sugar feeding blocks arbovirus initial infection and dissemination from the gut and lowers infection prevalence and intensity, thereby decreasing the transmission potential of female mosquitoes. Finally, we show that the antiviral role of sugar is mediated by sugar-induced immunity. Overall, our findings uncover a crucial role of sugar feeding in mosquito antiviral immunity which in turn decreases vector competence for arboviruses. Since Ae. aegypti almost exclusively feed on blood in some natural settings, our findings suggest that this lack of sugar intake could increase the spread of mosquito-borne arboviral diseases. 相似文献
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Jan-Philipp Bach Heike Borta Waltraud Ackermann Floriane Faust Oliver Borchers Michael Schrader 《The journal of histochemistry and cytochemistry》2006,54(8):877-888
The secretory granule protein syncollin was first identified in the exocrine pancreas where a population of the protein is associated with the luminal surface of the zymogen granule membrane. In this study we provide first morphological and biochemical evidence that, in addition to its pancreatic localization, syncollin is also present in neutrophilic granulocytes of rat and human origin. By immunohistological studies, syncollin was detected in neutrophilic granulocytes of the spleen. Furthermore, syncollin is expressed by the promyelocytic HL-60 cells, where it is stored in azurophilic granules and in a vesicular compartment. These findings were confirmed by fractionation experiments and immunoelectron microscopy. Treatment with a phorbol ester triggered the release of syncollin indicating that in HL-60 cells it is a secretory protein that can be mobilized upon stimulation. A putative role for syncollin in host defense is discussed. 相似文献
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Romain Bourcier Solena Le Scouarnec Stéphanie Bonnaud Matilde Karakachoff Emmanuelle Bourcereau Sandrine Heurtebise-Chrétien Céline Menguy Christian Dina Floriane Simonet Alexis Moles Cédric Lenoble Pierre Lindenbaum Stéphanie Chatel Bertrand Isidor Emmanuelle Génin Jean-François Deleuze Jean-Jacques Schott Hervé Le Marec 《American journal of human genetics》2018,102(1):133-141
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Anthony Drecourt Joël Babdor Michael Dussiot Floriane Petit Nicolas Goudin Meriem Garfa-Traoré Florence Habarou Christine Bole-Feysot Patrick Nitschké Chris Ottolenghi Metodi D. Metodiev Valérie Serre Isabelle Desguerre Nathalie Boddaert Olivier Hermine Arnold Munnich Agnès Rötig 《American journal of human genetics》2018,102(2):266-277
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Marta Érica Saidel Karen C. dos Santos Luís F.P. Nagano Carlos Alberto Montanari Andrei Leitão 《Bioorganic & medicinal chemistry letters》2017,27(17):4001-4006
Phenotypic assays were performed in prostate cancer cell lines to describe the biological activity of PI3K-AKT-mTOR pathway inhibitors retrieved from the virtual screening initiative. These novel chemicals share in common the aminopyridine scaffold, hitting PC-3 cells in macromolar range, with selectivity index over fibroblast cell lines. Moreover, a preliminary study of the mode of action by flow cytometry assay pointed out that these compounds had a rapamycin-like response for the PI3K-AKT-mTOR pathway modulation. 相似文献
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Francesca Patrignani Chiara Montanari Diana I. Serrazanetti Giacomo Braschi Pamela Vernocchi Giulia Tabanelli Giuseppina P. Parpinello Andrea Versari Fausto Gardini Rosalba Lanciotti 《Annals of microbiology》2017,67(1):99-109
Wine quality is closely linked to the fermentation step, which is driven by the microbial ecology of grape and the use of selected microbial strains as well. The microbial species developing during fermentation determines the type and concentration of many substances, which contribute to the sensory properties of wine and its safety. In this view, the present work aims to characterise the yeast microbiota, chemical and sensory properties of Sangiovese red wines obtained from both biodynamic and organic agriculture. The natural yeast populations of grape musts and their evolution during spontaneous were monitored and investigated. In addition, the volatile composition, physico-chemical and safety features (ethyl-carbamate) and sensory properties of wines were evaluated. The results showed that the yeast population was mostly related to the grape management, i.e. organic or biodynamic, while the wine composition was mainly affected by the winemaking process, and then by the grape management. 相似文献