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951.
Competition between microorganisms and arthropods has been shown to be an important ecological interaction determining animal
development and spatial distribution patterns in saprophagous communities. In fruit-inhabiting Drosophila, variation in insect developmental success is not only determined by species-specific effects of various noxious filamentous
fungi but, as suggested by an earlier study, also by additive genetic variation in the ability to successfully withstand the
negative impact of the fungi. If this variation represents a direct adaptive response to the degree to which insect breeding
substrates are infested with harmful fungi, genetic variation for successful development in the presence of fungi could be
maintained by variation in infestation of resource patches with fungi. We selected for the ability to resist the negative
influence of mould by maintaining replicated Drosophila melanogaster populations on substrates infested with Aspergillus nidulans. After five cycles of exposure to the fungus during the larval stage, the selected populations were compared with unselected
control populations regarding adult survival and reproduction to reveal an evolved resistance against the fungal competitor.
On fungus-infested larval feeding substrates, emerged adults from mould-selected populations had higher survival rates and
higher early fecundity than the control populations. In the unselected populations, females had higher mortality rates than
males, and a high proportion of both females and males appeared to be unable to lay eggs or fertilise eggs, respectively.
When larvae developed on non-infested food we found indications of a loss of resistance to abiotic and starvation stress in
the adult stage in flies from the selected populations. This suggests that there are costs associated with an increase in
resistance against the microbial competitor. We discuss the underlying mechanisms that might have selected for increased resistance
against harmful fungi. 相似文献
952.
Seven cyanopyridone derivatives and one corresponding seco compound have been isolated from a methanolic extract of the inflorescences and leaves of Acalypha indica L. (Euphorbiaceae). The absolute configuration of the main cyanogenic glucoside acalyphin, (−)-(5R,6S)-5-cyano-5-β-d-glucopyranosyloxy-6-hydroxy-4-methoxy-1-methyl-2(5,6-dihydro)-pyridone, was deduced from an X-ray crystallographic study. In addition, the 6R-epimer of acalyphin, epiacalyphin, and the corresponding pair of N-demethyl derivatives were isolated. The corresponding amide of acalyphin and a 1′,2′-glucosyl-fused epiacalyphin amide were isolated from air-dried material. Structural elucidation was performed by means of 1H and 13C NMR-spectra, chiroptical methods such as CD-spectroscopy and optical rotation. Two further corresponding derivatives, an aromatized compound and an open-chain structure, were isolated from the aqueous phase. 相似文献
953.
Agnieszka Bajda Dorota Konopka-Postupolska Magdalena Krzymowska Jacek Hennig Karolina Skorupinska-Tudek Liliana Surmacz Jacek Wójcik Zdzislaw Matysiak Tadeusz Chojnacki Ewa Skorzynska-Polit Maria Drazkiewicz Pawel Patrzylas Monika Tomaszewska Magdalena Kania Malgorzata Swist Witold Danikiewicz Wieslawa Piotrowska and Ewa Swiezewska 《Physiologia plantarum》2009,135(4):351-364
Infection with avirulent pathogens, tobacco mosaic virus (TMV) or Pseudomonas syringae pv. tabaci induced accumulation of polyisoprenoid alcohols, solanesol and a family of polyprenols [from polyprenol composed of 14 isoprene units (Pren-14) to -18, with Pren-16 dominating] in the leaves of resistant tobacco plants Nicotiana tabacum cv. Samsun NN. Upon TMV infection, solanesol content was increased seven- and eight-fold in the inoculated and upper leaves, respectively, while polyprenol content was increased 2.5- and 2-fold in the inoculated and upper leaves, respectively, on the seventh day post-infection. Accumulation of polyisoprenoid alcohols was also stimulated by exogenously applied hydrogen peroxide but not by exogenous salicylic acid (SA). On the contrary, neither inoculation of the leaves of susceptible tobacco plants nor wounding of tobacco leaves caused an increase in polyisoprenoid content. Taken together, these results indicate that polyisoprenoid alcohols might be involved in plant resistance against pathogens. A putative role of accumulated polyisoprenoids in plant response to pathogen attack is discussed. Similarly, the content of plastoquinone (PQ) was increased two-fold in TMV-inoculated and upper leaves of resistant plants. Accumulation of PQ was also stimulated by hydrogen peroxide, bacteria ( P. syringae ) and SA. The role of PQ in antioxidant defense in cellular membranous compartments is discussed in the context of the enzymatic antioxidant machinery activated in tobacco leaves subjected to viral infection. Elevated activity of several antioxidant enzymes (ascorbate peroxidase, guaiacol peroxidase, glutathione reductase and superoxide dismutase, especially the CuZn superoxide dismutase isoform) and high, but transient elevation of catalase was found in inoculated leaves of resistant tobacco plants but not in susceptible plants. 相似文献
954.
Possible participation of mitochondria in lipid yolk formation in oocytes of paddlefish and sturgeon 总被引:1,自引:0,他引:1
The ovary of paddlefish and sturgeons (Acipenseriformes) is composed of discrete units: the ovarian nests and ovarian follicles.
The ovarian nests comprise oogonia and numerous early dictyotene oocytes surrounded by somatic prefollicular cells. Each ovarian
follicle consists of a spherical oocyte and a layer of follicular cells situated on a thick basal lamina, encompassed by thecal
cells. The cytoplasm of previtellogenic oocytes is differentiated into two distinct zones: the homogeneous and granular zones.
The homogeneous cytoplasm is organelle-free, whereas the granular cytoplasm contains numerous organelles, including mitochondria
and lipid droplets. We have analyzed the cytoplasm of early dictyotene and previtellogenic oocytes ultrastructurally and histologically.
In the cytoplasm of early dictyotene oocytes, two morphologically different types of mitochondria can be distinguished: (1)
with well-developed cristae and (2) with distorted and fused cristae. In previtellogenic oocytes, the mitochondria of the
second type show various stages of cristae distortion; they contain and release material morphologically similar to that of
lipid droplets and eventually degenerate. This process of mitochondrial transformation is accompanied by an accumulation of
lipid droplets that form a single large accumulation (lipid body) located in the vicinity of the oocyte nucleus (germinal
vesicle). The lipid body eventually disperses in the oocyte center. The possible participation of these mitochondria in the
formation of oocyte lipid droplets is discussed.
This work was supported by funds from the research grant BW/IZ/2005 to M.Ż.
An erratum to this article can be found at http://dx.doi.org/.
An erratum to this article can be found at 相似文献
955.
Aneta Monika Białkowska Hubert Cieśliński Karolina Maria Nowakowska Józef Kur Marianna Turkiewicz 《Archives of microbiology》2009,191(11):825-835
A psychrotrophic bacterium producing a cold-adapted β-galactosidase upon growth at low temperatures was classified as Arthrobacter sp. 20B. A genomic DNA library of strain 20B introduced into Escherichia coli TOP10F′ and screening on X-Gal (5-bromo-4-chloro-3-indolyl-β-d-galactopyranoside)-containing agar plates led to the isolation of β-galactosidase gene. The β-galactosidase gene (bgaS) encoding a protein of 1,053 amino acids, with a calculated molecular mass of 113,695 kDa. Analysis of the amino acid sequence
of BgaS protein, deduced from the bgaS ORF, suggested that it is a member of the glycosyl hydrolase family 2. A native cold-adapted β-galactosidase was purified
to homogeneity and characterized. It is a homotetrameric enzyme, each subunit being approximately 116 kDa polypeptide as deduced
from native and SDS–PAGE, respectively. The β-galactosidase was optimally active at pH 6.0–8.0 and 25°C. P-nitrophenyl-β-d-galactopyranoside (PNPG) is its preferred substrate (three times higher activity than for ONPG—o-nitrophenyl-β-d-galactopyranoside). The Arthrobacter sp. 20B β-galactosidase is activated by thiol compounds (53% rise in activity in the presence of 10 mM 2-mercaptoethanol),
some metal ions (activity increased by 50% for Na+, K+ and by 11% for Mn2+) and inactivated by pCMB (4-chloro-mercuribenzoic acid) and heavy metal ions (Pb2+, Zn2+, Cu2+). 相似文献
956.
Ulrike Kloiber Bettina Pflugfelder Christian Rinke Monika Bright 《Symbiosis (Philadelphia, Pa.)》2009,47(1):43-50
Chemolithoautotrophic, sulphide-oxidizing (thiotrophic) symbioses represent spectacular adaptations to fluctuating environmental
gradients and survival is often accomplished when growth is fuelled by sufficient nourishment through the symbionts leading
to fast cell proliferation. Here we show 5′-bromo-2′ deoxyuridine (BrdU) pulse labelling of vegetative growingZoothamnium niveum, a colonial ciliate obligately associated with thiotrophic ectosymbionts, and demonstrate age related growth profiles in
three heteromorphic host cell types. At the colony’s apex, a large top terminal zooid performed high proliferation activity,
which decreased significantly with increasing colony age but was still present in old colonies indicating that this cell possesses
lifelong cell division potential. In contrast, terminal branch zooids proliferated independent of colony age but appeared
to be limited by their cell division capacity predetermined by branch size, thus leading to the strict, feather-shaped colony
form. Appearance of labelled terminal branch zooids allowed us to distinguish a highly proliferating apical colony region
from an almost inactive, senescent basal region. In macrozooids attached to the colony, extensive BrdU labelling suggests
that DNA synthesis occurs in preparation for a new generation. As motile swarmers, the macrozooids seem to be arrested in
the cell cycle and mitosis and cell division occur when the swarmer settles and transforms into a top terminal zooid buildingup
a new colony. 相似文献
957.
Christian Rinke Stephan Schmitz-Esser Alexander Loy Matthias Horn Michael Wagner & Monika Bright 《FEMS microbiology ecology》2009,67(2):229-241
The giant marine ciliate Zoothamnium niveum ( Ciliophora, Oligohymenophora ) is obligatorily covered by a monolayer of putative chemoautotrophic sulfur-oxidizing (thiotrophic) bacteria. For Z. niveum specimens from the Caribbean Sea it has been demonstrated that this ectosymbiotic population consists of only a single pleomorphic phylotype described as Candidatus Thiobios zoothamnicoli. The goal of our study was to identify and phylogenetically analyse the ectosymbiont(s) of a recently discovered Z. niveum population from the Mediterranean Sea, and to compare marker genes encoding key enzymes of the carbon and sulfur metabolism between the two symbiont populations. We identified a single bacterial phylotype representing the ectosymbiont of Z. niveum from the Mediterranean population showing 99.7% 16S rRNA gene (99.2% intergenic spacer region) similarity to the Caribbean Z. niveum ectosymbiont. Genes encoding enzymes typical for an inorganic carbon metabolism [ribulose 1,5-bisphosphate carboxylase/oxygenase (RuBisCO)] and for sulfur metabolism (5'-adenylylsulfate reductase, dissimilatory sulfite reductase) were detected in both symbiotic populations. The very high amino acid sequence identity (97–100%) and the high nucleic acid sequence identity (90–98%) of these marker enzymes in two geographically distant symbiont populations suggests that the association of Z. niveum with Cand . Thiobios zoothamnicoli is very specific as well as temporally and spatially stable. 相似文献
958.
959.
Yasuhiro Yamauchi Monika A. Ward W. Steven Ward 《Journal of cellular biochemistry》2009,107(2):214-223
To prevent duplicate DNA synthesis, metazoan replication origins are licensed during G1. Only licensed origins can initiate replication, and the cytoplasm interacts with the nucleus to inhibit new licensing during S phase. DNA replication in the mammalian one‐cell embryo is unique because it occurs in two separate pronuclei within the same cytoplasm. Here, we first tested how long after activation the oocyte can continue to support licensing. Because sperm chromatin is licensed de novo after fertilization, the timing of sperm injection can be used to assay licensing initiation. To experimentally skip some of the steps of sperm decondensation, we injected mouse sperm halos into parthenogenetically activated oocytes. We found that de novo licensing was possible for up to 3 h after oocyte activation, and as early as 4 h before DNA replication began. We also found that the oocyte cytoplasm could support asynchronous initiation of DNA synthesis in the two pronuclei with a difference of at least 2 h. We next tested how tightly the oocyte cytoplasm regulates DNA synthesis by transferring paternal pronuclei from zygotes generated by intracytoplasmic sperm injection (ICSI) into parthenogenetically activated oocytes. The pronuclei from G1 phase zygotes transferred into S phase ooplasm were not induced to prematurely replicate and paternal pronuclei from S phase zygotes transferred into G phase ooplasm continued replication. These data suggest that the one‐cell embryo can be an important model for understanding the regulation of DNA synthesis. J. Cell. Biochem. 107: 214–223, 2009. © 2009 Wiley‐Liss, Inc. 相似文献
960.
Nicole Rebscher Christina Deichmann Stefanie Sudhop Jens Holger Fritzenwanker Stephen Green Monika Hassel 《Development genes and evolution》2009,219(9-10):455-468
We have analyzed the evolution of fibroblast growth factor receptor (FGFR) tyrosine kinase genes throughout a wide range of animal phyla. No evidence for an FGFR gene was found in Porifera, but we tentatively identified an FGFR gene in the placozoan Trichoplax adhaerens. The gene encodes a protein with three immunoglobulin-like domains, a single-pass transmembrane, and a split tyrosine kinase domain. By superimposing intron positions of 20 FGFR genes from Placozoa, Cnidaria, Protostomia, and Deuterostomia over the respective protein domain structure, we identified ten ancestral introns and three conserved intron groups. Our analysis shows (1) that the position of ancestral introns correlates to the modular structure of FGFRs, (2) that the acidic domain very likely evolved in the last common ancestor of triploblasts, (3) that splicing of IgIII was enabled by a triploblast-specific insertion, and (4) that IgI is subject to substantial loss or duplication particularly in quickly evolving genomes. Moreover, intron positions in the catalytic domain of FGFRs map to the borders of protein subdomains highly conserved in other serine/threonine kinases. Nevertheless, these introns were introduced in metazoan receptor tyrosine kinases exclusively. Our data support the view that protein evolution dating back to the Cambrian explosion took place in such a short time window that only subtle changes in the domain structure are detectable in extant representatives of animal phyla. We propose that the first multidomain FGFR originated in the last common ancestor of Placozoa, Cnidaria, and Bilateria. Additional domains were introduced mainly in the ancestor of triploblasts and in the Ecdysozoa. 相似文献