全文获取类型
收费全文 | 5234篇 |
免费 | 418篇 |
国内免费 | 1篇 |
出版年
2023年 | 24篇 |
2022年 | 51篇 |
2021年 | 124篇 |
2020年 | 56篇 |
2019年 | 87篇 |
2018年 | 104篇 |
2017年 | 94篇 |
2016年 | 160篇 |
2015年 | 292篇 |
2014年 | 281篇 |
2013年 | 374篇 |
2012年 | 453篇 |
2011年 | 433篇 |
2010年 | 268篇 |
2009年 | 205篇 |
2008年 | 340篇 |
2007年 | 321篇 |
2006年 | 285篇 |
2005年 | 281篇 |
2004年 | 270篇 |
2003年 | 232篇 |
2002年 | 247篇 |
2001年 | 53篇 |
2000年 | 43篇 |
1999年 | 48篇 |
1998年 | 56篇 |
1997年 | 55篇 |
1996年 | 21篇 |
1995年 | 39篇 |
1994年 | 36篇 |
1993年 | 41篇 |
1992年 | 29篇 |
1991年 | 18篇 |
1990年 | 17篇 |
1989年 | 22篇 |
1988年 | 17篇 |
1987年 | 10篇 |
1986年 | 13篇 |
1985年 | 16篇 |
1984年 | 11篇 |
1983年 | 8篇 |
1982年 | 19篇 |
1981年 | 12篇 |
1980年 | 7篇 |
1979年 | 7篇 |
1978年 | 6篇 |
1976年 | 10篇 |
1975年 | 7篇 |
1973年 | 7篇 |
1969年 | 11篇 |
排序方式: 共有5653条查询结果,搜索用时 15 毫秒
921.
922.
Ormeño-Orrillo E Rosenblueth M Luyten E Vanderleyden J Martínez-Romero E 《Environmental microbiology》2008,10(5):1271-1284
Three transposon mutants of Rhizobium tropici CIAT899 affected in lipopolysaccharide (LPS) biosynthesis were characterized and their maize rhizosphere and endophytic root colonization abilities were evaluated. The disrupted genes coded for the following putative products: the ATPase component of an O antigen ABC-2 type transporter ( wzt ), a nucleotide-sugar dehydratase ( lpsβ2 ) and a bifunctional enzyme producing GDP-mannose ( noeJ ). Electrophoretic analysis of affinity purified LPS showed that all mutants lacked the smooth LPS bands indicating an O antigen minus phenotype. In the noeJ mutant, the rough LPS band migrated faster than the parental band, suggesting a truncated LPS core. When inoculated individually, the wzt and noeJ mutants colonize the rhizosphere and root to a lower extent than the parental strain while no differences were observed between the lpsβ2 mutant and the parental strain. All mutants were impaired in competitive rhizosphere and root colonization. Pleiotropic effects of the mutations on known colonization traits such as motility and growth rate were observed, but they were not sufficient to explain the colonization behaviours. It was found that the LPS mutants were sensitive to the maize antimicrobial 6-methoxy-2-benzoxazolinone (MBOA). Only the combined effects of altered growth rate and susceptibility to maize antimicrobials could account for all the observed colonization phenotypes. The results suggest an involvement of the LPS in protecting R. tropici against maize defence response during rhizosphere and root colonization. 相似文献
923.
In addition to the important role of abscisic acid (ABA) in abiotic stress signalling, basal and high ABA levels appear to have a negative effect on disease resistance. Using the ABA-deficient sitiens tomato ( Solanum lycopersicum ) mutant and different application methods of exogenous ABA, we demonstrated the influence of this plant hormone on disease progression of Erwinia chrysanthemi . This necrotrophic plant pathogenic bacterium is responsible for soft rot disease on many plant species, causing maceration symptoms mainly due to the production and secretion of pectinolytic enzymes. On wild-type (WT) tomato cv. Moneymaker E. chrysanthemi leaf inoculation resulted in maceration both within and beyond the infiltrated zone of the leaf, but sitiens showed a very low occurrence of tissue maceration, which never extended the infiltrated zone. A single ABA treatment prior to infection eliminated the effect of pathogen restriction in sitiens , while repeated ABA spraying during plant development rendered both WT and sitiens very susceptible. Quantification of E. chrysanthemi populations inside the leaf did not reveal differences in bacterial growth between sitiens and WT. Sitiens was not more resistant to pectinolytic cell-wall degradation, but upon infection it showed a faster and stronger activation of defence responses than WT, such as hydrogen peroxide accumulation, peroxidase activation and cell-wall fortifications. Moreover, the rapid activation of sitiens peroxidases was also observed after application of bacteria-free culture filtrate containing E. chrysanthemi cell-wall-degrading enzymes and was absent during infection with an out E. chrysanthemi mutant impaired in secretion of these extracellular enzymes. 相似文献
924.
Harriet Watkin Monica M Richert Andrew Lewis Kristina Terrell James P McManaman Steven M Anderson 《BMC developmental biology》2008,8(1):6
Background
Mammary gland development culminates in lactation and is orchestrated by numerous stimuli and signaling pathways. The Src family of nonreceptor tyrosine kinases plays a pivotal role in cell signaling. In order to determine if Src plays a role in mammary gland development we have examined mammary gland development and function during pregnancy and lactation in mice in which expression of Src has been eliminated. 相似文献925.
Xia M Hou C DeMong D Pollack S Pan M Singer M Matheis M Murray W Cavender D Wachter M 《Bioorganic & medicinal chemistry letters》2008,18(24):6468-6470
The synthesis and structure-activity relationship of a series of 7-azaindole piperidine derivatives are described. SAR studies led to the discovery of the potent CCR2 antagonists displaying IC(50) values in the nanomolar range. The representative compound 15 showed reasonable P450 and pharmacokinetics profile. 相似文献
926.
Transplastomic tobacco plants expressing a fatty acid desaturase gene exhibit altered fatty acid profiles and improved cold tolerance 总被引:4,自引:0,他引:4
927.
Martini E Norcini M Ghelardini C Manetti D Dei S Guandalini L Melchiorre M Pagella S Scapecchi S Teodori E Romanelli MN 《Bioorganic & medicinal chemistry》2008,16(23):10034-10042
A series of amides, structurally related to DM232 (unifiram) and DM235 (sunifiram), characterized by a 1,2,3,4-tetrahydropyrazino[2,1-a]isoindol-6(2H)-one, 1,4-diamino-cyclohexane or 1,4-diaminobenzene ring, have been synthesized and tested for cognition-enhancing activity in the mouse passive-avoidance test. Some of the compounds display good antiamnesic and procognitive activity, with higher potency than piracetam, while some cyclohexane derivatives are endowed with amnesia inducing properties. 相似文献
928.
Biava M Porretta GC Poce G Supino S Manetti F Forli S Botta M Sautebin L Rossi A Pergola C Ghelardini C Norcini M Makovec F Giordani A Anzellotti P Cirilli R Ferretti R Gallinella B La Torre F Anzini M Patrignani P 《Bioorganic & medicinal chemistry》2008,16(17):8072-8081
Following our previous research on anti-inflammatory drugs (NSAIDs), we report here the synthesis of chiral 1,5-diarylpyrroles derivatives that were characterized for their in vitro inhibitory effects toward cyclooxygenase (COX) isozymes. Analysis of enzymatic affinity and COX-2 selectivity led us to the selection of one compound (+/-)-10b that was further tested in vitro in the human whole blood (HWB) and in vivo for its anti-inflammatory activity in mice. The affinity data have been rationalized through docking simulations. 相似文献
929.
Guerrini G Ciciani G Cambi G Bruni F Selleri S Melani F Montali M Martini C Ghelardini C Norcini M Costanzo A 《Bioorganic & medicinal chemistry》2008,16(8):4471-4489
The synthesis and binding studies of a series of 3-acylpyrazolo[5,1-c][1,2,4]benzotriazine 5-oxides 8-substituted are reported. High-affinity ligands at benzodiazepine site on GABA(A) receptor complex (GABA(A)/BzR complex) were obtained when the 3-aroyl substituent is represented by a five-member heteroaroyl ring (furoyl-, thenoyl-, and pyrroyl-). Moreover the type of heteroaroyl ring at position 3 influences the feature of the substituent at position 8 to obtain high-affinity ligands: a 'hydrogen-bond acceptor ring' at position 3 is synergic with an electron donor substituent at position 8, while a 'hydrogen-bond donor ring' is synergic with a withdrawing substituent. Compounds 8a, 9b, and 11 were deeply studied in vivo for their pharmacological effects considering six potential benzodiazepine actions: motor coordination, anticonvulsant action, spontaneous motor activity and explorative activity, anxiolytic-like effects, mouse learning and memory modulation, and ethanol-potentiating action. To rationalize and qualitatively interpret the GABA(A)/Bz binding affinities of compounds 8a and 11, a dynamic molecular modeling study has been performed, with the aim of assessing the preferred geometry of protein-ligand complex. 相似文献
930.
Banfi L Basso A Bevilacqua E Gandolfo V Giannini G Guanti G Musso L Paravidino M Riva R 《Bioorganic & medicinal chemistry》2008,16(7):3501-3518
Lactenediynes are compounds characterized by the fusion of a beta-lactam with a cyclodeca-3-ene-1,5-diyne. In this work the most promising members of this family have been activated by attaching a carbalkoxy or a carbamoyl group to the azetidinone nitrogen, and conjugated to various DNA-complexing moieties, either acting by intercalation or through groove binding. These conjugated artificial enediynes have been demonstrated to possess in vitro ability to produce single and double strand cleavage of plasmid DNA. As potency and capacity to induce double cut, they rank among the best simple enediyne analogues ever prepared. A thorough investigation was carried out in order to develop the best suited linkers for assembling these conjugates. 相似文献