全文获取类型
收费全文 | 4758篇 |
免费 | 380篇 |
国内免费 | 1篇 |
专业分类
5139篇 |
出版年
2023年 | 25篇 |
2022年 | 64篇 |
2021年 | 119篇 |
2020年 | 51篇 |
2019年 | 73篇 |
2018年 | 98篇 |
2017年 | 85篇 |
2016年 | 147篇 |
2015年 | 261篇 |
2014年 | 243篇 |
2013年 | 329篇 |
2012年 | 417篇 |
2011年 | 395篇 |
2010年 | 247篇 |
2009年 | 194篇 |
2008年 | 306篇 |
2007年 | 298篇 |
2006年 | 263篇 |
2005年 | 257篇 |
2004年 | 242篇 |
2003年 | 218篇 |
2002年 | 237篇 |
2001年 | 42篇 |
2000年 | 34篇 |
1999年 | 47篇 |
1998年 | 53篇 |
1997年 | 46篇 |
1996年 | 20篇 |
1995年 | 36篇 |
1994年 | 32篇 |
1993年 | 35篇 |
1992年 | 25篇 |
1991年 | 14篇 |
1990年 | 16篇 |
1989年 | 18篇 |
1988年 | 10篇 |
1987年 | 7篇 |
1986年 | 8篇 |
1985年 | 15篇 |
1984年 | 10篇 |
1983年 | 7篇 |
1982年 | 17篇 |
1981年 | 10篇 |
1980年 | 7篇 |
1979年 | 6篇 |
1978年 | 5篇 |
1976年 | 10篇 |
1973年 | 4篇 |
1972年 | 4篇 |
1969年 | 5篇 |
排序方式: 共有5139条查询结果,搜索用时 15 毫秒
81.
Nuria Mencia-Trinchant Matthew J. MacKay Christopher Chin Ebrahim Afshinnekoo Jonathan Foox Cem Meydan Daniel Butler Christopher Mozsary Nicholas A. Vernice Charlotte Darby Michael C. Schatz Susan M. Bailey Ari M. Melnick Monica L. Guzman Kelly Bolton Lior Z. Braunstein Francine Garrett-Bakelman Ross L. Levine Christopher E. Mason 《Cell reports》2021,34(6):108740
82.
Roberta Bonfiglioli Patrizia Mussoni Francesca Graziosi Monica Calabrese Andrea Farioli Francesco Marinelli Francesco S. Violante 《Journal of electromyography and kinesiology》2013,23(1):252-259
To test whether the influence of manual activity should be considered when interpreting the results of nerve conduction study (NCS) of the upper limbs performed during work shifts, we evaluated the short-term effect of 90-min repetitive manual work on NCS parameters. Twenty-eight healthy volunteers underwent NCS of the dominant limb at the end of an interview (T0), after a 30-min rest in sitting position (T1) and after performing a standardized 90-min manual task (T2). The task was designed to simulate typical assembly and packing activities. No significant differences were observed for skin temperature (Ts) and NCS parameters between T0 and T1. Significantly (p < 0.001) higher Ts mean values were found at T2 as compared to the previous tests for both females and males. The regression analysis showed an association between temperature variation and nerve conduction velocity values for the median and ulnar nerve at T2 as compared to T1. In females, a reduction of the mean sensory nerve action potential (SNAP) amplitude at T2 was recorded, whereas an opposite trend was observed among males. Manual work is able to influence hand Ts and to modify NCS parameters. SNAP amplitudes changes suggest gender differences in peripheral nerve characteristics that deserve further investigation. 相似文献
83.
N-acetylcysteine Protects Mice from Lethal Endotoxemia by Regulating the Redox State of Immune Cells
The excessive production of reactive oxygen species (ROS) associated with inflammation leads to oxidative stress, which is involved with the high mortality from several diseases such as endotoxic shock and can be controlled to a certain degree by antioxidants. The immune cells use ROS in order to support their functions and, therefore, need adequate levels of antioxidant defenses in order to avoid the harmful effect of an excessive ROS production. In the present work, the effect of the administration of the antioxidant N-acetylcysteine (NAC) on the redox state of peritoneal macrophages and lymphocytes from mice with lethal endotoxic shock (100 mg/kg i.p. of lipopolysaccharide, LPS), was studied. In both types of immune cells at 0, 2, 4, 12 and 24 h after LPS injection, an increase of ROS, of the proinflammatory cytokine tumor necrosis factor alpha (TNFα), the lipid peroxidation (malonaldehyde levels, MDA), inducible nitric oxide synthase (iNOS) expression and the oxidized/reduced glutathione (GSSG/GSH) ratio, as well as a decrease of enzymatic antioxidant defenses, such as superoxide dismutase (SOD) and catalase (CAT) activity, was observed. The injection of NAC (150 mg/kg i.p. at 30 min after LPS injection) decreased the ROS, the TNFα the MDA levels, iNOS expression and the GSSG/GSH ratio, and increased the antioxidant defenses in both macrophages and lymphocytes. Moreover, the NAC treatment prevented the activation of nuclear translocation of the nuclear factor κB (NF-κB), which regulates ROS, inflammatory cytokines and antioxidant levels. Our present results provide evidence that both cell types have a relevant role in the pathogenesis of endotoxic shock, and that NAC, by improving the redox state of these immune cells, could increase mouse survival. Thus, antioxidants could offer an alternative treatment of human endotoxic shock. 相似文献
84.
85.
Rosa Tundis Federica Menichini Filomena Conforti Monica R. Loizzo Marco Bonesi Giancarlo Statti 《Journal of enzyme inhibition and medicinal chemistry》2013,28(3):818-824
From the aerial parts of Salsola oppositofolia, S. soda and S. tragus an alkaloid extract was obtained and tested to evaluate antioxidant and anti-cholinesterase activities. The in vitro study of the antioxidant activity by the DPPH method revealed a significant activity of Salsola alkaloid extracts with IC50 values ranging from 16.30 μg/mL for S. oppositifolia to 26.17 μg/mL for S. tragus. Acetylcholinesterase (AChE) and butyrylcholinesterase (BChE) inhibitory activities were evaluated. S. tragus alkaloid extract exerted the highest inhibitory activity against AChE (IC50 of 30.2 μg/mL) and BChE (IC50 of 26.5 μg/mL). Interestingly, S. soda and S. oppositifolia exhibited a selective inhibitory activity against BChE with IC50 values of 34.3 μg/mL and 32.7 μg/mL, respectively. Tetrahydroisoquinoline alkaloids were identified and quantified by GC/MS analysis. 相似文献
86.
Sylvie Pochet Laurence Dugué Monica Sala Valérie Pézo Simon Wain-Hobson 《Nucleosides, nucleotides & nucleic acids》2013,32(7-9):1749-1752
Abstract The use of 5′-triphosphate of 1-(2-deoxy-β-D-ribofuranosyl)imidazole-4-carboxamide (dYTP) in DNA amplification reaction in place of dATP or dGTP yielded mutations frequencies of 3–4×10?2 per base per amplification. A reasonable proportion of transversions (11–15%) was observed in the absence of deletions and insertions. 相似文献
87.
P. L. C. Walker Adele Corrigan Monica Arenas Emilia Escuredo Lynette Fairbanks Anthony Marinaki 《Nucleosides, nucleotides & nucleic acids》2013,32(12):1243-1247
Purine nucleoside phosphorylase (PNPase) deficiency is an autosomal recessive disorder affecting purine degradation and salvage pathways. Clinically, patients typically present with severe immunodeficiency, neurological dysfunction, and autoimmunity. Biochemically, PNPase deficiency may be suspected in the presence of hypouricemia. We report biochemical and genetic data on a cohort of seven patients from six families identified as PNPase deficient. In all patients, inosine, deoxyinosine, guanosine, and deoxyguanosine were elevated in urine, and mutation analysis revealed seven different mutations of which three were novel. The mutation c.770A>G resulted in the substitution p.His257Arg. A second novel mutation c.257A>G (p.His86Arg) was identified in two siblings and a third novel mutation, c.199C>T (p.Arg67X), was found in a 2-year-old female with delayed motor milestones and recurrent respiratory infections. A review of the literature identified 67 cases of PNPase deficiency from 49 families, including the cases from our own laboratory. PNPase deficiency was confirmed in 30 patients by genotyping and 24 disease causing mutations, including the three novel mutations described in this paper, have been reported to date. In five of the seven patients, plasma uric acid was found to be within the pediatric normal range, suggesting that PNPase deficiency should not be ruled out in the absence of hypouricemia. 相似文献
88.
89.
Adelaide Greco Monica Ragucci Anna Rita Daniela Coda Alessandro Rosa Sara Gargiulo Raffaele Liuzzi Matteo Gramanzini Sandra Albanese Sabina Pappatà Marcello Mancini Arturo Brunetti Marco Salvatore 《PloS one》2013,8(10)