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排序方式: 共有103条查询结果,搜索用时 15 毫秒
91.
92.
Florent Lefebvre-Pautigny Feinan Wu Murielle Philippot Michel Rigoreau Priyono Mohamed Zouine Pierre Frasse Mondher Bouzayen Pierre Broun Vincent Pétiard Steven D. Tanksley Dominique Crouzillat 《Tree Genetics & Genomes》2010,6(4):565-577
Tomato (Solanum lycopersicum) and coffee (Coffea canephora) belong to the sister families Solanaceae and Rubiaceae, respectively. We report herein the mapping of a common set of 257 Conserved Ortholog Set II genes in the genomes of both species. The mapped markers are well distributed across both genomes allowing the first syntenic comparison between species from these two families. The majority (75%) of the synteny blocks are short (<4 cM); however, some extend up to 50 cM. In an effort to further characterize the synteny between these two genomes, we took advantage of the available sequence for the tomato genome to show that tomato chromosome 7 is syntenic to half of the two coffee linkage groups E and F with the putative break point in tomato localized to the boundary of the heterochromatin and euchromatin on the long arm. In addition to the new insight on genome conservation and evolution between the plant families Solanaceae and Rubiaceae, the comparative maps presented herein provide a translational tool by which coffee researchers may take benefit of DNA sequence and genetic information from tomato and vice versa. It is thus expected that these comparative genome information will help to facilitate and expedite genetic and genomic research in coffee. 相似文献
93.
El-Sharkawy I Manríquez D Flores FB Regad F Bouzayen M Latché A Pech JC 《Plant molecular biology》2005,59(2):345-362
Volatile esters, a major class of compounds contributing to the aroma of many fruit, are synthesized by alcohol acyl-transferases
(AAT). We demonstrate here that, in Charentais melon (Cucumis melo var. cantalupensis), AAT are encoded by a gene family of at least four members with amino acid identity ranging from 84% (Cm-AAT1/Cm-AAT2) and
58% (Cm-AAT1/Cm-AAT3) to only 22% (Cm-AAT1/Cm-AAT4). All encoded proteins, except Cm-AAT2, were enzymatically active upon
expression in yeast and show differential substrate preferences. Cm-AAT1 protein produces a wide range of short and long-chain
acyl esters but has strong preference for the formation of E-2-hexenyl acetate and hexyl hexanoate. Cm-AAT3 also accepts a wide range of substrates but with very strong preference for
producing benzyl acetate. Cm-AAT4 is almost exclusively devoted to the formation of acetates, with strong preference for cinnamoyl
acetate. Site directed mutagenesis demonstrated that the failure of Cm-AAT2 to produce volatile esters is related to the presence
of a 268-alanine residue instead of threonine as in all active AAT proteins. Mutating 268-A into 268-T of Cm-AAT2 restored
enzyme activity, while mutating 268-T into 268-A abolished activity of Cm-AAT1. Activities of all three proteins measured
with the prefered substrates sharply increase during fruit ripening. The expression of all Cm-AAT genes is up-regulated during
ripening and inhibited in antisense ACC oxidase melons and in fruit treated with the ethylene antagonist 1-methylcyclopropene
(1-MCP), indicating a positive regulation by ethylene. The data presented in this work suggest that the multiplicity of AAT
genes accounts for the great diversity of esters formed in melon.
*Accession numbers Cm-AAT1 (CAA94432), Cm-AAT2 (AAL77060), Cm-AAT3 (AAW51125), and Cm-AAT4 (AAW51126).
Islam El-Sharkawy, Daniel Manriquez, Francisco B. Flores: These authors contributed equally to the work 相似文献
94.
SlARF4, an Auxin Response Factor Involved in the Control of Sugar Metabolism during Tomato Fruit Development 总被引:1,自引:0,他引:1
95.
Vandeputte O Oden S Mol A Vereecke D Goethals K El Jaziri M Prinsen E 《Applied and environmental microbiology》2005,71(3):1169-1177
The role and metabolism of indole-3-acetic acid in gram-negative bacteria is well documented, but little is known about indole-3-acetic acid biosynthesis and regulation in gram-positive bacteria. The phytopathogen Rhodococcus fascians, a gram-positive organism, incites diverse developmental alterations, such as leafy galls, on a wide range of plants. Phenotypic analysis of a leafy gall suggests that auxin may play an important role in the development of the symptoms. We show here for the first time that R. fascians produces and secretes the auxin indole-3-acetic acid. Interestingly, whereas noninfected-tobacco extracts have no effect, indole-3-acetic acid synthesis is highly induced in the presence of infected-tobacco extracts when tryptophan is not limiting. Indole-3-acetic acid production by a plasmid-free strain shows that the biosynthetic genes are located on the bacterial chromosome, although plasmid-encoded genes contribute to the kinetics and regulation of indole-3-acetic acid biosynthesis. The indole-3-acetic acid intermediates present in bacterial cells and secreted into the growth media show that the main biosynthetic route used by R. fascians is the indole-3-pyruvic acid pathway with a possible rate-limiting role for indole-3-ethanol. The relationship between indole-3-acetic acid production and the symptoms induced by R. fascians is discussed. 相似文献
96.
PtaRHE1, a Populus tremula × Populus alba RING‐H2 protein of the ATL family,has a regulatory role in secondary phloem fibre development 下载免费PDF全文
Fabien Baldacci‐Cresp Jihad Moussawi Jean‐Charles Leplé Rebecca Van Acker Annegret Kohler Julie Candiracci Laure Twyffels Antanas V. Spokevicius Gerd Bossinger Françoise Laurans Nicole Brunel Marjorie Vermeersch Wout Boerjan Mondher El Jaziri Marie Baucher 《The Plant journal : for cell and molecular biology》2015,82(6):978-990
REALLY INTERESTING NEW GENE (RING) proteins play important roles in the regulation of many processes by recognizing target proteins for ubiquitination. Previously, we have shown that the expression of PtaRHE1, encoding a Populus tremula × Populus alba RING‐H2 protein with E3 ubiquitin ligase activity, is associated with tissues undergoing secondary growth. To further elucidate the role of PtaRHE1 in vascular tissues, we have undertaken a reverse genetic analysis in poplar. Within stem secondary vascular tissues, PtaRHE1 and its corresponding protein are expressed predominantly in the phloem. The downregulation of PtaRHE1 in poplar by artificial miRNA triggers alterations in phloem fibre patterning, characterized by an increased portion of secondary phloem fibres that have a reduced cell wall thickness and a change in lignin composition, with lower levels of syringyl units as compared with wild‐type plants. Following an RNA‐seq analysis, a biological network involving hormone stress signalling, as well as developmental processes, could be delineated. Several candidate genes possibly associated with the altered phloem fibre phenotype observed in amiRPtaRHE1 poplar were identified. Altogether, our data suggest a regulatory role for PtaRHE1 in secondary phloem fibre development. 相似文献
97.
Biosynthesis of Auxin by the Gram-Positive Phytopathogen Rhodococcus fascians Is Controlled by Compounds Specific to Infected Plant Tissues 总被引:1,自引:0,他引:1 下载免费PDF全文
Olivier Vandeputte Sevgi ?den Adeline Mol Danny Vereecke Koen Goethals Mondher El Jaziri Els Prinsen 《Applied microbiology》2005,71(3):1169-1177
The role and metabolism of indole-3-acetic acid in gram-negative bacteria is well documented, but little is known about indole-3-acetic acid biosynthesis and regulation in gram-positive bacteria. The phytopathogen Rhodococcus fascians, a gram-positive organism, incites diverse developmental alterations, such as leafy galls, on a wide range of plants. Phenotypic analysis of a leafy gall suggests that auxin may play an important role in the development of the symptoms. We show here for the first time that R. fascians produces and secretes the auxin indole-3-acetic acid. Interestingly, whereas noninfected-tobacco extracts have no effect, indole-3-acetic acid synthesis is highly induced in the presence of infected-tobacco extracts when tryptophan is not limiting. Indole-3-acetic acid production by a plasmid-free strain shows that the biosynthetic genes are located on the bacterial chromosome, although plasmid-encoded genes contribute to the kinetics and regulation of indole-3-acetic acid biosynthesis. The indole-3-acetic acid intermediates present in bacterial cells and secreted into the growth media show that the main biosynthetic route used by R. fascians is the indole-3-pyruvic acid pathway with a possible rate-limiting role for indole-3-ethanol. The relationship between indole-3-acetic acid production and the symptoms induced by R. fascians is discussed. 相似文献
98.
99.
Maha Chamtouri Anuj Dhawan Mondher Besbes Julien Moreau Hassen Ghalila Tuan Vo-Dinh Michael Canva 《Plasmonics (Norwell, Mass.)》2014,9(1):79-92
In this study, we theoretically investigate the sensing potential of 2D nano- and micro-ribbon grating structuration on the surface of Kretschmann-based surface plasmon resonance (SPR) biosensors when they are employed for detection of biomolecular binding events. Numerical simulations were carried out by employing a model based on the hybridization of two classical methods, the Fourier modal method and the finite element method. Our calculations confirm the importance of light manipulation by means of structuration of the plasmonic thin film surfaces on the nano- and micro-scales. Not only does it highlight the geometric parameters that allow the sensitivity enhancement compared with the response of the conventional SPR biosensor based on a flat surface but also describes the transition from the regime where the propagating surface plasmon mode dominates to the regime where the localized surface plasmon mode dominates. An exhaustive mapping of the biosensing potential of the 2D nano- and micro-structured biosensors surface is presented, varying the structural parameters related to the ribbon grating dimensions, i.e., the widths and thicknesses. The nano- and micro-structuration also leads to the creation of regions on biosensor chips that are characterized by strongly enhanced electromagnetic (EM) fields. New opportunities for further improving the sensitivity are offered if localization of biomolecules can be carried out in these regions of high EM fields. The continuum of nano- and micro-ribbon structured biosensors described in this study should prove a valuable tool for developing sensitive and reliable 2D-structured plasmonic biosensors. 相似文献
100.
Romain Bazabakana Ruddy Wattiez Marie Baucher Billo Diallo Mondher Jaziri 《Plant Growth Regulation》2003,40(3):229-237
A developmental morphology study was performed during different stages of in vitro yam microtuber formation on both hormone-free medium (HF) and medium supplemented with 10 µM of jasmonic acid (JA). The axillary protuberance, considered as the first morphological evidence for microtuber formation, became visible after one-week of culture in JA-medium and after three weeks of culture in HF-medium. In addition, the formation of the axillary protuberance in JA-cultures preceded the stem elongation, whereas in HF-medium stem elongation was visible before the formation of the axillary protuberance. Tuberization improvement in medium supplemented with JA is likely to be the result of morphogenetic modifications occurring during the early stages of microtuber formation. At the molecular level, 2D-PAGE analysis of HF- and JA-cultures allowed the identification of four differentially expressed polypeptides, including two putative pathogenesis-related proteins and one putative glutathione S-transferase. For JA-cultures, three additional differentially expressed polypeptides were identified. 相似文献